Minor Crossmatching

Question 1

what is the principle of minor crossmatching

Answer 1

this detects the presence of antibodies in the donor’s serum that will damage or destroy the patient’s RBCs

Question 2

materials for minor crossmatching

Answer 2

• 5 ml test tube
• NSS
• Water bath
• AHG reagent
• Centrifuge
• Microscope

Question 3

specimen used for minor crossmatching

Answer 3

a clotted or anticoagulated blood drawn within 72 hours of testing may be used

Question 4

**fresh serum ** is used to assure the presence of?

Answer 4

adequate complement and calcium

Question 5

immediate spin/saline phase

Answer 5

1. Place 2 drops of donor’s serum in a 5 ml test tube.
2. Add 1 drop of 3-5% patient’s red cells.
3. Mix and centrifuge at 3400 rpm for 15 seconds then read
   for the presence of agglutination or hemolysis.

Question 6

protein phase/thermal phase

Answer 6

4. Add 2 drops of 22% bovine albumin.
5. Mix and incubate at 37 deg C for 15-30 minutes.
6. Centrifuge for 15 seconds at 3400 rpm then observe for
   agglutination or hemolysis.

Question 7

AHG phase

Answer 7

7. Wash with NSS three to four times.
8. Add two drops of AHG reagent.
9. Mix and centrifuge for 15 seconds at 3400 rpm.
10. Read for hemolysis or agglutination with or without microscope.
11. Add 1 drop of check cells if negative.