Molecular Genetics Flashcards
-Pentose, phosphate, base makes up
-RNA vs DNA sugar base
-DNA
-DNA has H, RNA has OH
Where does DNA replicate?
Where is RNA transcribed?
Where is RNA translated?
-nucleus
-nucleus; cytoplasm
-Protein K or magnetic beads used to
-Chloroform and Na acetate is what method
-extract DNA
-chemical
-Chaotropic salt
-ethanol
purifies DNA, protects NA
purifies DNA, precipates large biomolecules
-DNA is what charge?
-Ethidirum bromideis used as
-DNA is negatively charged, moves to anode
-dye in buffer
-larger the fragment, —-
-bands cut out from gel called
-to separate RNA, what do you use?
-slower it moves
-elution
-PAGE
dsDNA to ssDNA is called
nucleic acid hybridization
southern blot
-genome to ID sequence, electrophoresis, blot, blotted paper placed in bag to label it, rinse
PCR
-Taq DNA pol
-components
-synthesizes DNA or gene of interest using existing strand by adding dNTPs
-Taq, primers, dNTPs,buffer, DNA
-what specimen required for LAMP
-rtPRC
-saliva
-measures PCR product in each cycle
Traditional PCR vs rtPCR
-traditional = detection, DNA bands, primer increases
-rtPCR=probes or dyes (not primers), peak on graph (not bands), quantify it
-SYBR and TaqMan are
-rtPCR assays
SYBR = no sequence specificity
TaqMAn= high specificity
Which rtPCR is more specific?
TaqMAN
-removes RNA primers
-extends primers
-DNA pol I
-DNA pol II
autosomal codominant
blood group genes
