MODULE 2 Flashcards
Components of Nucleoside
Nitrogenous base + Pentose sugar
Components of Nucleotide
Nitrogenous base + Pentose Sugar + Phosphate (PO4)
Structures:
Adenine(A)
Guanine(G)
Thymine(T)
Cytosine(C)
Uracil(U)
For DNA: A-T, C-G
For RNA: A-U, C-G
Bond between oxygen part of the Phosphate and Pentose sugar
Phosphodiester bond
Hydrogen atom located between a pair of other atoms having a high affinity for electrons; bond is weaker than ionic bond or covalent bond but stronger than van der Waals forces
Hydrogen Bond
Glycosidic bond formed between sugar and nitrogen base
Bond that connects adenine and ribose in the molecule adenosine
N-glycosidic bond
DNA
Pentose
Base
Location
Form
Shape
- 2-deoxyribose
- A=T, C=G
- Nucleus and Mitochondria
- Double stranded
- double, triple, quadruple helix, cruciform
RNA
Pentose
Base
Location
Form
Shape
- Ribose
- A=U, C=G
- Nucleus, Mitochondria, Cytosol
- single stranded
- Cloverleaf, random and hairpin loops, cruciform
Basic unit of heredity and codes for specific products (RNA, proteins)
Gene
Self-replicating unit, holds all genes, condenses DNA
Chromosome
(46 chromosomes = 23 base pairs, 22 somatic, 1 sex pili)
Collection of all genetic information
Genome
Genome of all microorganism in the body
Microbiome
Location of DNA Replication
Nucleus, Mitochondria
Location of DNA Transcription
Nucleus
Location of DNA Translation - mRNA
Cytosol - aids of ribosomes
DNA Replication
DNA-dependent DNA synthesis
DNA Transcription
DNA-dependent RNA synthesis
DNA Translation
Conversion of RNA to proteins
Correct sequence: DNA Replication
Initiation > Elongation> Termination
Enzymes utilized during Initiation stage
unwinding dsDNA
Helicase
Enzymes utilized during Initiation stage
Relieves supercoiling
DNA Gyrase (Topoisomerase II)
Enzymes utilized during Initiation stage
adds RNA primer
Primase
Enzymes utilized during Elongation stage
attach to single strands to prevent re-annealing
SSBP (single-stranded binding protein)
Enzymes utilized during Elongation stage
Adds nucleotides from 5 - 3 direction relative to daughter strand, has exonuclease activity - proofread & correct errors (1 error for 1 billion base pairs)
DNA polymerase
Kinds of DNA Polymerase
1. DNA polymerase α
Primase
Kinds of DNA Polymerase
2. DNA polymerase β
Proof-reading
Kinds of DNA Polymerase
3. DNA polymerase ε
Elongation: LEADING strand
Kinds of DNA Polymerase
4. DNA polymerase δ
Elongation: LAGGING strand
Kinds of DNA Polymerase
5. DNA polymerase γ
Mitchondrial DNA Replication
Enzymes utilized during Termination stage
DNA Ligase - enzyme connects the OKAZAKI fragment
RNA Polymerase
Transcription process
Attaches to consensus sequences
No primers required
Kind of RNA Polymerase
CAAT box
(Hogness box) - Euk
Kind of RNA Polymerase
TATA box
(Pribnow box) - Prok
Post-transcriptional process
Addition of 7-Methylguanosine Cap
1st Step
Protects mRNA from Hydrolysis
Post-transcriptional process
Addition of poly-Adenosine tail
2nd Step
Polyadenylation
Post-transcriptional process
Splicing
3rd Step
Removal of INTRONS (noncoding sequence) from the mRNA
Triplet sequences of nucleotides (seen in RNA only)
CODON - codes specific amino acid
Codon Properties
1 codon code for 1 amino acid
Specific
Codon Properties
multiple codons code for 1 amino acid
Degenerate/ Redundant
Essential for most biological functions, either by performing the function itself (Non-coding RNA) or by forming a template for production of proteins (mRNA).
