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MICROLAB > MODULE 0 AND 1 > Flashcards

MODULE 0 AND 1 Flashcards

1
Q

lens, usually 10x in magnification, where you look through in the microscope

A

ocular/eyepiece

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2
Q

revolving nosepiece

A

where the objectives are attached

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3
Q

arm

A

where the microscope is grasped

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4
Q

objective lenses

A

lenses located above the stage

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5
Q

stage

A

platform under objectves where the specimen is placed

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6
Q

stage adjustment knobs

A

moves the slide left or right and forward or backward

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7
Q

lamp

A

built in light source for electric microscope

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8
Q

aperture diaphragm control

A

regulates the amount of light reaching the specimen

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9
Q

condenser

A

lens located below the stage which collects light and delivers it to the objective

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10
Q

coarse focus adjustment

A

focuses the specimen under the scanner and LPO

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11
Q

fine focus adjustment

A

focuses the specimen under hpo and oio

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12
Q

three basic parts of a microscope

A

mechanical, magnifying, illuminating

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13
Q

these are under illuminatio

A

lamp, condenser, iris diaphragm

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14
Q

under magnification

A

objectives and eyepiece

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15
Q

under mechanical

A

stage, coarse and fine knobs, revolving nosepiece, and stage adjustment knobs and slide holder

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16
Q

the shape of bacillus subtilis

A

rod-shaped

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17
Q

domain of neisseria subflava

A

bacteria

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18
Q

ability of a microscope lens to gather fine details of the specimen being discovered

A

limit or resolution / resolving power

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19
Q

formula of limit of resolution

A

abbe’s equation

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20
Q

states that resolution is defined by the wavelength of light and the NA

A

abbe’s equation

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21
Q

the constant value derived from the properties of light

A

0.61

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22
Q

wavelength of visible light

A

lambda

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23
Q

the refractive index of the medium between objective lens and glass slide

A

eta

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24
Q

half the value of the angular aperture

A

theta

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25
cone angle; cone of light that enters the objective lens
angular aperture
26
baker's yeast
fungi; photosynthetic absent
27
ciliate
protozoa; absent
28
chlamydomonas reinhardtii
algae; present
29
e.coli
bacteria; absent
30
paramecium caudatum
protozoa; absent
31
euglena
protozoa; present
32
meat mold
fungi; absent
33
spirogyra
algae; present
34
eukaryotic microbes that comprises the unicellular fungi
yeasts
35
eukaryotic cells are only multicellular
false
36
molds
filamentous fungi
37
filamentous cells of molds
hyphae
38
masses of hyphae
mycelia
39
motility structure for amoebae
pseudopodia
40
these composed the numerical aperture of a microscope
refractive index, angular aperture, working distance
41
the eta value of air
1.00
42
eta value of water
1.33
43
eta value of oil
1.5
44
the ability to achieve a clear image, typically achieved by moving either the eyepiece tubes or the stage
focus
45
determined by multiplying the magnification capability of the eyepiece lens by that of the objective lens.
total magnification
46
The lens closest to the specimen that first receives the rays from the specimen (the object) and forms the image in the focal plane of the eyepiece is the OCULAR.
false; should be objective lens
47
The platform on which slides and specimens are placed for viewing refers to the STAGE CLIPS.
stage
48
The COURSE ADJUSTMENT refers to the knob on the side of the microscope that moves the objective lens up and down
true
49
heterogeneous group of photosynthetic organisms
algae
50
these are composed of cyanobacteria
prokaryotic algae
51
diatoms, dinoflagellates, many green algae are examples of
eukaryotic algae
52
where the specimen being viewed dark against a light background
bright-field microscope
53
to keep bad bugs away from people
biosafety
54
to keep bad people away from bugs
biosecurity
55
the most effective in the hierarchy of controls
elimination
56
this is the order of hierarchy (from least to most effective
ppe > administrative controls > engineering controls > substitution > elimination
57
this means removing the hazard
eliminatiion
58
replacing the hazard
substitution
59
isolating people from the hazard
engineering controls
60
changing the way people work
administrative controls
61
protecting the worker with this
ppe
62
key considerations in ppe selection
consider potential routes of infection and exposure consider what the ppe is protecting consider the limitations for the use of types of ppe consider how and where ppe should be donned and doffed
63
these are the personal protection requirements and practices in a microbiology lab (bench top work
1. lab gowns 2. closed toe shoes 3. safety goggles 4. gloves 5. handwashing
64
this is the sequence for putting on ppe
gown, mask, goggles, gloves
65
how to handle laboratory spills
``` stop what you are doing presume you are contaminated inform others in the area of spill localize the spilled materials label the area as contaminated ```
66
the correct waste hierarchy from least to highest
biological > chemical > radioactive
67
also known as biomedical waste/infectious waste
biological waste
68
the color coding of waste
``` black (general waste) green (wet waste) yellow (infectious waste) yellow with black band (chemical and pharmaceutical waste) orange (radioactive waste) sharps collections ```
69
these are routes of exposure
contact, inhalation, ingestion, percutaneous
70
These are under BSL 1
no containment defined organisms unlikely to cause disease ex: e-coli
71
under bsl 2
containment moderate risks varying severity ex: influenza, hiv, lyme disease
72
under bsl 3
high containment aerosol transmission serious ex: tuberculosis
73
under bsl 4
max containment life threatening ex: e-bola
74
the color code of health
blue
75
color code of flammability
red
76
color code of reactivity
yellow

MICROLAB (1 decks)

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