Module 6

Question 1

Describe how you could produce a calibration curve for glucose

Answer 1

• use distilled water and glucose solutions to produce dilutions
• add benedicts solution and heat in a water bath for 5 minutes
• use a know volume of a solution
• record absorbance using a colorimeter
• plot concentration of solution against absorbance

Question 2

Describe how you would determine the concentration of glucose in a urine sample using your calibration curve

Answer 2

• sample with same volume as those used to produce calibration curve
• add same volume of Benedict’s solution and heat in a water bath at the same temperature for the same time
• read off concentration (x axis) against absorbance (y axis)

Question 3

a level knowledge!!

Give 2 reasons why a weight loss programme could be used to treat type 2 diabetes but not type 1 (2)

Answer 3

• type 1 don’t produce insulin
• in type 2, receptors lack sensitivity to insulin
• weight not linked to type 1 diabetes