MIDTERMS TOPIC 2

Question 1

classification based on temperature requirement

Answer 1

academic or bergey’s classification

Question 2

what are the 4 groups in bergey’s or academic classification?

Answer 2

• pyogenes grp
• viridans grp
• lactic grp
• enterococcus grp

Question 3

45 (x)
37(/)
10 (x)

Answer 3

pyogenic grp

Question 4

45 (/)
37(/)
10 (x)

Answer 4

viridans grp

Question 5

45 (x)
37(/)
10 (/)

Answer 5

lactic grp

Question 6

45 (/)
37(/)
10 (/)

Answer 6

enterococcus grp

Question 7

what grp produce pus; mostly β-hemolytic

Answer 7

pyogenes grp

Question 8

• not part of the Lancefield group: resist Lancefield
  Precipitation Test
• α-hemolytic or non-hemolytic
• normal biota in URT in humans

Answer 8

viridans grp

Question 9

• non-hemoly=c with Lancefield N an=gen
• found in dairy products
• give one spp. that belongs to this grp

Answer 9

• lactic grp
• S. lactic

Question 10

• normal of human intestine
• give one spp. that belongs to this grp

Answer 10

• enterococcus grp
• e. faecalis

Question 11

classification based on the hemolytic pattern on BAP

Answer 11

smith and brown’s classification

Question 12

• partial/incomplete hemolysis of RBC around colonies
• Culture: greenish or brownish discoloration around colony

Answer 12

alpha-hemolytic streptococci

Question 13

• complete hemolysis of RBC around colony
• culture: clear zone around colony

Answer 13

beta-hemolytic streptococci

Question 14

• exhibit NO hemolysis of RBC around colony
• Culture: RBC surrounding colony are not affected (no
  change)

Answer 14

gamma-hemolytic streptococci

Question 15

small area of intact RBCs
around colony surrounded by a wider zone of complete
hemolysis

Answer 15

a-prime or wide zone

Question 16

• based on an=genic characteris=cs of GROUP-SPECIFIC C
  SUBSTANCE, a cell wall polysaccharide
• extraction of C carbohydrate from the streptococcal cell
  wall by dilute acid and heating the suspension for 10 mins
• mostly significant in classifying and identifying beta-hemolyti streptococci

Answer 16

lancefield classification

Question 17

• not considered as a part of indigenous flora = pathogenic
• acquired through contaminated droplets released through coughing and sneezing
• resistant to drying and can be recovered from swabs
  several hours after the collection
• colonizes throat and skin on humans

Answer 17

S. pyogenes

Question 18

• major virulence factor of S. pyogenes
• resist
  phagocytosis and adherence of the
  bacterial cell to mucosal cells

Answer 18

M protein

Question 19

2 post-streptococcal dses?

Answer 19

• rheumatic fever
• acute glomerulonephritis

Question 20

• aka FIBRONECTIN-BINDING
  PROTEIN
• adhesion molecule that mediates epithelial cell attachment

Answer 20

protein f

Question 21

• adhesion molecule present in the cell wall that is responsible for the
  adherence into respiratory
  epithelial cells

Answer 21

lipoteichoic acid

Question 22

o weakly immunogenic
o prevents opsonized phagocytosis by PMN or macrophages
o mask bacterial antigens

Answer 22

hyaluronic acid capsule

Question 23

o causes lysis of fibrin clots
o binds plasminogen and activates
the production of plasmin
o allows bacteria to move from
clotted area (spread infection)
o Application: treatment of
pulmonary emboli, coronary artery,
and venous thromboses

Answer 23

streptokinase