Module 2

Question 1

What is Resolution

Answer 1

The minimum distance between two points in which they can still be viewed as two seperate points.

Question 2

What is magnification and calculation

Answer 2

How many times larger the image is compared to the object.
Magnification= size of image/ size of real object.

Question 3

Give the types of microscopes. (x4)

Answer 3

Optical microscope
Transmission electron microscope
scanning electron microscope
Laser scanning confocal microscope

Question 4

what is a dry mount?

Answer 4

Thin slices or whole specimens are viewed with just the coverslip place on top

Question 5

wet mount

Answer 5

water is added to the specimen before lowering coverslip with mounted needle to prevent aire bubbles forming

Question 6

squash slides

Answer 6

wet mounts where coverslip is pushed down to squash the sample,
Ensure a thin layer is obtained so that light can pass through.

Question 7

Smear slide

Answer 7

sample is smeared across a slide.
coverslip is then placed on top after smearing.

Question 8

eye piece graticule

Answer 8

A small piece of glass with a measurement scale etched on its surface that fits inside a microscope eyepiece.

Question 9

calibrating eyepiece graticule

Answer 9

Line up the eyepice graticule with stage micrometer.
count how many divisions on the eyepice graticule fit into one division on the micrometer scale.
each micrometer division is 10 um, this can be used to calculate what one division on the eyepiece graticule is.

Question 10

what is differential staining?

Answer 10

Many different chemical stains are used to stain a sample. This increases contrast and stain different parts of the cell different colours.

Question 11

Gram Staining

Answer 11

Use of differential staining that allows different bacteria to be visualised.