Molecular diagnostics

Question 1

DNA is double stranded and consists of 4 components. what are they?

Answer 1

Guanine, Adenine, cytosine, tyrosine

Question 2

RNA is single stranded and consists of 4 components. What are they?

Answer 2

Guanine, Adenine, cytosine, Uracil

Question 3

Which test is used to detect proteins?

Answer 3

Western blot

Question 4

Which test is used to detect RNA sequences?

Answer 4

Northern blot

Question 5

Which test is used to detect DNA sequences?

Answer 5

Southern blot

Question 6

What chemical is used to to denature DNA?

Answer 6

sodium hydroxide

Question 7

during viral assembly, viral envelopes are developed how?

Answer 7

from a cell membrane during budding

Question 8

What are the 3 major steps of PCR?

Answer 8

Denaturation: 94-96
Annealing: 50-70
Extension: 68-75

Question 9

What reagents are used in RT-PCR

Answer 9

SYBR green

Taqman

Question 10

This technique amplifies a target sequence?

Answer 10

PCR

Question 11

This technique uses flourescent markers and amplification?

Answer 11

RT-PCR

Question 12

This refers to the conditions that are ideal for binding of a probe to DNA or RNA

Answer 12

Stringency

Question 13

The effect of high stringency

Answer 13

the probe will not bind to the target

Question 14

The effect of low stringency

Answer 14

the probe will bind to unrelated targets

Question 15

Enzyme that extends primers

Answer 15

polymerase

Question 16

directs DNA synthesis to a specific region

Answer 16

primer

Question 17

buffer that maintains pH

Answer 17

tris buffer

Question 18

deoxynuceptides, polymerase and magnesium are all required for what testing?

Answer 18

PCR

Question 19

Which component is important in a PCR reaction to ensure the amplification is specific?

Answer 19

primers