Module 1 Flashcards

1
Q

connections between eukaryotes and prokaryotes are supported by the ___ theory

A

endosymbiont

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2
Q

what is the endosymbiont hypothesis

A

Mitochondria and chloroplasts evolved from free-living bacteria via symbiosis with a eukaryotic host cell
○ These organelles contain their own DNA
○ Have a distinct translation system

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3
Q

name 4 things that are present in a eukaryotic cell and not a prokaryotic cell

A

membrane bound nucleus (prokaryotes have nucleiod)

lysosomes/peroxisomes

endoplasmic recticulum/golgi apparatus

mitochondria

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4
Q

what are the two ensosymbiont hypotheses and their differences

A

Archezoan scenario
-The mitochondrial endosymbiosis (the engulfment of an alpha-proteobacterium that became mitochondria) occurred AFTER the development of the basic eukaryotic cell structure.

Symbiogenesis scenario
-proposes that the origin of mitochondria and the origin of the eukaryotic cell were closely linked, and the two events happened together in a symbiotic relationship.

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5
Q

what does the cell wall of bacteria have that archaea and eukaryota do not have

A

murein/peptidoglycan

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6
Q

what does the web of life emphasize

A

horizontal gene transfer

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7
Q

which of the domains of life are viruses capable of interacting with

A

all of them

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8
Q

when not inside of a host, viruses exist as ___ with _____

A

virions, genetic material/capsit/envelope

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9
Q

viruses rely on

A

host machinery to replicate and then leave to infect new cells

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10
Q

viral replication strategies

A

carries genetic info as DNA or RNA and uses host machinery to transcribe it to make progeny

genome integration into host genome

when it leaves cell (lysogeny) it can take host genome with it to new cells - this can be between species

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11
Q

discuss the hypotheses around the origins of viruses

A

virus first theory
-viruses came before cells
-evolved from a mixture of macromolecules and existed as self replicating units
-self replicating units of RNA that were first in existence may have gained the ability to infect earlier forms of cells (can catalyze chemical reactions using enzymatic like properties

escape theory
-cells came before viruses
viruses come from bits of cellular RNA/DNA that leaked/escaped from cells
-these parts acquired protein coats allowing them to be independent entities - can interact with cells
-retrotransposons (resemble these)

Reduction theory
-cells came before viruses
-viruses come from small primordial cells that lost cellular components over the course of evolution
-maintained genetic material and elements for replication
-infected other cells and now dependent on host cell as they lost their cellular elements

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12
Q

evidence supporting that mitochondria evolved from bacteria

A

-double membrane (like gram-)
-circular DNA
-Phylogenetic relationships
-binary fission for reproduction
-similar proteins of ribosomes compared to eukaryotic ribosomes
-endosymbiotic relationships

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13
Q

what are some factors to explain the diversity of the microbial world

A

rapid reproduction

HGT and VGT

varied habitats/selective pressures

metabolic diversity (photosynthesis and the metabolism of sulfur or nitrogen)

evolutionary history

interactions with other organisms

mutations

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14
Q

The 4 phyla of bacteria that dominate the human body

A

Actinobacteria
○ Gram-positive bacteria that are aerobic and mostly found in soil

Bacteroidetes
○ Gram-negative phylum which have colonized virtually all types of habitats on Earth
○ Bacteroides
§ Predominant in the gut

Firmicutes
○ Gram-positive and can often form endospores
○ Streptococcus predominant in the mouth

Proteobacteria
○ Major Gram-negative phylum includes a diverse array of bacteria

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15
Q

Gene transfer in bactera

A

conjugation
- direct, via a pilus

transformation
-uptake from environment

transduction
-from viral infefction

transposition
-jumping from one chromosomal location to another

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15
Q

pathogenic vs commensal bacteria

A

Pathogenic Bacteria
○ Ability to cause infection
○ Some are opportunistic
§ Take advantage of an opportunity that is not usually available to infect another organism

Commensal Bacteria
○ Non-pathogenic
○ Have a symbiotic relationship with the host

16
Q

how does HGT and selective pressure lead to antibiotic resistance

A

HGT: uptake of genetic information from bacteria that have survived an antibiotic attack

rapidly spread resistance

make specific resistance

selective pressures can lead to multiple ways to resist - polyclonal resistance and can then avoid many versions of attack

17
Q

mechanisms of resistance of bacteria to antibiotics

A

Target protection

Target modification

Prevention of intracellular antibiotic accumulation
- efflux pump

antibiotic detoxification
-degrading enzymes

18
Q

how does antibiotic use facilitate resistance

A

long term use

inappropriate use

available without consultation - too accessible

inappropriate prescriptions (such as for viral infection), use in livestock and poultry