module 4 Flashcards
Define Biodiversity
- The variety of different species
- The variation within a species
- The variation of cell types
- Within a single multicellular organism
Define a gene
Section of DNA, located at the locus, whose base sequence determines the sequence of amino acids in a polypeptide and functional RNA [rRNA and tRNA]
Give three differences between prokaryotic DNA and eukaryotic DNA
P – short, circular, not associated with histone proteins
E – long, linear, associated with histones
Describe the DNA in chloroplasts and mitochondria
Short, circular, not associated with histones [same as prokaryotes]
Give three words to describe the genetic code
Non-overlapping, universal, degenerate
Why is the genetic code evidence of evolution?
All species have the same genetic code - universal
How could the DNA and so RNA base sequence change and there be no change to protein tertiary structure?
The mutation may be a degenerate code. E.g. UAU and UAC are both Tyrosine. The Hydrogen, ionic and disulfide bonds would be the same.
Define an exon
Sequences within a gene that code for amino acid sequence. Always separated by at least one intron in eukaryotes
Define an intron
Non-coding multiple repeats, found between genes in eukaryotes
Define a genome
Complete set of genes in a cell
Define a proteome
Full range of proteins that a cell is able to produce
How is mRNA different to DNA?
Uracil replaces Thymine. Single stranded. No introns in eukaryotes
Describe Transcription
- DNA helicase breaks Hydrogen bonds
- DNA bases exposed on template strand.
- RNA polymerase allows complementary RNA bases to align [A-U, G-C] and joins phosphodiester bonds.
- Pre mRNA spliced to form mRNA.
Describe tRNA
Single strand RNA, held in a clover shape by Hydrogen bonds. Carries a specific Amino acid, dependent on anticodon sequence.
Describe Translation
-Ribosome binds to mRNA at start codon
- tRNA carries a specific aa dependent on anticodon sequence.
- If anticodon complementary to codon then amino acid will form peptide bond with other aa to form a polypeptide.
- Formation of peptide bond requires ATP.
- Two tRNA within each Ribosome at one time. - Continues until stop codon of mRNA reached
Mutations can spontaneously arise
during DNA replication. What can
increase the likelihood of mutations?
Mutagenic agents e.g. UV light, X Rays, Radiation or chemical carcinogens. Some viruses or bacteria can increase mutations.