Misc. Hematological Testing Flashcards
What is the principle of Microhematocrit defined as?
Percentage of total blood volume occupied by Erythrocytes
Packet RBC Volume
What involves the use of clarifying anemia, evaluating fluid status, and monitoring hemorrhagic conditions?
Microhematocrit
What is the QC associated with Microhematocrit?
Ran in duplicate
Must agree within +- 1
What are the references ranges for Hemtocrit?
Male: 42%-52%
Female: 37%-47%
Newborn: 53%-65%
What is a nonspecific screening test for inflammation?
Erythrocyte Sedimentation Rate (ESR)
Distance at which RBCs settle per hour (mm/h)
Where do we see elevations of Erythrocyte Sedimentation Rate (ESR)?
Myocardial Infarction
Bacterial Infection
What needs to be used for a specimen for ESR?
Whole blood EDTA
Not hemolyzed, free of clots and fibrin
Must be brought to room temperature
What is the procedure for running an ESR test?
Tube must stand vertically to prevent the alteration of rate of sedimentation
60 minute timer
What are the reference ranges for ESR?
<50 >50
Male: 0-15 mm/hr 0-20mm/hr
Female: 0-20 mm/hr 0-30mm/hr
What are the factors that can affect the rate of settlement?
Westergren tube not filled correctly
Vibration and angles
Cold Agglutinins
Rouleaux, Globulin, Fibrinogen increase
Clots and Fibrin
What must we do to Reticulocytes before they can be quantified?
Stain the with Supravital Stain (New Methylene Blue)
Room Temp for 8 hours
What is the formula to calculate Reticulocyte percentage?
%Reticulocyte = #Reticulocytes*100/1000
What are the Retic ranges for infants and adults?
Adults: 0.5% - 2.0%
Infants: 2.0% - 6.0%
What are medications such as Levodopa, Malarial medications, Corticotropin, and fever reducing medications responsible for?
An increase in the interpretation of Reticulocytes
What are we testing for when we do a Sickle Cell Screening?
Hemoglobin S