Brainscape's Knowledge GenomeTM

Browse over 1 million classes created by top students, professors, publishers, and experts.


See full index

LABORATORY DIAGNOSIS > MODULE 1 > Flashcards

MODULE 1 Flashcards

0
Q

In collecting specimens, the following should be on the label container EXCEPT:

A. Date and time collected
B. Source of specimen (body location)
C. Name of the medical technologist
D. Identification number of the patient

A

C. Name of the medical technologist

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
1
Q

The following specimen collections are to be kept in room temperature EXCEPT:

A. Blood
B. CSF
C. Specimens for detection of C. trachomatis
D. Specimens for recovery of N. gonorrhea

A

C. Specimens for detection of C. trachomatis

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

TRUE or FALSE: Actual tissue or Fluid Aspirate is always superior than swab.

A

TRUE

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

Non-covalent interaction; R-group within protein.

A. Primary Structure
B. Secondary Structure
C. Tertiary Structure
D. Quaternary Structure

A

C. Tertiary Structure

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

Separation of the protein fractions into discrete bands or zones due to the introduction of filter paper as an anticonvection support medium.

A. Endosmosis
B. Zonal electrophoresis
C. Tiselius
D. Gel filtration

A

B. Zonal electrophoresis

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

Also referred to as electro-osmosis.

A. Endosmosis
B. Zonal electrophoresis
C. Tiselius
D. Gel filtration

A

A. Endosmosis

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

TRUE or FALSE: The buffer flow which carries the proteins with it is through charge.

A

FALSE.

Endosmosis uses mechanical flow instead of charge.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

The following are true of the ionic strength of the buffer EXCEPT:

A. If the electrodes are not properly aligned, the current may be denser on one side of the gel than on the other.

B. It determines the amount of current and movement of the proteins for a fixed voltage.

C. If low, relatively less current is carried by the proteins, which move a shorter distance.

D. If high, less current is carried by the proteins, which move a shorter distance.

A

C. If low, relatively less current is carried by the proteins, which move a shorter distance.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

These support media are used predominantly in clinical laboratories because of ease of use, low cost, and commercial availability.

A. Cellulose acetate and agarose
B. Starch gel and Agarose gel
C. Polyacrylamide gel and cellulose acetate
D. Polyacrylamide gel and starch gel

A

A. Cellulose acetate and agarose

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

The following are true of Gel Filtration EXCEPT:

A. Simplest and mildest of all chromatography techniques
B. Size-exclusion chromatography (SEC)
C. Separate molecules based on differences in size
D. Particles of the largest size penetrate the farthest into the anterior of gel filtration beads.

A

D. Particles of the largest size penetrate the farthest into the anterior of gel filtration beads.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

TRUE or FALSE: In Gel Filtration, the smallest molecules will elute first and the biggest one will last.

A

FALSE.

Big ones will elute first and the smallest ones will elute last.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

The advantage of this method is that biomolecules with even small differences in net surface charge can be separated.

A. Cation Exchange Chromatography
B. Hydrophobic Chromatography
C. Ion-Exchange Chromatography
D. Anion Exchange Chromatography

A

C. Ion-Exchange Chromatography

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

Samples are applied at high salt; eluted with low salt.

A. Cation Exchange Chromatography
B. Hydrophobic Chromatography
C. Ion-Exchange Chromatography
D. Anion Exchange Chromatography

A

B. Hydrophobic Chromatography

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

Based on a specific binding between a protein of interest and another protein that has been covalently linked to the solid support medium of a column.

A. Cation Exchange Chromatography
B. Affinity Chromatography
C. Ion-Exchange Chromatography
D. Anion Exchange Chromatography

A

B. Affinity Chromatography

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

The most common use of affinity chromatography is for ____________________.

A

Purification of recombinant proteins

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

Ultimate reference method for determining concentration proteins.

A. Kjedahl Technique
B. Nitrogen Analysis
C. Copper Sulfate Solution
D. Turbidimetric Methods

A

B. Nitrogen Analysis

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

Process wherein double iodides (potassium and mercuric) forms a colored complex with ammonia in an alkali medium.

A

Nesslerization

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
17
Q

The following statements are true EXCEPT:

A. The Kjedahl Technique is highly accurate but not commonly used in the lab.

B. Protein in a solution absorbs ultraviolet light at 280 nm.

C. In Cation Exchange Chromatography, the positively charged cations are shown while the negative anions are kept.

D. Turbidimetric methods could also be used to detect nucleic acids.

A

C. In Cation Exchange Chromatography, the positively charged cations are shown while the negative anions are kept.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
18
Q

Used to estimate specific gravity and inference protein as well.

A. Kjedahl Technique
B. Copper Sulfate Technique
C. Absorbance
D. Capillary Electrophoresis

A

B. Copper Sulfate Technique

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
19
Q

In turbidimetric method, protein forms a precipitate on the addition of which substance?

A. Ammonia
B. Trichloroacetic acid
C. Copper sulfate
D. Nitrogen

A

B. Trichloroacetic acid

Note: Sulfosalycilic acid can also be used.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
20
Q

Highly specific for proteins and peptide.

