Module 2 Part 2 Flashcards

1
Q

Easy to use, low cost
Dry and brittle

A

Cellulose Acetate

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2
Q

M spike

A

Multiple myeloma

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3
Q

Capable of responding to one specific ion

A

ISE

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4
Q

Two Electrodes

A

Detecting and standard

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5
Q

Types of Electrode

A

Ph electrode
Ion selective
Reference
Indicator
pCO2

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6
Q

Talks about lags / variation

A

Hysteresis

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7
Q

used at temp greater than 60c

A

Silver chloride electrode

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8
Q

saturated with mercury chloride

A

Calomel electrode

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9
Q

how potentials exist

A

interface bet. metals and ions of that metal

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9
Q

electric potential is measured by

A

ph meter

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10
Q

how long does ISE release results

A

5-30 mins

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11
Q

current flow produced by oxidation0reduction reaction

A

amperometry

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12
Q

has no opening

A

glass electrode

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13
Q

plastic jacket filled with sodium bicarbonate

A

pCO2 electrode

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14
Q

method is used to measure chloride in serum, CSF or sweat

A

coulometry

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15
Q

used for chloride determination

A

coulometry and amperometry

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16
Q

immobilized enzymes that can catalyze specific chemical reaction

A

enzyme elecrtrode

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17
Q

movement or transport ions

A

electromigration method

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18
Q

potential applied to an electrochemical cell and resting current measured

A

voltammetry

19
Q

how does electrophoresis separate?

A

basis of electrical charge of particle and medium

20
Q

high-voltage

A

50-200 volts

21
Q

has either (+) or (-) charge

A

amphoteric

22
Q

ISOENZYMES of analytes

A

CK, LD , ALP

23
Q

power supply moves from

A

current-medium-heat

24
Q

used by yellow pipette tip , 2-10 microliters

A

agarose gel

25
Q

migration of small ions

A

iontophoresis

26
Q

separates protein based on surface charge and mol size

A

strach gel

27
Q

relative percent of protein bands: A1 globulin

A

2-5

28
Q

relative percent of protein bands: A2 globulin

A

7-13

29
Q

uses homogenous solution without support medium

A

frontal electrophoresis

30
Q

biuret reaction quantification

A

colorimetric

31
Q

most reliable way of quantification

A

densitometry

32
Q

narrow-bone fused silica capillaries

A

capillary electrophoresis

33
Q

bulk of liquid toward cathode upon application of electric field

A

electro-osmotic floe

34
Q

slows down movement of proteins

A

electroendosmosis

35
Q

produced erroneously high results

A

hemolysis

36
Q

consider colligative properties

A

electrophoresis

37
Q

low ionic strengh: ____: ____

A

more current : fast mobility

38
Q

separation of soluble components; physiochemical properties

A

chromatography

39
Q

planar chromatography - types

A

paper and TLC

40
Q

column chromatography 0types

A

gas and liquid

41
Q

no migration

A

stationary phase

42
Q

migration phase

A

mobile

43
Q

differential migration

A

2 phase: stationary and mobile

44
Q

separating and identifying mixtures that are colored

A

paper chormatogrpahy