Miscellaneous Gram neg bacilli

Question 1

Glucose-cystine blood agar is the media of choice for which bacteria

Answer 1

Francisella

Question 2

• 10% CO2 in humidified air
• 3-4 weeeks for growth
• Strict aerobe
• Oxidase : +
• Catalase : +
• Considered potential bioterrorism agent ( Biosafety level3 )
• Isolated from
A. Blood
B. BM

Answer 2

Brucella

Question 3

In which media Brucella grow

Answer 3

Brucella buffered charcoal yeast extract (BCYE)

Question 4

Colonies : appear like mercury droplets

Answer 4

Bordetella

Question 5

A. Cellulitis
B. Gum disease
• Will not grow on MAC
• Catalase +
• Glucose: +

Answer 5

Actinobacillus

Question 6

• Cause cellulitis
• Grow on non selective agars but not MAC
• Oxidase pos
• Catalase pos
• Indole pos
• Nitrate pos
• Nonmotile
• Gram neg coccobacilli (bipolar staining)
• Very susceptible to penicillin

Answer 6

Pasteurella

Question 7

This is a selective media for which organism :
A. Bordet gengou ( potato infusion )
B. Regan Lowe agar

Answer 7

Bordetella

Question 8

• 50% of the strains corrode / pit the agar surface

Answer 8

Eikenella corrodens

Question 9

• Require hemain (factor X) for growth unless 5-10% CO2 is present
• Produce bleach like odor
• Gram neg bacilli

Answer 9

Eikenella corrodens :

Question 10

• Require L-cystine for growth
• Will grow on BCYE , but will not grow on SBA
• And it will grow on Brucella blood agar
• Asacchrolytic
• Most biochemical test are neg
• Autofluoresce
• Resistant to commonly used concentration of chlorine

Answer 10

Legionella

Question 11

Cause :

A. Bacillary angiomatosis

B. Trench fever

Answer 11

B.quintana

Question 12

B.henselae : cause

Answer 12

cat-scratch disease