Module 2 Flashcards

1
Q

Term: Cell Membrane Composition

A

Definition: Cell membranes are composed of lipids, proteins, and carbohydrates, forming a selective barrier that controls the movement of molecules between the inside and outside of the cell.

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2
Q

Term: Amphipathic Phospholipids

A

Definition: Phospholipids have both hydrophilic (water-attracting) heads and hydrophobic (water-repelling) tails. They form a bilayer that makes up the cell membrane, providing a barrier to the external environment.

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3
Q

Term: Lipid Bilayer

A

Definition: A structure consisting of two layers of phospholipids where hydrophobic tails face inward, and hydrophilic heads face outward. It acts as a foundational structure of the cell membrane, allowing selective permeability.

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4
Q

Term: Role of Cholesterol in Membranes

A

Definition: Cholesterol is a component of animal cell membranes. It acts as a buffer to maintain membrane fluidity by preventing the membrane from becoming too rigid or too fluid in varying temperatures.

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5
Q

Term: Integral Membrane Proteins

A

Definition: Proteins that are permanently embedded in the cell membrane. They are crucial for functions such as transport, acting as receptors, and maintaining cell structure.

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6
Q

Term: Peripheral Membrane Proteins

A

Definition: These proteins are temporarily attached to the lipid bilayer or integral proteins through weak non-covalent interactions. They can be involved in signaling or maintaining the cell’s

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7
Q

Q: What is the role of the nuclear envelope in eukaryotic cells, and how does it contribute to cellular function?

A

A: The nuclear envelope surrounds the nucleus, separating it from the cytoplasm. It contains nuclear pores that regulate the exchange of materials (like RNA and proteins) between the nucleus and the rest of the cell, facilitating gene expression and DNA protection.

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8
Q

Q: How do mitochondria and chloroplasts support the endosymbiotic theory of evolution?

A

A: Both mitochondria and chloroplasts contain their own DNA and reproduce independently of the cell, similar to bacteria. This supports the endosymbiotic theory, which suggests these organelles evolved from free-living prokaryotes that were engulfed by ancestral eukaryotic cells.

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9
Q

Q: What is a vesicle, and how does it function in transport within the endomembrane system?

A

A: A vesicle is a small, membrane-bound sac that transports materials within the cell. Vesicles carry proteins and lipids between organelles in the endomembrane system (such as the ER, Golgi apparatus, and plasma membrane) through processes like exocytosis and endocytosis.

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10
Q

Q: What is the primary difference between autotrophic and heterotrophic organisms at the cellular level?

A

A: Autotrophs (like plants) produce their own food through photosynthesis in chloroplasts, using sunlight to convert CO2 into glucose. Heterotrophs (like animals) cannot produce their own food and must consume other organisms for energy.

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11
Q

Q: What are the key differences between plant and animal cells at the cellular level?

A

A: Plant cells have a cell wall, chloroplasts, and a large central vacuole, while animal cells do not. Plant cells also use chloroplasts for photosynthesis, while animal cells rely solely on mitochondria for energy production.

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12
Q

Q: How does the sodium/potassium pump maintain the electrochemical gradient in nerve cells?

A

A: The sodium/potassium pump actively transports 3 Na+ ions out of the cell and 2 K+ ions into the cell, creating an electrochemical gradient. This gradient is essential for transmitting nerve impulses and maintaining membrane potential.

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13
Q

Q: What are the three main functions of the Golgi apparatus in a cell?

A

A: The Golgi apparatus functions to: 1) Modify proteins and lipids from the ER, 2) Sort these molecules for transport to their final destinations, and 3) Synthesize some cellular carbohydrates, such as polysaccharides used in the cell wall.

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14
Q

Q: How do lysosomes contribute to the recycling of cellular materials, and what happens if they malfunction?

A

A: Lysosomes contain enzymes that break down cellular debris, damaged organelles, and foreign particles. If lysosomes malfunction, waste materials can accumulate, leading to diseases like lysosomal storage disorders (e.g., Gaucher disease).

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15
Q

Q: Explain the structure of the phospholipid bilayer and how it influences membrane fluidity.

A

A: The phospholipid bilayer consists of two layers of phospholipids with hydrophobic tails facing inward and hydrophilic heads facing outward. This structure allows the membrane to be flexible while providing a barrier to most polar molecules. The presence of cholesterol within the bilayer further modulates membrane fluidity.

