Mixtures

Question 1

What is allelic dropout?

Answer 1

• Allele dropout occurs when a sample is tight and one or more alleles are not present.
• This can be due to a number of factors:
  
  • Initial input of DNA was too low, resulting in a failure to amplify one or more alleles in the sample
  • A mutation in the primer binding site is present, which causes failure in amplification of the allele.
  • An allele sizes outside of the normal range for a particular locus and goes undetected.