MLS Flashcards
analytes
substance or constituent in which the laboratory conducts testing?
chelation of calcium in the EDTA tube?
Prevents coagulation by inhibiting coagulation
common application of the polarized light microscope in the clinical laboratory do?
Study crystals in urine and in cells
The rate at which a glucose molecule attaches to hemoglobin to form a ketoamine is **directly/indirectly **proportional to the glucose concentration in the plasma.
Directly proportional
What characteristics/functions do calibrators have?
Contains a known amount of analyte being tested
Calibrators are used to establish a correlation between the measurement response and the concentration or amount of the substance that is being measured by the test procedure.
Contains a known amount of analyte being tested
Chemiluminescence
immunoassay methodology is **the emission of light by molecules **in an excited state with a limited amount of heat as the result of a chemical reaction?
Biosafety level of 2+ or 3
serious or potentially lethal dz with respiratory transmission
Biosafety level laboratory operate to handle TB sputum and TM materials?
Absorbance can be calculated easily from percent transmission using which calculation?
2 – log10 %T
What refers to the reproducibility of test results?
Precision
Class B fire extinguishers used for?
.
Flammable liquid
formula is used to convert Celsius to Kelvin?
.
Celsius plus 273
organization requires training in chemical hazards and infectious materials for new testing personnel?
OSHA
nearest degree, what is 40° Fahrenheit converted to Celsius?
4° C
Why do laboratories record temperature and humidity levels daily?
Out-of-control conditions could be caused by changes in temperature and humidity
the name of the substance in which solute is dissolved in?
Solvent
Which type of microscope objective should never be used with oil?
40x objective
How many milliliters (mL) of 0.25 M NaOH are needed to make 100 mL of a 0.05 M solution of NaOH?
20 mL
What process helps monitor the accuracy and reproducibility of results using controlled specimens?
Quality control
In the calculation of MCV = HCT x 10/___?
RBC
Category B,
Bioterorism agents category: moderate
(suffering from the disease) with low mortality (death) rates.
How do you convert Celsius to Fahrenheit?
F=(Celsius degrees number x 9/5) + 32
Lipemic
milky-white plasma or serum may interfere with chemical determinations, such as triglyceride or nephelometric assays?
A microscope should never be left with the __________ in place.
oil-immersion objective
Which three veins are most frequently used for a venipuncture?
Cephalic, basilic, and median cubita
Which substance is employed to produce a chemical reaction?
Reagent
How many grams of sulfosalicylic acid (MW = 254) are required to prepare 1 L of a 3% (w/v) solution?
30
3 * 1000/100 = 3*10 = 30 grams
What can result from failing to apply sufficient pressure to a venipuncture site?
Hematoma
disorders requires the observation of oligoclonal immunoglobulin bands on cerebrospinal fluid (CSF) protein electrophoresis?
Multiple sclerosis
Which analyte rises first in response to inflammation?
C-reactive protein
Which of the following is the most widely used tumor marker for colorectal cancer?
Carcinoembryonic antigen
determinations is used to assess the total body lead poisoning burden?
Whole blood lead
Which of the following associations of creatine kinase and disease condition is correct?
CK-MB and cardiac disorders
Which of the following levels do parathyroid glands either increase or decrease PTH secretion in response to changes?
Calcium
What does a biuret reaction for total protein analysis depend on?
Peptide bonds
Which of the following is primarily evaluated in an ALT assessment?
Hepatic disorders
Which of the following diseases or conditions results from an incompatibility between maternal and fetal blood?
Hemolytic disease of the newborn
How is the formation of HbA1c related to plasma glucose concentration?
Directly proportional
Which of the following is prolonged in tourniquet use cause?
Hyperkalemia
What is the function of the pituitary gland?
Stimulates the other glands to produce hormones as needed
In which organ are most plasma proteins synthesized?
Liver
Which tumor marker is an abundant serum protein and is normally synthesized by the fetal liver?
Alpha-fetoprotein
Which condition would most likely be the cause of an increased ALP level in an adult?
Liver disease
What is secreted by the alpha cells of the pancreas and is the major hormone that opposes the action of insulin, therefore increasing blood glucose by stimulating the breakdown of glycogen in the liver
Glucagon
Which of the following is one of the most common clinical applications of GC-MS analysis?
Drug testing
Which two hormones are produced by the thyroid gland from the trace mineral iodine and are responsible for the regulation of metabolic activities in all body cells?
Thyroxine and triiodothyronine
Which class does the enzyme, aspartate aminotransferase (AST), belong to?
Transferases
What are the two main groups of plasma proteins?
Albumin and globulin
What is a glycoprotein hormone that is secreted by the kidney and functions to increase the production of red blood cells?
Erythropoietin
Which is a condition involving the deposition of plaques in the blood vessels that has been proven to lead to coronary artery disease?
Atherosclerosis
What are the two most common aminotransferases measured in the clinical laboratory?
AST and ALT
The measurement of the decrease in intensity of an incident light beam as it passes through a solution of particles defines which of the following methods?