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16
Q

Q: What is the difference between primary and secondary active transport?

A

A: Primary active transport directly uses energy from ATP to move molecules against their concentration gradient, such as the sodium/potassium pump. Secondary active transport uses the energy stored in the gradient of one molecule (e.g., Na+) to drive the transport of another molecule (e.g., glucose) without directly using ATP.

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17
Q

Q: What are the three main components of cell membranes, and what is their function?

A

A: Cell membranes are composed of lipids, proteins, and carbohydrates. Lipids provide a hydrophobic barrier, proteins act as transporters and receptors, and carbohydrates are involved in cell recognition and communication.

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18
Q

Q: What is the primary function of the plasma membrane, and how does it maintain cellular homeostasis?

A

A: The plasma membrane acts as a selective barrier that controls the movement of molecules into and out of the cell, maintaining homeostasis by regulating nutrient intake, waste removal, and communication with other cells.

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19
Q

Q: Name two organelles involved in harnessing energy in cells and describe their specific roles.

A

A: Mitochondria generate ATP through cellular respiration, while chloroplasts in plant cells convert solar energy into chemical energy (glucose) via photosynthesis.

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20
Q

Q: What is the basic unit of life according to cell theory, and what are the three principles of cell theory?

A

A: The basic unit of life is the cell. The three principles are: 1) All living organisms are composed of cells. 2) The cell is the basic unit of life. 3) All cells come from preexisting cells.

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21
Q

Q: What are the three primary components of the endomembrane system, and what are their functions?

A

A: The nuclear envelope protects the DNA, the endoplasmic reticulum (ER) synthesizes proteins and lipids, and the Golgi apparatus modifies, sorts, and packages proteins for transport.

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22
Q

Q: Define diffusion and explain how it drives passive transport in cells.

A

A: Diffusion is the movement of molecules from an area of high concentration to low concentration, driving passive transport, where molecules move across membranes without energy input, down their concentration gradient.

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23
Q

Q: What is osmosis, and how does it differ from regular diffusion?

A

A: Osmosis is the diffusion of water across a selectively permeable membrane. It differs from regular diffusion because it specifically involves the movement of water to balance solute concentrations on both sides of the membrane.

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24
Q

Q: How does active transport differ from passive transport, and what energy source does active transport require?

A

A: Active transport requires energy (usually from ATP) to move molecules against their concentration gradient, whereas passive transport does not require energy and moves molecules along their concentration gradient.

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25
Q

Q: Describe the function of the sodium/potassium pump and explain its importance in maintaining cellular function.

A

A: The sodium/potassium pump moves Na+ out of the cell and K+ into the cell against their concentration gradients using ATP. This pump is essential for maintaining the electrochemical gradient necessary for nerve impulses and muscle contractions.

26
Q

Q: What are the functions of the Golgi apparatus, and how does it contribute to protein processing?

A

A: The Golgi apparatus modifies proteins and lipids from the ER, sorts them for transport to their final destinations (inside or outside the cell), and synthesizes important cellular carbohydrates. It acts like a post office, packaging and shipping proteins.

27
Q

Q: What role do lysosomes play in maintaining cellular health, and what can go wrong if they malfunction?

A

A: Lysosomes contain digestive enzymes that break down cellular waste, damaged organelles, and pathogens. If lysosomes malfunction, it can lead to the accumulation of waste products, causing diseases like Tay-Sachs.

28
Q

Q: What is turgor pressure, and how does it affect plant cells?

A

A: Turgor pressure is the pressure exerted by water inside the vacuole against the plant cell wall. It helps maintain the structure of plant cells, keeping them rigid. A loss of turgor pressure causes plants to wilt.

29
Q

Q: Explain the role of mitochondria in cellular respiration and how they contribute to energy production.

A

A: Mitochondria are the powerhouses of the cell, where glucose is broken down into ATP through cellular respiration. This process involves glycolysis, the citric acid cycle, and oxidative phosphorylation, converting chemical energy into a form the cell can use.

30
Q

Q: How does the endoplasmic reticulum (ER) connect to the nuclear envelope, and what are its two main types?

A

A: The endoplasmic reticulum (ER) is continuous with the nuclear envelope, and it comes in two types: rough ER, which is studded with ribosomes and synthesizes proteins, and smooth ER, which synthesizes lipids and detoxifies toxins.