A. Colorimetric Method
B. Ion-Exchange Chromatography
C. Turbidimetric Method
D. Affinity Chromatography

A

A. Colorimetric Method

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
21
Q

Used for consistently accurate determination of protein concentration.

A. Colorimetric method
B. Lowry Assay
C. Kjedahl Technique
D. Gl Filtration

A

B. Lowry Assay

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
22
Q

The following are standard dyes for electrophoresis EXCEPT:

A. Ponceau S
B. Amido Black
C. Bromphenol Blue
D. Coomasie Brilliant Blue

A

C. Bromphenol Blue

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
23
Q

Immunologic method that detects proteins in lower concentrations.

A

Radioimmunoassay (RIA) and Enzyme-linked Immunoabsorbent Assay (ELISA)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
24
Q

Detects turbidity produced by precipitation of a reagent antibody with its target protein and is used for measuring major proteins automated immunochemical analyzers.

A

Nephelometry

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
25
Q

Also known as thyroxine-binding prealbumin (TBPA) or transthyretin (TTR).

A. Ceruloplasmin
B. Albumin
C. Prealbumin
D. AAT

A

C. Prealbumin

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
26
Q

Prealbumin plays a significant role in the metabolism of this essential vitamin.

A. Vitamin C
B. Vitamin A
C. Vitamin B12
D. Vitamin D

A

B. Vitamin A

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
27
Q

The complex of retinol, RBP, and transthyretin appears to be assembled in the _____________ of hepatocytes.

A. Mitochondria
B. Golgi Apparatus
C. Endoplasmic Reticulum
D. Nucleus

A

C. Endoplasmic Reticulum

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
28
Q

The following are true of prealbumin EXCEPT:

A. B-pleated sheet conformation
B. Rich in lysine
C. Half-life of roughly 2 days
D. A better early indicator of change in nutritional status than other commonly used markers

A

B. Rich in lysine

Prealbumin is rich in tryptophan.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
29
Q

TRUE or FALSE: True prealbumin is generally below the level of detection by serum electrophoresis, it is best quantified by immunologic measurements as nephelometry.

A

TRUE

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
30
Q

Portion of prealbumin is the source of _____________ component in type I familial amyloidotic polyneuropathy.

A

B-fibrillar amyloid

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
31
Q

Most abundant protein in the plasma constituting up to two thirds of total plasma protein.

A. Ceruloplasmin
B. Albumin
C. Prealbumin
D. Fibrinogen

A

B. Albumin

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
32
Q

Depressions of albumin result in serious imbalance of intravascular oncotic pressure. This loss is manifested clinically by the development of __________________.

A

Peripheral edema

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
33
Q

The following are true of albumin EXCEPT:

A. Congenital absence of albumin generally does not lead to such problems.

B. One of its function is to serve as a mobile repository of amino acids for incorporation into other proteins.

C. The primary sequence of albumin contains five major regions with five peptide loops each.

D. Up to 8% of albumin circulating in normal persons become glycosylated nonenzymatically.

A

C. The primary sequence of albumin contains five major regions with five peptide loops each.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
34
Q

Half life of circulating albumin.

A. ~2 days
B. ~10 days
C. ~17 days
D. ~50 days

A

C. ~17 days

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
35
Q

Most abundant alpha globulin and most important protease inhibitor in plasma.

A

Alpha-1 Antitrypsin (AAT)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
36
Q

AAT is is coded by SERPINA1 on _____________.

A. Chromosome 15
B. Chromosome 20
C. Chromosome 14
D. Chromosome 9

A

C. Chromosome 14

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
37
Q

AAT phenotype susceptible for pulmonary or hepatic disease.

A. SZ phenotype
B. SS homozygous
C. ZZ homozygous
D. MZ phenotype

A

C. ZZ homozygous

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
38
Q

The following are true of the phenotypes for AAT EXCEPT:

A. PiS variant of alele are moderately reduced AAT expression but without any disease associated.

B. Phenotype MZ exhibits with reduced AAT.

C. Majority are Z alele or phenotype ZZ.

D. SS and SZ phenotype suspected of increased lung and hepatic disease.

A

C. Majority are Z alele or phenotype ZZ.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
39
Q

Largest major nonimmunoglobulin protein plasma.

A. Alpha-2 macroglobulin
B. Fibrinogen
C. Ceruloplasmin
D. Prealbumin

A

A. Alpha-2 macroglobulin

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
40
Q

The following are true of alpha-2 macroglobulin EXCEPT:

A. Women have higher levels than men in response to estrogen.

B. Inactivates proteases by complexing with them and forming covalent bonds to them.

C. Combines with hemoglobin released by lysis of red cells in order ro preserve body iron and protein stores.

D. The spectrum of inhibition by AMG is very wide.

A

C. Combines with hemoglobin released by lysis of red cells in order ro preserve body iron and protein stores.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
41
Q

The primary function of this protein is that it combines with hemoglobin released by lysis of red cells in order to preserve body iron and protein stores.

