Molecular Genetics: Transformation Flashcards
1
Q
Heat shocking the cells at 42 C purpose?
A
Allows the cells to expand and uptake the exogenous DNA
2
Q
LB growth media purpose
A
Facilitates recovery of the bacteria and allows expression of the selectable marker.
3
Q
Arabinose Absent
A
Ara C protein blocks the promoter and prevents transcription
4
Q
Arabinose present
A
Arabinose binds to the Ara C protein which causes a conformational change and RNA polymerase is recruited to the promoter resulting in transcription.
