modern genetics (7) Flashcards
1
Q
what is the use of PCR
A
to amplify DNA samples that contain specific sequences
2
Q
why is PCR done before other techniques
A
- the DNA needs to be amplified
- so that there is enough for it to be visible after staining or enough for a sequencing machine to work
3
Q
outline the process of PCR
A
use high temps (95C) -> to separate strands
lower temps (55C) -> to bind primers
heat again (72C) -> to replicate DNA as its DNA polymerases optimum temp
repeat processes 20-30 times -> to obtain enough copies of the DNA
4
Q
why is everything put in a buffer solution
A
to maintain a steady pH
5
Q
what are primers
A
short DNA strands that are complementary to the sample DNA
6
Q
what is DNA profiling
A
when they identify individuals (and relatives) using DNA samples
it normally uses differences in non-coding DNA -> meaning we cannot tell the phenotype