A

Haptoglobin

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
42
Q

The following are true of complement EXCEPT:

A. Both C3 and C4 are now easily quantitated by nephelometry for monitoring rheumatic disease activity.

B. C3 and also C4 concentration is a convenient marker for assessing such as lupus erythematosus and rheumatoid arthritis.

C. C3 is not appreciated on serum protein electrophoresis because its concentration is about one fifth that of C4.

D. C3 is cleaved to form C3c

A

C. C3 is not appreciated on serum protein electrophoresis because its concentration is about one fifth that of C4.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
43
Q

The most abundant coagulation factor.

A. Factor VIII
B. Factor V
C. Fibrinogen
D. Thrombin

A

C. Fibrinogen

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
44
Q

The following is true of fibrinogen EXCEPT:

A. Fibrinogen levels also rise with pregnancy and use of contraceptive medications.

B. Fibrinogen is detected at low concentrations in normal serum and high concentrations in plasma electrophoresis.

C. Fibrinogen has a molecular weight of 340,000 Da.

D. Both A and B.

A

B. Fibrinogen is detected at low concentrations in normal serum and high concentrations in plasma electrophoresis.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
45
Q

The following is true of Ceruloplasmin EXCEPT:

A. Exhibits ferroxidase activity that is important in iron metabolism.

B. Ceruloplasmin is at lower levels during birth.

C. Can bind 4 atoms of copper.

D. Ceruloplasmin is synthesized in the liver.

A

C. Can bind 4 atoms of copper.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
46
Q

This protein is important in binding and transporting Vitamin D.

A

Gc-Globulin

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
47
Q

The following is true of alpha1-Acid Glycoprotein EXCEPT:

A. Contains a very high carbohydrate content

B. Also known as orosomucoid

C. Negative phase reactant

D. May be important in the transport and metabolism of progesterone.

A

C. Negative phase reactant

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
48
Q

Serves as a general scavenger molecule.

A

C-Reactive Proteins

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
49
Q

Which is true of C-Reactive Proteins?

A. Lowest normal plasma concentration is during adulthood.

B. It is negative phase reactant.

C. Used to monitor autoimmune disease.

D. CRP is an alpha-migrating protein that may form a minor bur distinct monoclonal-appearing band in patients having severe inflammatory response.

A

C. Used to monitor autoimmune disease.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
50
Q

Highly specific for neutralizing chymotrypsin which cleaves peptide bonds at the carboxyl side of tyrosine and phenylalanine residues.

A. IATI
B. AAT
C. ATT
D. AAC

A

D. AAC

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
51
Q

Prevents autodigestion of tissues by endogenous cellular enzymes.

A. IATI
B. AAT
C. ATT
D. AAC

A

A. IATI

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
52
Q

Role is to neutralize thrombin.

A. IATI
B. AAT
C. ATT
D. AAC

A

B. AAT

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
53
Q

The following are correctly paired EXCEPT:

A. Diffuse or homogenous: evenly stains nuclei

B. Speckled: stains the edge of the nuclei

C. Nucleolar: stains two or three large fluorescent area

D. Centromere: discrete speckled pattern

A

B. Speckled: stains the edge of the nuclei

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
54
Q

The following statements are true EXCEPT:

A. The Kjedahl Technique is highly accurate but not commonly used in the lab.

B. Protein in a solution absorbs ultraviolet light at 280 nm.

C. In Cation Exchange Chromatography, the positively charged cations are shown while the negative anions are kept.

D. Turbidimetric methods could also be used to detect nucleic acids.

A

C. In Cation Exchange Chromatography, the positively charged cations are shown while the negative anions are kept.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
55
Q

Used to estimate specific gravity and inference protein as well.

A. Kjedahl Technique
B. Copper Sulfate Technique
C. Absorbance
D. Capillary Electrophoresis

A

B. Copper Sulfate Technique

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
56
Q

In turbidimetric method, protein forms a precipitate on the addition of which substance?

A. Ammonia
B. Trichloroacetic acid
C. Copper sulfate
D. Nitrogen

A

B. Trichloroacetic acid

Note: Sulfosalycilic acid can also be used.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
57
Q

Highly specific for proteins and peptide.

A. Colorimetric Method
B. Ion-Exchange Chromatography
C. Turbidimetric Method
D. Affinity Chromatography

A

A. Colorimetric Method

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
58
Q

Used for consistently accurate determination of protein concentration.

A. Colorimetric method
B. Lowry Assay
C. Kjedahl Technique
D. Gl Filtration

A

B. Lowry Assay

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
59
Q

The following are standard dyes for electrophoresis EXCEPT:

A. Ponceau S
B. Amido Black
C. Bromphenol Blue
D. Coomasie Brilliant Blue

A

C. Bromphenol Blue

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
60
Q

Immunologic method that detects proteins in lower concentrations.

A

Radioimmunoassay (RIA) and Enzyme-linked Immunoabsorbent Assay (ELISA)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
61
Q

Detects turbidity produced by precipitation of a reagent antibody with its target protein and is used for measuring major proteins automated immunochemical analyzers.

A

Nephelometry

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
62
Q

Also known as thyroxine-binding prealbumin (TBPA) or transthyretin (TTR).

