Milk Grading and Processing Flashcards
top 3 milk producers
- India
- EU
- US
functional unit for milk production
alveolus of mammary gland (udder)
two layers of cells that make up mammary gland
- lactocytes (mammary epithelial cells)
- basal myoepithelial cells
what do lactocytes do, where are they located
- milk producing cells
- located on surface inside mammary gland
what do basal myoepithelium cells do, where are they located
- like smooth muscle
- located surrounding lactocytes
what is each alveolus surrounded by
- capillaries
- to get nutrients
- synthesize and express milk
how much blood is needed to form 1 L of milk
~800-900 L of blood
how is milk secretion from mammary gland performed
myoepithelial cells squeeze each alveolus to discharge milk into lumen of ductules under influence of oxytocin
which hormone is required to release milk from udder
oxytocin (released from pituitary gland)
where is milk stored
60-80% in alveoli
20-40% in cistern
true or false: each quarter of a cow udder is independently working
true
- gland cistern
- teat cistern
- teat canal
- alveolus
how long and when is colostrum production, how long is milk production
- colostrum production starts nearly pre partum and continues post partum
- colostrum produced for 3-5 days
- milk production for ~305 days
comparison of colostrum and mil appearance
- colostrum
- viscous, thick, waxy, yellowish
- milk
- thin, liquified, white
comparison of colostrum to milk - dry matter, minerals, proteins, fat soluble vitamins
- colostrum has 2x more dry matter
- colostrum has 3x more minerals
- colostrum has 5x more proteins
- colostrum has more fat soluble vitamins
is colostrum suitable for processing, should it be shipped to milk processing facilities
colostrum is not suitable for processing and should not be shipped to milk processing facilities
true or false: milk is nearly a complete diet and does not have pH and moisture danger zones
false - milk is nearly a complete diet but does have pH and moisture danger zone naturally
milk composition
- 87% water
- 4.9% carbs
- 3.4% fat
- 3.3% protein
- 1.4% vitamins and minerals
what does the deviation of milk compositions from normal proportions indicate
adulteration or contamination of milk
milk fat composition
65% saturated fat
29% monounsaturated fat
6% polyunsaturated fat
what is a saturated fat
type of fat in which the fatty acid chains have all single bonds
two broad categories of milk proteins based on solubility in water
- whey proteins (20%) - albumin, antibodies
- caseins (80%)
which milk proteins are soluble, which are insoluble
- whey proteins are soluble
- caseins are insoluble
whey protein fractions
- B-lactoglobulin - 49%
- a-lactalbumin - 18%
- protease peptone fraction - 15%
- immunoglobulins - 12%
- blood serum albumin - 6%
casein proteins fractions
- caseins as1-casein - 40%
- caseins B-casein - 34%
- caseins k-casein - 12%
- caseins as2-casein - 10%
- caseins y-casein - 4%
which milk protein is the major raw material for cheese making
caseins
vitamins and minerals found in large amounts in milk
- vitamin B12 (cobalamin)
- vitamin B2 (riboflavin)
- calcium
- phosphorous
testing milk aroma
if off-odor retest
ensure sample not too hot
if off odor still persists get second opinion from plant manager
testing milk pH
normal - 6.5-6.8 (at 25C)
ensure milk sample at correct temperature and pH meter is calibrated
if pH is out of range contact plant manager and retest
if retest <6.5 or >6.8, reject
testing milk titratable acidity
normal - 0.13-0.17
if retest between 0.13 and 0.18 milk may be ok if all other tests ok
discuss history of load
if retest <0.13 or >0.17, reject
seven major parameters routinely checked by regulatory agencies for quality raw milk production
- somatic cell count - 750,000 cells/mL
- bacteria count - 100,000 cfu/mL
- organoleptic quality - color, odor, taste, consistency
- milk chemical composition (nutrition) - water, fat, protein, minerals
- adulteration or residues
- pH - 6.5-6.8
- temperature - <4C
what nutritional composition must regulated milk have
- 87% water
- > 3.325% fat
- > 8.25% non fat solids
