Module 1 Intro to Histology

Question 1

the study of tissues of the body and how these tissues are arranged to constitute organs

Answer 1

Histology

Question 2

Name the sequence of steps for preparing tissues for study in order

Answer 2

1.) Fixation
2.) Dehydration
3.) Clearing
4.) Infiltration
5.) Embedding
6.) Trimming

Question 3

preserve tissue structure and prevent degradation by enzymes

Answer 3

Fixation

Question 4

two fixatives used for light microscopy

Answer 4

Formalin, Glutaraldehyde

Question 5

what percentage of formaldehyde is used in formalin

Answer 5

37%

Question 6

Tissue is transferred through a series of increasingly concentrated alcohol solutions, ending in 100% which removes all water

Answer 6

Dehydration

Question 7

Alcohol is removed in organic solvents in which both alcohol and paraffin are miscible

Answer 7

Clearing

Question 8

commonly used clearing agent; to displace ethanol from the tissue

Answer 8

Xylene

Question 9

Tissue is placed in melted paraffin until it becomes completely filled with this substance

Answer 9

Infiltration

Question 10

at what temperature should infiltration be done

Answer 10

52 to 60 degrees celsius

Question 11

Paraffin-infiltrated tissue is placed in a small mold with melted paraffin and allowed to harden at room temperature.

Answer 11

Embedding

Question 12

Resulting paraffin block is trimmed to expose the tissue for sectioning on a microtome

Answer 12

Trimming

Question 13

used for sectioning paraffin-embedded tissues for light microscopy

Answer 13

microtome

Question 14

process whereby tissue components are made visible in microscopic sections by direct interaction with a dye or staining solution

Answer 14

Staining