Mol Bio and Genetics mock exam q's

Question 1

Okazaki fragments are joined together by:
a.
DNA Ligase
b.
DNA polymerase
c.
RNA ligase
d.
Primase
e.
DNA ligase and DNA polymerase

Answer 1

A) DNA ligase

Question 1

In the usual flow of genetic information, which of the following best represents the expression of genes?
a.
Protein → RNA → DNA
b.
DNA → RNA → Protein
c.
RNA→ DNA → Protein
d.
DNA → Protein → RNA

Answer 1

B) DNA -> RNA -> Protein

Question 2

Leader sequences on mRNA molecules contain signals that:
a.
Prevent enzymes from degrading the new synthesized mRNA
b.
Inhibit ribosome binding until the appropriate time
c.
Initiate chain termination
d.
Allow the ribosomes to be properly positioned to translate the message
e.
Allow RNA polymerase to locate the position of the start codon

Answer 2

D) Allow the ribosomes to be properly positioned to translate the message

Question 3

Which of the following is true about Introns found within human genes? select one or more
a.
They are sequences that code for protein domains and are alternatively spliced to form new
proteins
b.
Sequences that do not code for protein and have no known function in the genome
c.
Sequences that do not code for proteins but can be alternatively spliced during mRNA processes to generate different protein products
d.
Remnants of older life forms and are present in all living organisms
e.
Usually longer in length compared to exons
f.
They are sometimes present in messenger RNA due to a mutation at the splicing site.

Answer 3

C, E, and F

Question 4

Which of the terms below refer to parts of our genome which are translated into proteins:
select one or more
a.
Open reading frames
b.
Introns
c.
Codons
d.
Pseudogenes
e.
the exosome
f.
the transcriptosome
g.
coding exons
h.
untranslated regions

Answer 4

A, E and G

Question 5

The anitibiotic Puromycin inhibits which of the following processes?
a.
Transcription
b.
Translation
c.
Replication
d.
Recombination

Answer 5

B) Translation

Question 6

When the DNA sequence below is transcribed, which mRNA sequence is most likely to be generated form the template strand?
5’ GCG ATA AGC TGC GAA3’
3’ CGC TAT TCG ACG CTT5’

a.
5’CGC UAU UAC UGC CAA3’
b.
5’GCG AUA AGC UGC GAA3’
c.
3’CGC UAU UAC UGC CAA5’
d.
3’GCG AUA AGC UGC GAA5’

Answer 6

B) 5’GCG AUA AGC UGC GAA3’

Question 7

Which of the following statements concerning nucleotide excision repair is false?
a.
It is a type of mismatch repair
b.
It involves a nuclease
c.
It involves a DNA polymerase
d.
It is implicated in xeroderma pigmentosum

Answer 7

A) It is a type of mismatch repair

Question 8

Which of the following statements regarding the function and characteristics of P53 protein are true?
a.
it increases DNA replication
b.
it speeds up cell division
c.
it causes tumour formation
d.
it can activate apoptosis
e.
it activates P21 protein
f.
it activates P35 protein
g.
it carries out specific gene transcription
h.
it forms a tetramer complex

Answer 8

D, E, G and H

Question 9

Eukaryotic mRNA is synthesised by;
a.
RNA polymerase 1
b.
DNA polymerase 1
c.
RNA polymerase 2
d.
DNA polymerase 2
e.
RNA polymerase 3

Answer 9

C) RNA polymerase 2

Question 10

Open samples used for cytogenetic analysis invariably involve samples of:
a.
Amniotic fluid.
b.
Product of conception.
c.
Peripheral blood.
d.
Bone marrow.
e.
Cerebrospinal fluid

Answer 10

b) product of conception

Question 11

Riboprobes are pieces of nucleic acid which are used to detect
a.
RNA molecules
b.
DNA molecules
c.
Protein molecules
d.
Both a and b
e.
none of the above

Answer 11

d) Both a and b

Question 12

What protein(s) is/are involved in the spindle assembly checkpoint?
a.
Cohesin.
b.
Securin.
c.
Separase.
d.
All of the above.
e.
Only (a) and (b).

Answer 12

d) All of the above.

Question 13

Which of the following is NOT due to anomalies in autosomal chromosomes?
a.
Turner syndrome.
b.
Down’s Syndrome.
c.
Edwards Syndrome.
d.
Patau Syndrome.
e.
Cri-du-chat Syndrome

Answer 13

a) Turner syndrome