molecular bio 1 Flashcards
The biotechnology used as a copy machine for DNA is known as
polymerase chain reaction
Which is a a component needed for PCR?
Nucletoides, DNA polymerase. primer
True or False: Taq Polymerase is often used in PCR because it is resistant to acidity.
false
In the denaturation step, ______ is utilized to separate DNA strands.
heat
In the annealing step, ________ binds to the specific segment of DNA to be amplified.
primer
In the synthesis step, _________ uses nucleotides as its building material to amplify the DNA.
DNA polymerase
True or False: Real-time reverse transcription PCR is used routinely used for viral testing.
true
Taq polymerase is used in PCR experiment because the optimal ________ of the enzyme is much more elevated than other DNA polymerases.
temperature
Which of these is a step in PCR?
DNA Synthesis
Annealing
Denaturation
The denaturation step of PCR requires the temperature to ______ while the annealing step requires the temperature to ______.
increase, decrease
The type of enzyme needed in PCR is known as what?
DNA polymerase
Assuming one starts with a single doubled-stranded DNA molecule, how many double-stranded DNA molecules would be there be after three rounds of PCR?
8
True or False: PCR can only occur in cells.
false
An enzyme that converts RNA into DNA is called _________.
reverse transcripton
