modern chemotherapy

Question 1

what is a polyclonal antibody

Answer 1

mixture of antibodies produced by different clones of plasma B cells - cheap but lots of s/e

Question 2

what are monoclonal antibodies

Answer 2

antibodies produced by one clone of plasma B cells - reproducible but prone to mutations

Question 3

what are the different parts of an ADC

Answer 3

antibody - cell selectivity and half life
linker-antibody attachment - bioconjugation (cleavable or non-cleavable)
linker-drug attachment - cleavable/non
payload - cytotoxic drug

Question 4

what are two examples of ADCs

Answer 4

trastuzumab - non-cleavable linker
brentixumab - attached to cysteine in hingre region on MAB

Question 5

what are the 3 types of linkers that can be used in DACs

Answer 5

hydrazone - serum stability and degradation intracellularly
peptide - enzymatically hydrolysed by lysosomal peptides
thioether - stable and non-reducible - only released when MAB degraded

Question 6

which 4 points need to be considered when designing a ADC

Answer 6

o Amino acid used to conjugate thinker – lysine or cysteine
o Functional group used to attach to the chosen amino acid
o Functional group used to attach to the cytotoxic drug
o Whether the linker is cleavable or not

Question 7

what are the two challenges associated with ADCs

Answer 7

solubility - hydrophobic drugs will aggregate and will not be soluble
drug loading - variability