RNA (Rebonucleic acid)
Types of RNA
rRNA (RNA Polymerase I)
Ribosomal RNA
Types of RNA
mRNA (RNA Polymerase II)
Messenger RNA (Carries Genetic information)
Types of RNA
tRNA (RNA Polymerase III)
TRANSFERS amino acid from **Cytosol **to Ribosomes
3′ end > CCA sequence (where amino acid attached)
Occurs in GOLGI bodies
Post-Translation Modification
-involves Glycosylation and Proteolytic Cleavage
AUG
**START CODON
UAA-UAG-UGA
STOP CODON
-once reached, release factors bind to RIBOSOME > Dissociation
Sum of all chemical process that makes the cells self-perpetuating
METABOLISM
Types of Metabolism
Building up; Energy consuming
ANABOLISM
Types of Metabolism
(cut) Breaking down; Energy producing
CATABOLISM
Types of Metabolism
both have Anabolic and Catabolic parts
AMPHIBOLIC
Energy investment stage
Glycolysis
Glycolysis
enzyme that converts GLUCOSE to Glucose-6-phosphate using 1 ATP > ADP
Hexokinase
Glycolysis
enzyme that converts Fructose-6-phosphate to fructose1-6-biphosphate using 1 ATP > ADP
Phosphofructokinase
enzyme responsible for transfer of Phosphate group
Kinases
enzyme that catalyzes redox reaction
Dehydrogenases
enzymes that convert a molecule from one isomer to another
Isomerases
No. of ATP produced from beta-oxidation of linolenic acid
140
black urine disease caused by homogenistic acid, oxidized into melanin-like compound
Alkaptonuria
defect in phenylalanine hydroxylase
Phenylketonuria
maple syrup disease or branched amino acid aciduria (Val, Leu, Ile)
Alpha-keto acid dehydrogenase
Type of Mutation
coding of a stop codon
Nonsense
Type of Mutation
coding of different amino acid
Missense
Type of Mutation
no change in protein sequence
Silent
Type of Mutation
single base change; may be silent, missense or nonsense
Point
Type of Mutation
Insertion of 1 or more bases in DNA portion
Insertion
Type of Mutation
removal of 1 or more bases
Deletion
Type of Mutation
Insertion or Deletion of a number of bases that is not multiple of 3
Frameshift
Type of Mutation
purine nucleotide is substituted w/purine or pyrimidine w/ pyrimidine
Transitional
Type of Mutation
purine is replaced w/ pyridine or vice versa
Transversional
Nyctalopia (night blindness) and Xerophthalmia
Vitamin A deficiency (Retinol)
Beri Beri/Wernicke-Korsakoff in alcoholics
Vitamin B1 deficiency (Thiamine)
Coenzyme: TPP (thiaminpyrophosphate)
Function: Aldehyde grp transfer
Cheliosis
Vitamin B2 Deficiency (Riboflavin)
Coenzyme: FAD (flavin adenine dinucleotide)
Function: Oxidation or Hydrogen Transfer
Pellagra (diarrhea, dermatitis, delirium)
Vitamin B3 deficiency (Niacin)
Coenzyme: NAD+ (nicotinamide adenine dinucleotide
Function: Oxidation or Hydrogen Transfer
Burning feet syndrome
Vitamin B5 deficiency (Pantothenic acid)
Coenzyme: CoA (coenzyme A)
Function: acetyl group carrier
Peripheral neuropathy
Vitamin B6 deficiency (Pyridoxine)
Dermatitis, alopecia, Lethargy, hallucinations
Vitamin B7/H deficiency (Biotin)
Coenzyme: Biotin
Function: Addition of carboxyl group (carboxylase)
Megaloblastic anemia, spina bifida (fetus)
Vitamin B9 deficiency (Folic acid)
Pernicious Anemia, Megaloblastic anemia
Vitamin B12 deficiency (Cobalamin)
Coenzyme: B12
Function: Methyl group transfer
Scurvy
Vitamin C deficiency (Ascorbic acid)
Rickets (osteomalacia in adults)
Vitamin D deficiency (Calciferol)
nerve and muscle damage, vission problems
Vitamin E deficiency (Tocopherol)
Prolonged bleeding
Vitamin K1 deficiency (phylloquinone) Vitamin K2 deficiency (menaquinone)
Liquid phase remains on the blood after blood coagulation
Serum = plasma (clotting factors)
blood - cellular components (solid)
Plasma
clotting factor 1a
Fibrin
produced by liver mitochondria from acetyl CoA derived fro FA or pyruvate oxidation
Ketone Bodies (sources of energy in peripheral tissues)
acetyl CoA oxidized by TCA cycle
what are the ketone bodies
Acetoacetic acid
β-hydroxybutyric acid
Acetone
Not a ketone body and produce in the Krebs Cycle
Oxaloacetic acid