Molecular biology and Medicine

Question 1

What are the steps in cloning?

Answer 1

Digest genome –> separate fragments –> clone –> sequence –> assemble

Question 2

What are the 3 sites in bacteria that we are concerned with?

Answer 2

• Polylinker sites
• Antibiotic/ampicillin-resistant gene
• Origin of replication

Question 3

What method is used for cloning when we use bacteria?

Answer 3

• Lac operon.
• IPTG is used
• LacZ gene is replaced with the gene of interest

Question 4

What is trimethylaminuria?

Answer 4

Bacteria in the gut convert choline to trimethylamine (which smells like fish) – in normal people, this is converted into trimethylamine N-oxide by flavin-containing monooxygenase 3 (FMO3).

However, in people with trimethylaminuria, there is a mutation in FMO3, so trimethylamine accumulates – so it smells like fish.