31
Q

Q: What is the primary difference between rough ER and smooth ER in terms of function?

A

A: Rough ER is involved in protein synthesis due to its ribosomes, while smooth ER is responsible for lipid synthesis, detoxification of harmful substances, and calcium ion storage.

32
Q

Q: What role do ribosomes play in protein synthesis, and where can they be found in the cell?

A

A: Ribosomes assemble amino acids into proteins during translation. They can be found either free in the cytoplasm or attached to the rough ER, where they produce proteins for secretion or use within membranes.

33
Q

Q: What is exocytosis, and how does it help cells maintain homeostasis?

A

A: Exocytosis is the process by which vesicles fuse with the plasma membrane to release substances outside the cell, such as waste products or signaling molecules. This helps maintain homeostasis by balancing the intake and release of materials.

34
Q

Q: Define endocytosis and differentiate between phagocytosis and pinocytosis.

A

A: Endocytosis is the process of taking materials into the cell by engulfing them in a vesicle. Phagocytosis (“cell eating”) involves engulfing large particles, while pinocytosis (“cell drinking”) involves engulfing liquids and small solutes.

35
Q

Q: What role does cholesterol play in regulating membrane fluidity in animal cells?

A

A: Cholesterol acts as a buffer in the cell membrane, stabilizing the membrane at high temperatures and preventing it from becoming too rigid at low temperatures. It maintains optimal membrane fluidity.

36
Q

Q: What is the difference between integral membrane proteins and peripheral membrane proteins in terms of association with the membrane?

A

A: Integral membrane proteins are permanently embedded in the lipid bilayer, often spanning the membrane, while peripheral membrane proteins are temporarily associated with the membrane, typically through interactions with integral proteins.

37
Q

Q: Describe the amphipathic nature of phospholipids and how it influences membrane structure.

A

A: Phospholipids have a hydrophilic (water-attracting) head and two hydrophobic (water-repelling) tails. This amphipathic nature drives the formation of the lipid bilayer, with hydrophobic tails facing inward and hydrophilic heads facing outward.

38
Q

Q: How do transporters function in the cell membrane, and what types of molecules do they typically transport?

A

A: Transporters are proteins that move ions or molecules across the membrane, often using energy. They transport a variety of molecules, including ions like sodium (Na+), potassium (K+), and small organic molecules like glucose.

39
Q

Q: What is the difference between simple diffusion and facilitated diffusion, and what types of molecules use each process?

A

A: Simple diffusion involves small, non-polar molecules (like O2 and CO2) passing directly through the membrane, while facilitated diffusion requires transport proteins to move larger or polar molecules (like glucose) across the membrane.

40
Q

Q: Explain how secondary active transport works and provide an example.

A

A: Secondary active transport uses the energy from the movement of one molecule down its gradient to drive the movement of another molecule against its gradient. An example is the sodium-glucose cotransporter in the intestines.

41
Q

Q: What is Gibbs free energy (ΔG), and how does it determine the spontaneity of a reaction?
Q: What is Gibbs free energy (ΔG), and how does it determine the spontaneity of a reaction?

A

A: Gibbs free energy is the amount of energy available to do work in a system. If ΔG < 0, the reaction is spontaneous (exergonic), releasing energy. If ΔG > 0, the reaction is non-spontaneous (endergonic), requiring an input of energy.

42
Q
  1. Q: What is the difference between exergonic and endergonic reactions?
A

A: Exergonic reactions release energy and have a negative ΔG, making them spontaneous. Endergonic reactions require energy input and have a positive ΔG, meaning they are non-spontaneous.

43
Q
  1. Q: How do enzymes speed up biochemical reactions?
A

A: Enzymes lower the activation energy (EA) needed for a reaction to proceed by stabilizing the transition state, allowing the reaction to occur more quickly without changing the reaction’s ΔG.

44
Q
  1. Q: What is the role of the active site in an enzyme?
A

A: The active site is the region on the enzyme where the substrate binds. The shape and chemical environment of the active site facilitate the conversion of the substrate into the product, lowering activation energy.

45
Q
  1. Q: What is energetic coupling, and why is it important in metabolism?
A

A: Energetic coupling uses the energy released from an exergonic reaction (e.g., ATP hydrolysis) to drive an endergonic reaction, making non-spontaneous processes like muscle contraction or biosynthesis possible.

