Miscellaneous

Question 1

What is the purpose of ELISA?

Answer 1

enzyme-linked immunosorbent assay

this identifies the concentration of a molecule of interest (often antibodies and antigens)

Question 2

What is the purpose of gel electrophoresis?

Answer 2

to separate proteins, DNA or RNA based on size and/or charge

Question 3

What are the 4 types of gel electrophoresis?

Answer 3

1. native PAGE
2. SDS PAGE
3. Reducing SDS PAGE
4. Isoelectric Focusing

Question 4

What is separated in native PAGE?

Answer 4

“Native” means NO denaturing agents are used

proteins are separated by size AND charge

Question 5

What is separated in SDS Page?

Answer 5

proteins separated by ONLY mass

Will only interrupt
non-covalent bonds

B/c Disulfide bridges are still intact, this is useful to analyze proteins with multiple subunits

Question 6

What is separated in Reducing SDS PAGE?

Answer 6

proteins separated by ONLY molecular weight

Disulfide bridges are reduced, resulting in a completely denatured protein

Question 7

What is separated in isoelectric focusing?

Answer 7

proteins separated by ONLY charge