A. Ceruloplasmin
B. Albumin
C. Prealbumin
D. AAT

A

C. Prealbumin

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
63
Q

Prealbumin plays a significant role in the metabolism of this essential vitamin.

A. Vitamin C
B. Vitamin A
C. Vitamin B12
D. Vitamin D

A

B. Vitamin A

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
64
Q

The complex of retinol, RBP, and transthyretin appears to be assembled in the _____________ of hepatocytes.

A. Mitochondria
B. Golgi Apparatus
C. Endoplasmic Reticulum
D. Nucleus

A

C. Endoplasmic Reticulum

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
65
Q

The following are true of prealbumin EXCEPT:

A. B-pleated sheet conformation
B. Rich in lysine
C. Half-life of roughly 2 days
D. A better early indicator of change in nutritional status than other commonly used markers

A

B. Rich in lysine

Prealbumin is rich in tryptophan.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
66
Q

TRUE or FALSE: True prealbumin is generally below the level of detection by serum electrophoresis, it is best quantified by immunologic measurements as nephelometry.

A

TRUE

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
67
Q

Portion of prealbumin is the source of _____________ component in type I familial amyloidotic polyneuropathy.

A

B-fibrillar amyloid

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
68
Q

Most abundant protein in the plasma constituting up to two thirds of total plasma protein.

A. Ceruloplasmin
B. Albumin
C. Prealbumin
D. Fibrinogen

A

B. Albumin

69
Q

Depressions of albumin result in serious imbalance of intravascular oncotic pressure. This loss is manifested clinically by the development of __________________.

A

Peripheral edema

70
Q

The following are true of albumin EXCEPT:

A. Congenital absence of albumin generally does not lead to such problems.

B. One of its function is to serve as a mobile repository of amino acids for incorporation into other proteins.

C. The primary sequence of albumin contains five major regions with five peptide loops each.

D. Up to 8% of albumin circulating in normal persons become glycosylated nonenzymatically.

A

C. The primary sequence of albumin contains five major regions with five peptide loops each.

71
Q

Half life of circulating albumin.

A. ~2 days
B. ~10 days
C. ~17 days
D. ~50 days

A

C. ~17 days

72
Q

Most abundant alpha globulin and most important protease inhibitor in plasma.

A

Alpha-1 Antitrypsin (AAT)

73
Q

AAT is is coded by SERPINA1 on _____________.

A. Chromosome 15
B. Chromosome 20
C. Chromosome 14
D. Chromosome 9

A

C. Chromosome 14

74
Q

AAT phenotype susceptible for pulmonary or hepatic disease.

A. SZ phenotype
B. SS homozygous
C. ZZ homozygous
D. MZ phenotype

A

C. ZZ homozygous

75
Q

The following are true of the phenotypes for AAT EXCEPT:

A. PiS variant of alele are moderately reduced AAT expression but without any disease associated.

B. Phenotype MZ exhibits with reduced AAT.

C. Majority are Z alele or phenotype ZZ.

D. SS and SZ phenotype suspected of increased lung and hepatic disease.

A

C. Majority are Z alele or phenotype ZZ.

76
Q

Largest major nonimmunoglobulin protein plasma.

A. Alpha-2 macroglobulin
B. Fibrinogen
C. Ceruloplasmin
D. Prealbumin

A

A. Alpha-2 macroglobulin

77
Q

The following are true of alpha-2 macroglobulin EXCEPT:

A. Women have higher levels than men in response to estrogen.

B. Inactivates proteases by complexing with them and forming covalent bonds to them.

C. Combines with hemoglobin released by lysis of red cells in order ro preserve body iron and protein stores.

D. The spectrum of inhibition by AMG is very wide.

A

C. Combines with hemoglobin released by lysis of red cells in order ro preserve body iron and protein stores.

78
Q

The following statements are true EXCEPT:

A. The Kjedahl Technique is highly accurate but not commonly used in the lab.

B. Protein in a solution absorbs ultraviolet light at 280 nm.

C. In Cation Exchange Chromatography, the positively charged cations are shown while the negative anions are kept.

D. Turbidimetric methods could also be used to detect nucleic acids.

A

C. In Cation Exchange Chromatography, the positively charged cations are shown while the negative anions are kept.

79
Q

Used to estimate specific gravity and inference protein as well.

A. Kjedahl Technique
B. Copper Sulfate Technique
C. Absorbance
D. Capillary Electrophoresis

A

B. Copper Sulfate Technique

80
Q

In turbidimetric method, protein forms a precipitate on the addition of which substance?

A. Ammonia
B. Trichloroacetic acid
C. Copper sulfate
D. Nitrogen

A

B. Trichloroacetic acid

Note: Sulfosalycilic acid can also be used.

81
Q

Highly specific for proteins and peptide.

A. Colorimetric Method
B. Ion-Exchange Chromatography
C. Turbidimetric Method
D. Affinity Chromatography

A

A. Colorimetric Method

82
Q

Used for consistently accurate determination of protein concentration.