46
Q
  1. Q: What is an example of an enzyme-catalyzed reaction?
A

An example is the breakdown of hydrogen peroxide (H₂O₂) by the enzyme catalase into water (H₂O) and oxygen (O₂). The enzyme speeds up the decomposition reaction.

47
Q

Q: What is a transition state in a chemical reaction?

A

A: The transition state is a high-energy intermediate stage during a chemical reaction when old bonds are breaking, and new bonds are forming. It represents the point of highest energy along the reaction path.

48
Q
  1. Q: How do temperature and pH affect enzyme activity?
A

A: Enzymes have an optimal temperature and pH at which they function best. Deviations from these conditions can cause denaturation (loss of shape), reducing the enzyme’s ability to bind substrates effectively.

49
Q

Q: What is the difference between a reversible and irreversible enzyme inhibitor?

A

A: Reversible inhibitors bind to enzymes through weak, non-covalent interactions and can dissociate. Irreversible inhibitors form strong covalent bonds with enzymes, permanently disabling their activity.

50
Q

How does atp function as an energyy carrier in cells?

A

A: ATP stores energy in its high-energy phosphate bonds. When ATP is hydrolyzed to ADP (adenosine diphosphate) and an inorganic phosphate (Pi), it releases energy that can be used for cellular processes.

51
Q
  1. Q: What is the role of RNA polymerase II, and why is it a target of toxins like amatoxins?
A

A: RNA polymerase II synthesizes mRNA, essential for protein production. Amatoxins from Amanita phalloides inhibit RNA polymerase II, blocking mRNA synthesis and causing cell death.

51
Q
  1. Q: How does enzyme specificity work?
A

A: Enzymes are highly specific, meaning they bind to specific substrates due to the precise shape and chemical properties of their active sites, which match the substrate like a “lock and key.”

52
Q

-What are allosteric enzymes, and how are they regulated?

A

A: Allosteric enzymes are regulated by molecules that bind to sites other than the active site (allosteric sites). This binding changes the enzyme’s shape, either activating or inhibiting its activity.

53
Q
  1. Q: What is the effect of competitive inhibition on enzyme activity?
A

A: In competitive inhibition, an inhibitor molecule competes with the substrate for binding at the enzyme’s active site, reducing the rate of reaction until the substrate concentration increases sufficiently to outcompete the inhibitor.

54
Q
  1. Q: What is the Michaelis-Menten model of enzyme kinetics?
A

A: The Michaelis-Menten model describes the relationship between substrate concentration and reaction rate. It introduces the Michaelis constant (Km), which is the substrate concentration at which the reaction rate is half of its maximum (Vmax).

55
Q
  1. Q: What is a cofactor, and why is it important for enzyme function?
A

A: A cofactor is a non-protein molecule (often a metal ion or coenzyme) required for an enzyme’s activity. It assists in stabilizing the enzyme-substrate complex or participates in the chemical reaction.

56
Q
  1. Q: What is a cofactor, and why is it important for enzyme function?
A

A: A cofactor is a non-protein molecule (often a metal ion or coenzyme) required for an enzyme’s activity. It assists in stabilizing the enzyme-substrate complex or participates in the chemical reaction.

57
Q
  1. Q: What is the role of feedback inhibition in metabolic pathways?
A

A: Feedback inhibition occurs when the product of a metabolic pathway inhibits an enzyme involved earlier in the pathway, preventing the overproduction of the product and maintaining balance in the cell

58
Q
  1. Q: What is the role of feedback inhibition in metabolic pathways?
A

A: Feedback inhibition occurs when the product of a metabolic pathway inhibits an enzyme involved earlier in the pathway, preventing the overproduction of the product and maintaining balance in the cell

59
Q
  1. Q: How do anabolic and catabolic pathways differ in terms of Gibbs free energy (ΔG)?
A

A: Anabolic pathways build larger molecules from smaller ones and require energy input (ΔG > 0), while catabolic pathways break down molecules, releasing energy (ΔG < 0).

60
Q
  1. Q: What is activation energy (EA), and how do enzymes influence it?
A

A: Activation energy is the energy barrier that must be overcome for a reaction to proceed. Enzymes lower this energy barrier, allowing the reaction to occur more easily at biological temperatures.