Turbidimetry
In what manner is the dye uptake by a sample following electrophoresis and staining related to the sample concentration?
Directly proportional
Which of the following are the two methods used to characterize monoclonal proteins?
- Immunoelectrophoresis
- immunofixation electrophoresis
Which of the following indicates a borderline triglyceride range?
150 – 199 mg/dL
Which tissue is richest in acid phosphatase?
Prostate
The nephrons of the kidneys have five basic parts. Which part is also referred to as the Bowman’s capsule?
Glomerulus
Which of the following is a non-insulin dependent diabetes, is characterized by insulin resistance and progressive hyperglycemia, and is called “adult-onset diabetes”?
Type 2
What is an inflammation-sensitive protein that can be measured by immunoassays?
C-reactive protein (CRP)
Brain isoenzyme
Creatinine kinase can be separated into three distinct molecular forms. CK-BB is mainly indicated by which tissue?
Which of the following gives serum its characteristic milky appearance (lipemia) when blood is drawn after a meal?
Chylomicrons
What is the term for the mathematical modeling of drug concentration in circulation, which helps assist in establishing a dosage regimen?
Pharmacokinetics
What tumor marker is most useful in the diagnosis of ovarian and endometrial carcinomas?
CA 125
Elevation of which enzymes are found in hepatocellular disorders?
AST, ALT
What is the yellow discoloration of the plasma, skin, and mucous membranes that is caused by the abnormal metabolism, accumulation, or retention of bilirubin called?
Jaundice
What is derived from the iron-containing heme portion of hemoglobin, which is released from the breakdown of RBCs?
Bilirubin
Which is the key membrane component for a potassium selective electrode?
Valinomycin
Which term refers to the mathematical difference between the anions (CL- and HCO3-) and the cations (Na+ and K+)?
Anion gap
Which enzyme may be elevated for up to 48 hours after mild to strenuous activity or exercise?
CK
What does a manual reticulocyte count do?
d.
Uses a vital stain which can be a biological hazard
What is indicated by a large red cell distribution curve on an automated blood count?
Abnormal red cell size variation
What causes patients on radiation and chemotherapy to develop bleeding problems?
Decrease in thrombocytes
Which leukocyte responds to bacterial infections?
Neutrophil
What hematological disease/disorder has an increased number of immature cells?
Acute leukemia
Formalin
is used as a fixative to fix bone marrow particles from core biopsy specimen?
What is a Hematology stain that is used to make white blood cell differential smears?
Wright stain
Which of the following is an immature granulocyte with a non-segmented nucleus?
Band neutrophil
Which cellular constituent of blood contains the vital protein hemoglobin?
Erythrocytes
Which term refers to an abnormal distribution pattern of red blood cells, in which cells stick together or are aligned in aggregates resembling stacks of coins?
Rouleaux
In most infections, there is some increase in the ESR. What are two exceptions?
Chorea and undulant fever
Who developed the rule that is used in quality control to evaluate results when two or more levels of control material are used?
Westgard
As the neutrophil matures, which neutrophil has reddish-purple primary granules that help to identify the cell?
Promyelocyte
When performing a bone marrow differential count, which cell is NOT included?
Megakaryocytes
What type of cell does the monocyte transition into once it leaves the blood and enters the tissue?
Macrophage
What cells differentiate further in the bone marrow while encountering an antigen?
B lymphocytes
What is used to evaluate size distribution curve of leukocyte data and allows visualization of subpopulation of cells based on their relative sizes?
Histogram
graph that display continous numeric values
What color top can the additive EDTA be found in?
Lavender top
Which macrocytic anemias are the results of vitamin B12 or folic acid deficiency, or a combination of both?
Megaloblastic anemias
What is the name of nucleated red blood cells or reticulocytes in the peripheral blood?
Shift cells
Shift cells are nucleated red blood cells or polychromatic macrocytes
Which erythrocyte index indicates whether the red blood cells will appear microcytic, normocytic, or macrocytic?
MCV
What are erythrocytes referred to that are larger in size?
Macrocytes
What is the rate at which red blood cells settle to the bottom of a test tube?
Erythrocyte sedimentation rate
Which automated blood cell counting principle is based on increased resistance that occurs when a blood cell with poor conductivity passes through an electrical field?
Impedance
When performing the Westergren method, fresh anticoagulated blood collected in sodium citrate is the preferred anticoagulant. What is the ratio of blood to sodium citrate?
4 vol of blood to 1 vol of sodium citrate
4:1
What is the process in which a cell engulfs and digests foreign material?
Phagocytosis
What is a white blood cell known as in human blood?
Leukocyte
What is the hematology orderable test that consists of analytes such as hemoglobin, hematocrit, red blood cell count with morphology, white blood cell count with differential, and platelet estimate?
counting everything
Complete blood count
Morphologically, what are anemias generally classified as?
Normochromic-normocytic,
macrocytic,
hypochromic-microcytic
Which term is used to describe a red blood cell with a normal shape?
Discocyte
When evaluating an* erythrocyte histogram, a shift to the left* of the erythrocyte series should correspond to which type of erythrocytes?
Microcytes