A. Colorimetric method
B. Lowry Assay
C. Kjedahl Technique
D. Gl Filtration

A

B. Lowry Assay

83
Q

The following are standard dyes for electrophoresis EXCEPT:

A. Ponceau S
B. Amido Black
C. Bromphenol Blue
D. Coomasie Brilliant Blue

A

C. Bromphenol Blue

84
Q

Immunologic method that detects proteins in lower concentrations.

A

Radioimmunoassay (RIA) and Enzyme-linked Immunoabsorbent Assay (ELISA)

85
Q

Detects turbidity produced by precipitation of a reagent antibody with its target protein and is used for measuring major proteins automated immunochemical analyzers.

A

Nephelometry

86
Q

Also known as thyroxine-binding prealbumin (TBPA) or transthyretin (TTR).

A. Ceruloplasmin
B. Albumin
C. Prealbumin
D. AAT

A

C. Prealbumin

87
Q

Prealbumin plays a significant role in the metabolism of this essential vitamin.

A. Vitamin C
B. Vitamin A
C. Vitamin B12
D. Vitamin D

A

B. Vitamin A

88
Q

The complex of retinol, RBP, and transthyretin appears to be assembled in the _____________ of hepatocytes.

A. Mitochondria
B. Golgi Apparatus
C. Endoplasmic Reticulum
D. Nucleus

A

C. Endoplasmic Reticulum

89
Q

The following are true of prealbumin EXCEPT:

A. B-pleated sheet conformation
B. Rich in lysine
C. Half-life of roughly 2 days
D. A better early indicator of change in nutritional status than other commonly used markers

A

B. Rich in lysine

Prealbumin is rich in tryptophan.

90
Q

TRUE or FALSE: True prealbumin is generally below the level of detection by serum electrophoresis, it is best quantified by immunologic measurements as nephelometry.

A

TRUE

91
Q

Portion of prealbumin is the source of _____________ component in type I familial amyloidotic polyneuropathy.

A

B-fibrillar amyloid

92
Q

Most abundant protein in the plasma constituting up to two thirds of total plasma protein.

A. Ceruloplasmin
B. Albumin
C. Prealbumin
D. Fibrinogen

A

B. Albumin

93
Q

Depressions of albumin result in serious imbalance of intravascular oncotic pressure. This loss is manifested clinically by the development of __________________.

A

Peripheral edema

94
Q

The following are true of albumin EXCEPT:

A. Congenital absence of albumin generally does not lead to such problems.

B. One of its function is to serve as a mobile repository of amino acids for incorporation into other proteins.

C. The primary sequence of albumin contains five major regions with five peptide loops each.

D. Up to 8% of albumin circulating in normal persons become glycosylated nonenzymatically.

A

C. The primary sequence of albumin contains five major regions with five peptide loops each.

95
Q

Half life of circulating albumin.

A. ~2 days
B. ~10 days
C. ~17 days
D. ~50 days

A

C. ~17 days

96
Q

Most abundant alpha globulin and most important protease inhibitor in plasma.

A

Alpha-1 Antitrypsin (AAT)

97
Q

AAT is is coded by SERPINA1 on _____________.

A. Chromosome 15
B. Chromosome 20
C. Chromosome 14
D. Chromosome 9

A

C. Chromosome 14

98
Q

AAT phenotype susceptible for pulmonary or hepatic disease.

A. SZ phenotype
B. SS homozygous
C. ZZ homozygous
D. MZ phenotype

A

C. ZZ homozygous

99
Q

The following are true of the phenotypes for AAT EXCEPT:

A. PiS variant of alele are moderately reduced AAT expression but without any disease associated.

B. Phenotype MZ exhibits with reduced AAT.

C. Majority are Z alele or phenotype ZZ.

D. SS and SZ phenotype suspected of increased lung and hepatic disease.

A

C. Majority are Z alele or phenotype ZZ.

100
Q

Largest major nonimmunoglobulin protein plasma.

A. Alpha-2 macroglobulin
B. Fibrinogen
C. Ceruloplasmin
D. Prealbumin

A

A. Alpha-2 macroglobulin

101
Q

The following are true of alpha-2 macroglobulin EXCEPT:

A. Women have higher levels than men in response to estrogen.

B. Inactivates proteases by complexing with them and forming covalent bonds to them.

C. Combines with hemoglobin released by lysis of red cells in order ro preserve body iron and protein stores.

D. The spectrum of inhibition by AMG is very wide.

A

C. Combines with hemoglobin released by lysis of red cells in order ro preserve body iron and protein stores.

102
Q

The following statements are true EXCEPT:

A. The Kjedahl Technique is highly accurate but not commonly used in the lab.

B. Protein in a solution absorbs ultraviolet light at 280 nm.

C. In Cation Exchange Chromatography, the positively charged cations are shown while the negative anions are kept.

D. Turbidimetric methods could also be used to detect nucleic acids.

A

C. In Cation Exchange Chromatography, the positively charged cations are shown while the negative anions are kept.

103
Q

Used to estimate specific gravity and inference protein as well.

A. Kjedahl Technique
B. Copper Sulfate Technique
C. Absorbance
D. Capillary Electrophoresis

A

B. Copper Sulfate Technique

104
Q

In turbidimetric method, protein forms a precipitate on the addition of which substance?