what are somatic cell counts related to
mastits
what SCC must regulated raw milk have
less than 750,000 SCC/mL
how are bacterial counts in milk measured
standard plate count (aerobic colony count)
bacteria counts of regulated milk
less than 100,000 CFU/mL
bacterial count by standard plate count
- targets general bacteria
- counts overall number of aerobic bacteria in milk
bacteria count by preliminary incubation count
- targets sub groups - cold loving bacteria
- involves holding milk at 12.8C for 18 hours prior to plating
- detects bacteria that grow well at fridge temperatures (psychrotrophic bacteria)
bacterial counts by lab pasteurization count
- targets sub groups - heat resistant bacteria
- involves holding milk at 63.8C for 30 minutes prior to plating
- bacteria that survive heat treatment (thermoduric bacteria) are enumerated using standard plate count
bacterial counts by coliform bacteria counts
- targets sub groups - fecal or environmental bacteria
- selects for bacteria most associated with manure or environmental contamination
- requires selective media for coliforms and inhibiting other bacteria
most frequent cause of high SPC bacterial counts
poor cleaning of milking systems
sources of high bacterial counts
- mastitis - staph aureus, streptococcus, coliforms
- soiled cows
- unsanitary milking conditions
- failure to cool milk rapidly to less than 4C
- failure of water heater
normal color of milk
whitish
normal odor of milk
no off-odor
normal taste of milk
pleasantly sweet
normal consistency of milk
liquid but not watery
three major sources or causes of bad flavor and odor development in milk
- absorbed or transmitted form environment
- bacterial or microbial
- chemical, enzymatic, processing
how does absorbed or transmitted from environment affect milk flavor
feedy, barny, cowy, weedy, unclean, lacks freshness, stale, refrigerator or cooler odors
how does bacteria or microbes affect milk flavor
acid, bitter, malty, lacks freshness, unclean, fruity or fermented, putrid, rancid
how does chemical, enzymatic, processing affect milk flavor
cowy (ketosis), salty, rancid, bitter, oxidized, sunlight, foreign, astringent, medicinal, flat, cooked
organoleptic characteristic of typical normal milk flavor
very little distinct odor
pleasantly sweet
clean with no aftertaste
organoleptic characteristic of acid (sour) milk
sour like lemon due to lactic acid production by microbes due to poor refrigeration
organoleptic characteristic of barny milk
barn (feces) odor due to ventilation and unclean barn conditions or ketosis
organoleptic characteristic of bitter (beer taste) milk
bitter like hops in beer or coffee due to breakdown of milk proteins by microbial enzymes
organoleptic characteristic of carton/paperboard milk
resembles freshly dampened cardboard becasue milk absorbed odor of carton pack
organoleptic characteristic of cooked milk flavor
suggestive of boiled milk
results from improper pasteurization
organoleptic characteristic of cowy flavor milk
cow breath or fecal odor due to acetone such as during ketosis or acetonemia
organoleptic characteristic of feed flavor milk
milk has silage, hay, grassy flavor
feeding should be done after milking as milk absorbs odor
poor ventilation could also cause feed flavor
organoleptic characteristic of flat (watery) milk flavor
loss of normal flavor of milk due to adulteration, particularly water addition
organoleptic characteristic of garlic or oniony milk flavor
cows may consume garlic weed
organoleptic characteristic of rancid (soapy) milk flavor
putrified fat due to release of free fatty acid by microbial destruction of milk fat
organoleptic characteristic of salty milk flavor
like salt, which is linked to late lactation or mastitis
advantages to grading milk
- standardizes milk prices
- determines type of milk sold in liquid form
- determines type of milk that requires further processing (cheese) before consumption
criteria for milk grading
- chemical constituents (water, fat, non fat)
- load of bacteria count
- bulk tank SCC
- organoleptic features of milk
milk grades based on bacteria counts
