Module 01 - Introduction to Microbial Cells Flashcards
Define “spontaneous generation”
life can arise from nonliving matter
How did Francesco Redi test spontaneous generation?
- performed an experiment on the notion of spontaneous generation
- refute the idea of spontaneous generation that maggots can spontaneously generate from meat left in open air
- 6 containers - 2 open to air, 2 covered with gauze, and 2 completely closed
- maggots formed only to the jar that was left completely open, this was because of the presence of flies on the meat, causing maggots to form
How did John Needham test spontaneous generation?
- boiled broth of animal and plant matter to kill preexsiting microbes
- broth became cloudy (sealed jars)
- microbes were still in the broth
- argued that the microbes had arised spontaneously
- reality: he didnt boil the broth enough to kill the microbes
How did Lazzaro Spallanzani test spontaneous generation?
- didn’t agree with Needhams results
- performed same experiment as Needham but with many different jars
- used sealed and unsealed jars
- heated but sealed flasks clear with no sign of spontaneous generation, unless the jars were left open
- microbes were introduced to the jars from the air
Microbe
a microorganism, especially a bacterium causing disease or fermentation.
Louis Pasteur - spontaneous generation
- flasks were able to exchange air from outside
- bends and twists in the flask wouldn’t allow for air to come in, unless broken
- airborne microorganisms allow microbes to grow in the broth
- “life is a germ and germ is life”
- boiling the broth kills the microorganisms
Cell Theory
is a scientific theory first formulated in the mid-nineteenth century, that living organisms are made up of cells, that they are the basic structural/organizational unit of all organisms, and that all cells come from pre-existing cells
Two basic tenets of cell theory
- all cells only come from other cells (principle of biogensis)
- cells are a fundamental units of organisms
Endosymbiotic Theory
is the leading evolutionary theory of the origin of eukaryotic cells from prokaryotic organisms (symbiogenesis)
- defined as the theory that mitochondria and chloroplasts arose as a result of prokaryotic cells establishing a symbiotic relationship within a eukaryotic host
Germ theory of disease
diseased may result from microbial infection
Distinguish characteristics of prokaryotic cells
- smaller than eukaryotic cells
- have no nucleus
- lack organelles
- singular, circular chromosome in the nucleiod
- classified as Archaea and Bacteria
- lack membrane bound organelles, inclusions to compartmentalize for cytoplasm
Organelles in prokaryotic cells
plasma membrane, cell wall, capsule, cytoplasm, ribosome, nucleiod, inclusion, plasmid, pilus, and flagellum
Cell Wall
envelopes the cell membrane protecting the cell from osmotic pressure
protects cell from harsh conditions in outside environment, main component of cell wall - peptidoglycan
isotonic membrane
solute concentration inside and outside of cell are equal, not net movement of water
hypertonic medium
solute concentration outside cell is more than inside, water diffuses out of cell
hypotonic medium
solute concentration inside cell is higher than outside, water moves by osmosis into cell (cell can swell and burst)
Crenation
cell can become dehydrated in hypertonic environment
plasmolysis
(cell with cell wall) plasma membrane contracts and detaches from cell wall for shape
Nucleoid
prokaryotic DNA and DNA proteins, DNA interacts with nucleiod - associated proteins (NAPs)
plasmids
extrachromosomal DNA found, plasmids found in bacteria
ribosomes
constructed of proteins and rRNA, found in cytoplasm
inclusion
store excess nutrients in cytoplasmic structures (ex. glycogen, starches and polyhydroxybutyhate)
endospores
bacteria cells are observed as vegetative cells, bacteria can form endospores
a dormant, tough, and non-reproductive structure produced by certain bacteria
plasma membrane
eukaryotic and prokaryotic cells have a plasma membrane
he membrane found in all cells that separates the interior of the cell from the outside environment
flagella
structures to move in aqueous environment
eukaryotic cells
are organisms whose cells have a nucleus enclosed within a nuclear envelope. They belong to the group of organisms Eukaryota or Eukarya
nucleus
complex nuclear membrane that houses the DNA genome
nucleolous
region in the nucleus where RNA occurs, assembly of ribosomes occur