A. Ammonia
B. Trichloroacetic acid
C. Copper sulfate
D. Nitrogen

A

B. Trichloroacetic acid

Note: Sulfosalycilic acid can also be used.

105
Q

Highly specific for proteins and peptide.

A. Colorimetric Method
B. Ion-Exchange Chromatography
C. Turbidimetric Method
D. Affinity Chromatography

A

A. Colorimetric Method

106
Q

Used for consistently accurate determination of protein concentration.

A. Colorimetric method
B. Lowry Assay
C. Kjedahl Technique
D. Gl Filtration

A

B. Lowry Assay

107
Q

The following are standard dyes for electrophoresis EXCEPT:

A. Ponceau S
B. Amido Black
C. Bromphenol Blue
D. Coomasie Brilliant Blue

A

C. Bromphenol Blue

108
Q

Immunologic method that detects proteins in lower concentrations.

A

Radioimmunoassay (RIA) and Enzyme-linked Immunoabsorbent Assay (ELISA)

109
Q

Detects turbidity produced by precipitation of a reagent antibody with its target protein and is used for measuring major proteins automated immunochemical analyzers.

A

Nephelometry

110
Q

Also known as thyroxine-binding prealbumin (TBPA) or transthyretin (TTR).

A. Ceruloplasmin
B. Albumin
C. Prealbumin
D. AAT

A

C. Prealbumin

111
Q

Prealbumin plays a significant role in the metabolism of this essential vitamin.

A. Vitamin C
B. Vitamin A
C. Vitamin B12
D. Vitamin D

A

B. Vitamin A

112
Q

The complex of retinol, RBP, and transthyretin appears to be assembled in the _____________ of hepatocytes.

A. Mitochondria
B. Golgi Apparatus
C. Endoplasmic Reticulum
D. Nucleus

A

C. Endoplasmic Reticulum

113
Q

The following are true of prealbumin EXCEPT:

A. B-pleated sheet conformation
B. Rich in lysine
C. Half-life of roughly 2 days
D. A better early indicator of change in nutritional status than other commonly used markers

A

B. Rich in lysine

Prealbumin is rich in tryptophan.

114
Q

TRUE or FALSE: True prealbumin is generally below the level of detection by serum electrophoresis, it is best quantified by immunologic measurements as nephelometry.

A

TRUE

115
Q

Portion of prealbumin is the source of _____________ component in type I familial amyloidotic polyneuropathy.

A

B-fibrillar amyloid

116
Q

Most abundant protein in the plasma constituting up to two thirds of total plasma protein.

A. Ceruloplasmin
B. Albumin
C. Prealbumin
D. Fibrinogen

A

B. Albumin

117
Q

Depressions of albumin result in serious imbalance of intravascular oncotic pressure. This loss is manifested clinically by the development of __________________.

A

Peripheral edema

118
Q

The following are true of albumin EXCEPT:

A. Congenital absence of albumin generally does not lead to such problems.

B. One of its function is to serve as a mobile repository of amino acids for incorporation into other proteins.

C. The primary sequence of albumin contains five major regions with five peptide loops each.

D. Up to 8% of albumin circulating in normal persons become glycosylated nonenzymatically.

A

C. The primary sequence of albumin contains five major regions with five peptide loops each.

119
Q

Half life of circulating albumin.

A. ~2 days
B. ~10 days
C. ~17 days
D. ~50 days

A

C. ~17 days

120
Q

Most abundant alpha globulin and most important protease inhibitor in plasma.

A

Alpha-1 Antitrypsin (AAT)

121
Q

AAT is is coded by SERPINA1 on _____________.

A. Chromosome 15
B. Chromosome 20
C. Chromosome 14
D. Chromosome 9

A

C. Chromosome 14

122
Q

AAT phenotype susceptible for pulmonary or hepatic disease.

A. SZ phenotype
B. SS homozygous
C. ZZ homozygous
D. MZ phenotype

A

C. ZZ homozygous

123
Q

The following are true of the phenotypes for AAT EXCEPT:

A. PiS variant of alele are moderately reduced AAT expression but without any disease associated.

B. Phenotype MZ exhibits with reduced AAT.

C. Majority are Z alele or phenotype ZZ.

D. SS and SZ phenotype suspected of increased lung and hepatic disease.

A

C. Majority are Z alele or phenotype ZZ.

124
Q

Largest major nonimmunoglobulin protein plasma.

A. Alpha-2 macroglobulin
B. Fibrinogen
C. Ceruloplasmin
D. Prealbumin

A

A. Alpha-2 macroglobulin

125
Q

The following are true of alpha-2 macroglobulin EXCEPT:

A. Women have higher levels than men in response to estrogen.

B. Inactivates proteases by complexing with them and forming covalent bonds to them.

C. Combines with hemoglobin released by lysis of red cells in order ro preserve body iron and protein stores.

D. The spectrum of inhibition by AMG is very wide.

A

C. Combines with hemoglobin released by lysis of red cells in order ro preserve body iron and protein stores.

126
Q

The following statements are true EXCEPT:

A. The Kjedahl Technique is highly accurate but not commonly used in the lab.

B. Protein in a solution absorbs ultraviolet light at 280 nm.

C. In Cation Exchange Chromatography, the positively charged cations are shown while the negative anions are kept.

D. Turbidimetric methods could also be used to detect nucleic acids.

A

C. In Cation Exchange Chromatography, the positively charged cations are shown while the negative anions are kept.

127
Q

Used to estimate specific gravity and inference protein as well.

