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MICROLAB > MODULE 0 AND 1 > Flashcards

MODULE 0 AND 1 Flashcards

1
Q

lens, usually 10x in magnification, where you look through in the microscope

A

ocular/eyepiece

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2
Q

revolving nosepiece

A

where the objectives are attached

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3
Q

arm

A

where the microscope is grasped

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4
Q

objective lenses

A

lenses located above the stage

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5
Q

stage

A

platform under objectves where the specimen is placed

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6
Q

stage adjustment knobs

A

moves the slide left or right and forward or backward

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7
Q

lamp

A

built in light source for electric microscope

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8
Q

aperture diaphragm control

A

regulates the amount of light reaching the specimen

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9
Q

condenser

A

lens located below the stage which collects light and delivers it to the objective

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10
Q

coarse focus adjustment

A

focuses the specimen under the scanner and LPO

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11
Q

fine focus adjustment

A

focuses the specimen under hpo and oio

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12
Q

three basic parts of a microscope

A

mechanical, magnifying, illuminating

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13
Q

these are under illuminatio

A

lamp, condenser, iris diaphragm

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14
Q

under magnification

A

objectives and eyepiece

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15
Q

under mechanical

A

stage, coarse and fine knobs, revolving nosepiece, and stage adjustment knobs and slide holder

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16
Q

the shape of bacillus subtilis

A

rod-shaped

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17
Q

domain of neisseria subflava

A

bacteria

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18
Q

ability of a microscope lens to gather fine details of the specimen being discovered

A

limit or resolution / resolving power

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19
Q

formula of limit of resolution

A

abbe’s equation

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20
Q

states that resolution is defined by the wavelength of light and the NA

A

abbe’s equation

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21
Q

the constant value derived from the properties of light

A

0.61

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22
Q

wavelength of visible light

A

lambda

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23
Q

the refractive index of the medium between objective lens and glass slide

A

eta

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24
Q

half the value of the angular aperture

A

theta

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25
Q

cone angle; cone of light that enters the objective lens

A

angular aperture

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26
Q

baker’s yeast

A

fungi; photosynthetic absent

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27
Q

ciliate

A

protozoa; absent

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28
Q

chlamydomonas reinhardtii

A

algae; present

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29
Q

e.coli

A

bacteria; absent

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30
Q

paramecium caudatum

A

protozoa; absent

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31
Q

euglena

A

protozoa; present

32
Q

meat mold

A

fungi; absent

33
Q

spirogyra

A

algae; present

34
Q

eukaryotic microbes that comprises the unicellular fungi

A

yeasts

35
Q

eukaryotic cells are only multicellular

A

false

36
Q

molds

A

filamentous fungi

37
Q

filamentous cells of molds

A

hyphae

38
Q

masses of hyphae

A

mycelia

39
Q

motility structure for amoebae

A

pseudopodia

40
Q

these composed the numerical aperture of a microscope

A

refractive index, angular aperture, working distance

41
Q

the eta value of air

A

1.00

42
Q

eta value of water

A

1.33

43
Q

eta value of oil

A

1.5

44
Q

the ability to achieve a clear image, typically achieved by moving either the eyepiece tubes or the stage

A

focus

45
Q

determined by multiplying the magnification capability of the eyepiece lens by that of the objective lens.

A

total magnification

46
Q

The lens closest to the specimen that first receives the rays from the specimen (the object) and forms the image in the focal plane of the eyepiece is the OCULAR.

A

false; should be objective lens

47
Q

The platform on which slides and specimens are placed for viewing refers to the STAGE CLIPS.

A

stage

48
Q

The COURSE ADJUSTMENT refers to the knob on the side of the microscope that moves the objective lens up and down

A

true

49
Q

heterogeneous group of photosynthetic organisms

A

algae

50
Q

these are composed of cyanobacteria

A

prokaryotic algae

51
Q

diatoms, dinoflagellates, many green algae are examples of

A

eukaryotic algae

52
Q

where the specimen being viewed dark against a light background

A

bright-field microscope

53
Q

to keep bad bugs away from people

A

biosafety

54
Q

to keep bad people away from bugs

A

biosecurity

55
Q

the most effective in the hierarchy of controls

A

elimination

56
Q

this is the order of hierarchy (from least to most effective

A

ppe > administrative controls > engineering controls > substitution > elimination

57
Q

this means removing the hazard

A

eliminatiion

58
Q

replacing the hazard

A

substitution

59
Q

isolating people from the hazard

A

engineering controls

60
Q

changing the way people work

A

administrative controls

61
Q

protecting the worker with this

A

ppe

62
Q

key considerations in ppe selection

A

consider potential routes of infection and exposure
consider what the ppe is protecting
consider the limitations for the use of types of ppe
consider how and where ppe should be donned and doffed

63
Q

these are the personal protection requirements and practices in a microbiology lab (bench top work

A
  1. lab gowns
  2. closed toe shoes
  3. safety goggles
  4. gloves
  5. handwashing
64
Q

this is the sequence for putting on ppe

A

gown, mask, goggles, gloves

65
Q

how to handle laboratory spills

A 
stop what you are doing
presume you are contaminated
inform others in the area of spill
localize the spilled materials
label the area as contaminated
66
Q

the correct waste hierarchy from least to highest

A

biological > chemical > radioactive

67
Q

also known as biomedical waste/infectious waste

A

biological waste

68
Q

the color coding of waste

A 
black (general waste)
green (wet waste)
yellow (infectious waste)
yellow with black band (chemical and pharmaceutical waste)
orange (radioactive waste)
sharps collections
69
Q

these are routes of exposure

A

contact, inhalation, ingestion, percutaneous

70
Q

These are under BSL 1

A

no containment
defined organisms
unlikely to cause disease

ex: e-coli

71
Q

under bsl 2

A

containment
moderate risks
varying severity

ex: influenza, hiv, lyme disease

72
Q

under bsl 3

A

high containment
aerosol transmission
serious

ex: tuberculosis

73
Q

under bsl 4

A

max containment
life threatening

ex: e-bola

74
Q

the color code of health

A

blue

75
Q

color code of flammability

A

red

76
Q

color code of reactivity

A

yellow

MICROLAB (1 decks)

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