- certified milk - <10,000 cfu/mL - expensive milk (for famous people)
- grade A - < 100,000 cfu/mL - supermarket milk
- grade B - <500,000 cfu/mL - low quality
what is grade B milk used for
cheese
further processed products
illegal to sell as milk
characteristics of certified milk
- 3.5-4% fat
- 8-8.5% total solid non fat
- <10,000 cfu/mL aerobic bacteria count
- 0 zero tolerance coliform plate count
- <0.17% total acidity
characteristics of grade A milk
- <750,000 SCC
- <100,000 aerobic bacterial count
- <10 cfu/mL zero tolerance coliform plate count
- <0.02% total acidity
characteristics of grade B milk
- <1,000,000 SCC
- < 500,000 aerobic bacteria count
- <100 zero tolerance coliform plate count
- > 0.2% total acidity
- <0.75 mg/O.5 L milk foreign particles
pasteruization temperature
> 63 C
kill microbes
4 kinds of milk pasteruization
- ultra high temp
- 125-140 C for 2-4 sec
- long shelf life (>6 months)
- canned sterilization
- 115-120 C for 15-20 min
- high temp short time
- 72-74 C for 15-20 sec
- widely used
- low temp long time (batch)
- 63 C for 30 minutes
yogurt processing
- 40-45 C for 15 min
cheese processing
- 30-35 C for 15 min
thermization
- 57-68 C for 15 min
- targets pathogenic bacteria
- leaves good bacteria
- does not alter structure and taste of milk
batch pasteruization
- low temp high time
- 63 C for 30 min
- alteration in milk protein, structure, and taste
flash pasteurization
- high temp short time
- 72-74 C for 15-20 sec
ultra high temperature pasteurization
- 135-140 C for 2-4 sec
- targets coxiella burnetii (Q fever)
- kills vegetative forms of bacteria and milk can survive for 9 months
canned sterilization
- wet treatment of canned milk products in autoclave/specialized treatment chamber
- 115-121 C for 10-20 min
basic steps in milk processing
separating its components
- raw milk
- water 87.4%
- milk solids 12.6%
- milk fat 3.6%
- milk solids non fat 9%
- minerals 0.7%
- lactose 4.9%
- protein 3.4%
- casein 2.7%
- whey protein 0.7%
nano pore membrane technology
reverse osmosis (RO), nanofiltration (NF), ultrafiltration (UF), microfiltration (MF)
milk processing - collection
- collected rom dairies
- stored in storage tanks
- cooled to certain temp
- transferred to refrigerated tanks
milk processing - separation
- sediment, debris, bacteria removed from raw milk
- heavier milk fat and lighter milk separated to get skim milk and cream
milk processing - pasteurization
- milk heated to specific temp for specific time
- cooling again without allowing recontamination
milk processing - standardizing
- reintroducing 1%, 2%, 3.5% fat
milk processing - homogenizing
- raw milk pushed through atomizer to form tiny particles to disperse fat evenly
- stops fat from floating to top of container
- done by breaking down the fat particles
milk processing - packaging
- cartons for retail or used further for manufacturing other dairy products
cheese processing
- standardize milk (protein to fat ratio)
- pasteurize 63C or heat treat 30-35C
- cool to 32C for starter bacteria
- inoculate with starter and non starter bacteria, hold at 32C for 30 min to ripen
- add rennet and form curd and curds not disturbed for 30 min
- ferment until pH 6.4, cut curd, heat at 38C to separate curds from whey
- drain whey
- cut into blocks, piling and flipping until pH 5.1-5.5 and cut into pieces
- sprinkle dry alt or brine (salt water solution) onto vats
- form into blocks by placing cheese in hoops (molds)
- store in coolers for aging (months to years)
- package
starter bacteria for cheese making
- lactococcus lactis
- streptococcus thermophilus
- lactobacillus sp
what is rennet
enzyme cleaving casein
top 5 biological hazards associated with milk
listeria monocytogenes
e coli
salmonella
infested with moulds or yeasts
staphylococcus
milk quality assessmnet
20 GHP
12 HACCP
5 animal welfare (including avoiding milking cows with 5Ds)
7 milk quality assessment methods
7 milk quality assessment methods
SCC
bacteria count
milk composition
organoleptic
pH
temperature
5Ds of milking cows
- distorted milk
- drug residues in milk
- devastated animal
- diseased animal
- discharges from animal