A. Kjedahl Technique
B. Copper Sulfate Technique
C. Absorbance
D. Capillary Electrophoresis

A

B. Copper Sulfate Technique

128
Q

In turbidimetric method, protein forms a precipitate on the addition of which substance?

A. Ammonia
B. Trichloroacetic acid
C. Copper sulfate
D. Nitrogen

A

B. Trichloroacetic acid

Note: Sulfosalycilic acid can also be used.

129
Q

Highly specific for proteins and peptide.

A. Colorimetric Method
B. Ion-Exchange Chromatography
C. Turbidimetric Method
D. Affinity Chromatography

A

A. Colorimetric Method

130
Q

Used for consistently accurate determination of protein concentration.

A. Colorimetric method
B. Lowry Assay
C. Kjedahl Technique
D. Gl Filtration

A

B. Lowry Assay

131
Q

The following are standard dyes for electrophoresis EXCEPT:

A. Ponceau S
B. Amido Black
C. Bromphenol Blue
D. Coomasie Brilliant Blue

A

C. Bromphenol Blue

132
Q

Immunologic method that detects proteins in lower concentrations.

A

Radioimmunoassay (RIA) and Enzyme-linked Immunoabsorbent Assay (ELISA)

133
Q

Detects turbidity produced by precipitation of a reagent antibody with its target protein and is used for measuring major proteins automated immunochemical analyzers.

A

Nephelometry

134
Q

Also known as thyroxine-binding prealbumin (TBPA) or transthyretin (TTR).

A. Ceruloplasmin
B. Albumin
C. Prealbumin
D. AAT

A

C. Prealbumin

135
Q

Prealbumin plays a significant role in the metabolism of this essential vitamin.

A. Vitamin C
B. Vitamin A
C. Vitamin B12
D. Vitamin D

A

B. Vitamin A

136
Q

The complex of retinol, RBP, and transthyretin appears to be assembled in the _____________ of hepatocytes.

A. Mitochondria
B. Golgi Apparatus
C. Endoplasmic Reticulum
D. Nucleus

A

C. Endoplasmic Reticulum

137
Q

The following are true of prealbumin EXCEPT:

A. B-pleated sheet conformation
B. Rich in lysine
C. Half-life of roughly 2 days
D. A better early indicator of change in nutritional status than other commonly used markers

A

B. Rich in lysine

Prealbumin is rich in tryptophan.

138
Q

TRUE or FALSE: True prealbumin is generally below the level of detection by serum electrophoresis, it is best quantified by immunologic measurements as nephelometry.

A

TRUE

139
Q

Portion of prealbumin is the source of _____________ component in type I familial amyloidotic polyneuropathy.

A

B-fibrillar amyloid

140
Q

Most abundant protein in the plasma constituting up to two thirds of total plasma protein.

A. Ceruloplasmin
B. Albumin
C. Prealbumin
D. Fibrinogen

A

B. Albumin

141
Q

The following statements are true EXCEPT:

A. The Kjedahl Technique is highly accurate but not commonly used in the lab.

B. Protein in a solution absorbs ultraviolet light at 280 nm.

C. In Cation Exchange Chromatography, the positively charged cations are shown while the negative anions are kept.

D. Turbidimetric methods could also be used to detect nucleic acids.

A

C. In Cation Exchange Chromatography, the positively charged cations are shown while the negative anions are kept.

142
Q

Used to estimate specific gravity and inference protein as well.

A. Kjedahl Technique
B. Copper Sulfate Technique
C. Absorbance
D. Capillary Electrophoresis

A

B. Copper Sulfate Technique

143
Q

In turbidimetric method, protein forms a precipitate on the addition of which substance?

A. Ammonia
B. Trichloroacetic acid
C. Copper sulfate
D. Nitrogen

A

B. Trichloroacetic acid

Note: Sulfosalycilic acid can also be used.

144
Q

Highly specific for proteins and peptide.

A. Colorimetric Method
B. Ion-Exchange Chromatography
C. Turbidimetric Method
D. Affinity Chromatography

A

A. Colorimetric Method

145
Q

Used for consistently accurate determination of protein concentration.

A. Colorimetric method
B. Lowry Assay
C. Kjedahl Technique
D. Gl Filtration

A

B. Lowry Assay

146
Q

The following are standard dyes for electrophoresis EXCEPT:

A. Ponceau S
B. Amido Black
C. Bromphenol Blue
D. Coomasie Brilliant Blue

A

C. Bromphenol Blue

147
Q

Immunologic method that detects proteins in lower concentrations.

A

Radioimmunoassay (RIA) and Enzyme-linked Immunoabsorbent Assay (ELISA)

148
Q

Detects turbidity produced by precipitation of a reagent antibody with its target protein and is used for measuring major proteins automated immunochemical analyzers.

