Module 2 Part 2 Flashcards

1
Q

Easy to use, low cost
Dry and brittle

A

Cellulose Acetate

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2
Q

M spike

A

Multiple myeloma

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3
Q

Capable of responding to one specific ion

A

ISE

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4
Q

Two Electrodes

A

Detecting and standard

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5
Q

Types of Electrode

A

Ph electrode
Ion selective
Reference
Indicator
pCO2

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6
Q

Talks about lags / variation

A

Hysteresis

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7
Q

used at temp greater than 60c

A

Silver chloride electrode

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8
Q

saturated with mercury chloride

A

Calomel electrode

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9
Q

how potentials exist

A

interface bet. metals and ions of that metal

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9
Q

electric potential is measured by

A

ph meter

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10
Q

how long does ISE release results

A

5-30 mins

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11
Q

current flow produced by oxidation0reduction reaction

A

amperometry

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12
Q

has no opening

A

glass electrode

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13
Q

plastic jacket filled with sodium bicarbonate

A

pCO2 electrode

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14
Q

method is used to measure chloride in serum, CSF or sweat

A

coulometry

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15
Q

used for chloride determination

A

coulometry and amperometry

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16
Q

immobilized enzymes that can catalyze specific chemical reaction

A

enzyme elecrtrode

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17
Q

movement or transport ions

A

electromigration method

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18
Q

potential applied to an electrochemical cell and resting current measured

A

voltammetry

19
Q

how does electrophoresis separate?

A

basis of electrical charge of particle and medium

20
Q

high-voltage

A

50-200 volts

21
Q

has either (+) or (-) charge

A

amphoteric

22
Q

ISOENZYMES of analytes

A

CK, LD , ALP

23
Q

power supply moves from

A

current-medium-heat

24
used by yellow pipette tip , 2-10 microliters
agarose gel
25
migration of small ions
iontophoresis
26
separates protein based on surface charge and mol size
strach gel
27
relative percent of protein bands: A1 globulin
2-5
28
relative percent of protein bands: A2 globulin
7-13
29
uses homogenous solution without support medium
frontal electrophoresis
30
biuret reaction quantification
colorimetric
31
most reliable way of quantification
densitometry
32
narrow-bone fused silica capillaries
capillary electrophoresis
33
bulk of liquid toward cathode upon application of electric field
electro-osmotic floe
34
slows down movement of proteins
electroendosmosis
35
produced erroneously high results
hemolysis
36
consider colligative properties
electrophoresis
37
low ionic strengh: ____: ____
more current : fast mobility
38
separation of soluble components; physiochemical properties
chromatography
39
planar chromatography - types
paper and TLC
40
column chromatography 0types
gas and liquid
41
no migration
stationary phase
42
migration phase
mobile
43
differential migration
2 phase: stationary and mobile
44
separating and identifying mixtures that are colored
paper chormatogrpahy