MODULE 0 AND 1 Flashcards
lens, usually 10x in magnification, where you look through in the microscope
ocular/eyepiece
revolving nosepiece
where the objectives are attached
arm
where the microscope is grasped
objective lenses
lenses located above the stage
stage
platform under objectves where the specimen is placed
stage adjustment knobs
moves the slide left or right and forward or backward
lamp
built in light source for electric microscope
aperture diaphragm control
regulates the amount of light reaching the specimen
condenser
lens located below the stage which collects light and delivers it to the objective
coarse focus adjustment
focuses the specimen under the scanner and LPO
fine focus adjustment
focuses the specimen under hpo and oio
three basic parts of a microscope
mechanical, magnifying, illuminating
these are under illuminatio
lamp, condenser, iris diaphragm
under magnification
objectives and eyepiece
under mechanical
stage, coarse and fine knobs, revolving nosepiece, and stage adjustment knobs and slide holder
the shape of bacillus subtilis
rod-shaped
domain of neisseria subflava
bacteria
ability of a microscope lens to gather fine details of the specimen being discovered
limit or resolution / resolving power
formula of limit of resolution
abbe’s equation
states that resolution is defined by the wavelength of light and the NA
abbe’s equation
the constant value derived from the properties of light
0.61
wavelength of visible light
lambda
the refractive index of the medium between objective lens and glass slide
eta
half the value of the angular aperture
theta
cone angle; cone of light that enters the objective lens
angular aperture
baker’s yeast
fungi; photosynthetic absent
ciliate
protozoa; absent
chlamydomonas reinhardtii
algae; present
e.coli
bacteria; absent
paramecium caudatum
protozoa; absent
euglena
protozoa; present
meat mold
fungi; absent
spirogyra
algae; present
eukaryotic microbes that comprises the unicellular fungi
yeasts
eukaryotic cells are only multicellular
false
molds
filamentous fungi
filamentous cells of molds
hyphae
masses of hyphae
mycelia
motility structure for amoebae
pseudopodia
these composed the numerical aperture of a microscope
refractive index, angular aperture, working distance
the eta value of air
1.00
eta value of water
1.33
eta value of oil
1.5
the ability to achieve a clear image, typically achieved by moving either the eyepiece tubes or the stage
focus
determined by multiplying the magnification capability of the eyepiece lens by that of the objective lens.
total magnification
The lens closest to the specimen that first receives the rays from the specimen (the object) and forms the image in the focal plane of the eyepiece is the OCULAR.
false; should be objective lens
The platform on which slides and specimens are placed for viewing refers to the STAGE CLIPS.
stage
The COURSE ADJUSTMENT refers to the knob on the side of the microscope that moves the objective lens up and down
true
heterogeneous group of photosynthetic organisms
algae
these are composed of cyanobacteria
prokaryotic algae
diatoms, dinoflagellates, many green algae are examples of
eukaryotic algae
where the specimen being viewed dark against a light background
bright-field microscope
to keep bad bugs away from people
biosafety
to keep bad people away from bugs
biosecurity
the most effective in the hierarchy of controls
elimination
this is the order of hierarchy (from least to most effective
ppe > administrative controls > engineering controls > substitution > elimination
this means removing the hazard
eliminatiion
replacing the hazard
substitution
isolating people from the hazard
engineering controls
changing the way people work
administrative controls
protecting the worker with this
ppe
key considerations in ppe selection
consider potential routes of infection and exposure
consider what the ppe is protecting
consider the limitations for the use of types of ppe
consider how and where ppe should be donned and doffed
these are the personal protection requirements and practices in a microbiology lab (bench top work
- lab gowns
- closed toe shoes
- safety goggles
- gloves
- handwashing
this is the sequence for putting on ppe
gown, mask, goggles, gloves
how to handle laboratory spills
stop what you are doing presume you are contaminated inform others in the area of spill localize the spilled materials label the area as contaminated
the correct waste hierarchy from least to highest
biological > chemical > radioactive
also known as biomedical waste/infectious waste
biological waste
the color coding of waste
black (general waste) green (wet waste) yellow (infectious waste) yellow with black band (chemical and pharmaceutical waste) orange (radioactive waste) sharps collections
these are routes of exposure
contact, inhalation, ingestion, percutaneous
These are under BSL 1
no containment
defined organisms
unlikely to cause disease
ex: e-coli
under bsl 2
containment
moderate risks
varying severity
ex: influenza, hiv, lyme disease
under bsl 3
high containment
aerosol transmission
serious
ex: tuberculosis
under bsl 4
max containment
life threatening
ex: e-bola
the color code of health
blue
color code of flammability
red
color code of reactivity
yellow
