Molecular Markers for HemePath

Question 1

Castleman Lymphadenopathy

Answer 1

CD123+ (interfollicular plasmacytoid monotcytes)

Question 2

Infectious Mononucleosis

Answer 2

CD30+

LMP+ or EBER+

Question 3

Dermatopathigc lymphadenopathy

Answer 3

(interdigitating dendritic cells)
S100+
Fascin+
CDa1-

Langerhands cels:
S100+
CD1a+
Langerin/CD207

Histiocytes:
CD4+
Lysozyme +
Contain melanin (Fontana-Masson +)

Question 4

Histiocytic Necrotizing Lymphadenitis

aka, Kikuchi-Fujimoto Disease

Answer 4

CD68+

Plasmacytoid dendritic cells:
CD123+
CD303+
Tcl-1 +

Question 5

Rosai Dorfman Disease

Sinus Histiocytosis with Massive Lymphadenopathy

Answer 5

S100+
CD68+
Lysozyme +
PLAP+
CD1a -

Question 6

CLL/SLL

Answer 6

CD20+
CD5+
CD23+
BCL2+
CD200+

BCL1 -
CD10 -

Question 7

Prognostic Factors of CLL/SLL

Answer 7

Favorable:
CD38(-)
Zap70(-)
HYPERmutated IgVH

Adverse
CD38(+)
Zap70(+)
NONmutated IgVH

Question 8

Cytogenetics SLL

Answer 8

del(13q)

Remember that 13 is usually unlucky…except here

Question 9

Follicular Lymphoma

Answer 9

CD10+
BCL6+
HGAL+
LMO+

t(14;18)

Question 10

Mantle Cell Lymphoma

Answer 10

CD20+
CD5+
CD23 -
BCL1+
Sox11+
FMC-7+

High mitotic rate = worse prognosis

Question 11

Mantle Cell Lymphoma

Molecular

Answer 11

t(11;14)

Results in CyclinD1 overexpression

Question 12

Marginal Zone Lymphoma

Molecular

Answer 12

t(11;18)

***Resistant to Abx therapy

Question 13

Waldenstrom Macroglobulinemia/Lymphoplasmacytic Lymphoma

Answer 13

MYD88 L265P

Question 14

Burkitt Lymphoma (Molecular)

Answer 14

**Translocations with Chr. 8 (MYC)
t(8;14) –> MYC/IGH
t(2;8) –> MYC/kappa
t(8;22) –> MYC/lambda

Question 15

Burkitt Lymphoma

Answer 15

CD20+
CD10+
Bcl6+
MYC+
Ki-67 (100% of cells)

Bcl2-

Question 16

Burkitt-Like Lymphoma with 11q abberation

Answer 16

11q alteration

Question 17

AML with nl to high Plt, Marrow dysplasia

Answer 17

inv(3)

Question 18

AML with neurophilic differentiation

Answer 18

t(8;21)

RUNX1-RUNX1T1 Driven Transformation

Question 19

APL

Answer 19

t(15;17)

PML-RARA

Question 20

AML with megakaryoblastic features

Answer 20

Trisomy 21

Question 21

t-AML

Answer 21

Assoc. with alkylating agents or topoisomersae inhibitors

Question 22

Germinal Center Origin

Answer 22

CD10+

Question 23

Hairy Cell Leukemia

Answer 23

CD20+
CD25+
Annexin A1+

Question 24

CML

Answer 24

t(9;22)

Necessary for diagnosis!

Question 25

CD5+/CD10+?

CD5-/CD10-?

Answer 25

CD5+/CD10+ = CLL (CD23+)
CD5+/CD10+ = Mantle Cell (Cyclin D1+, CD23-)
Follicular = CD5-/CD10+

CD5-/CD10-?
Marginal Zone and Lymphoplasmacytic Lymphoma