A

Nephelometry

149
Q

Also known as thyroxine-binding prealbumin (TBPA) or transthyretin (TTR).

A. Ceruloplasmin
B. Albumin
C. Prealbumin
D. AAT

A

C. Prealbumin

150
Q

Prealbumin plays a significant role in the metabolism of this essential vitamin.

A. Vitamin C
B. Vitamin A
C. Vitamin B12
D. Vitamin D

A

B. Vitamin A

151
Q

The complex of retinol, RBP, and transthyretin appears to be assembled in the _____________ of hepatocytes.

A. Mitochondria
B. Golgi Apparatus
C. Endoplasmic Reticulum
D. Nucleus

A

C. Endoplasmic Reticulum

152
Q

The following are true of prealbumin EXCEPT:

A. B-pleated sheet conformation
B. Rich in lysine
C. Half-life of roughly 2 days
D. A better early indicator of change in nutritional status than other commonly used markers

A

B. Rich in lysine

Prealbumin is rich in tryptophan.

153
Q

TRUE or FALSE: True prealbumin is generally below the level of detection by serum electrophoresis, it is best quantified by immunologic measurements as nephelometry.

A

TRUE

154
Q

Portion of prealbumin is the source of _____________ component in type I familial amyloidotic polyneuropathy.

A

B-fibrillar amyloid

155
Q

Most abundant protein in the plasma constituting up to two thirds of total plasma protein.

A. Ceruloplasmin
B. Albumin
C. Prealbumin
D. Fibrinogen

A

B. Albumin

156
Q

The following statements are true EXCEPT:

A. The Kjedahl Technique is highly accurate but not commonly used in the lab.

B. Protein in a solution absorbs ultraviolet light at 280 nm.

C. In Cation Exchange Chromatography, the positively charged cations are shown while the negative anions are kept.

D. Turbidimetric methods could also be used to detect nucleic acids.

A

C. In Cation Exchange Chromatography, the positively charged cations are shown while the negative anions are kept.

157
Q

Used to estimate specific gravity and inference protein as well.

A. Kjedahl Technique
B. Copper Sulfate Technique
C. Absorbance
D. Capillary Electrophoresis

A

B. Copper Sulfate Technique

158
Q

In turbidimetric method, protein forms a precipitate on the addition of which substance?

A. Ammonia
B. Trichloroacetic acid
C. Copper sulfate
D. Nitrogen

A

B. Trichloroacetic acid

Note: Sulfosalycilic acid can also be used.

159
Q

Highly specific for proteins and peptide.

A. Colorimetric Method
B. Ion-Exchange Chromatography
C. Turbidimetric Method
D. Affinity Chromatography

A

A. Colorimetric Method

160
Q

Used for consistently accurate determination of protein concentration.

A. Colorimetric method
B. Lowry Assay
C. Kjedahl Technique
D. Gl Filtration

A

B. Lowry Assay

161
Q

The following are standard dyes for electrophoresis EXCEPT:

A. Ponceau S
B. Amido Black
C. Bromphenol Blue
D. Coomasie Brilliant Blue

A

C. Bromphenol Blue

162
Q

Immunologic method that detects proteins in lower concentrations.

A

Radioimmunoassay (RIA) and Enzyme-linked Immunoabsorbent Assay (ELISA)

163
Q

Detects turbidity produced by precipitation of a reagent antibody with its target protein and is used for measuring major proteins automated immunochemical analyzers.

A

Nephelometry

164
Q

Also known as thyroxine-binding prealbumin (TBPA) or transthyretin (TTR).

A. Ceruloplasmin
B. Albumin
C. Prealbumin
D. AAT

A

C. Prealbumin

165
Q

Prealbumin plays a significant role in the metabolism of this essential vitamin.

A. Vitamin C
B. Vitamin A
C. Vitamin B12
D. Vitamin D

A

B. Vitamin A

166
Q

The complex of retinol, RBP, and transthyretin appears to be assembled in the _____________ of hepatocytes.

A. Mitochondria
B. Golgi Apparatus
C. Endoplasmic Reticulum
D. Nucleus

A

C. Endoplasmic Reticulum

167
Q

The following are true of prealbumin EXCEPT:

A. B-pleated sheet conformation
B. Rich in lysine
C. Half-life of roughly 2 days
D. A better early indicator of change in nutritional status than other commonly used markers

A

B. Rich in lysine

Prealbumin is rich in tryptophan.

168
Q

TRUE or FALSE: True prealbumin is generally below the level of detection by serum electrophoresis, it is best quantified by immunologic measurements as nephelometry.

A

TRUE

169
Q

Portion of prealbumin is the source of _____________ component in type I familial amyloidotic polyneuropathy.

A

B-fibrillar amyloid

170
Q

Most abundant protein in the plasma constituting up to two thirds of total plasma protein.

A. Ceruloplasmin
B. Albumin
C. Prealbumin
D. Fibrinogen

A

B. Albumin

LABORATORY DIAGNOSIS (1 decks)

Brainscape helps you reach your goals faster, through stronger study habits.
© 2024 Bold Learning Solutions. Terms and Conditions