Midterms-specimen Collection Flashcards
hours required for glucose, lipids, LPP
8-16 hours
What are increased during exercise
Lactate
FA
ammonia
Inc after 48 hrs of fasting
Serum bilirubin
Inc after 72 hrs of fasting
Plasma triglyceride
High protein diet increases what
Urea
This will increase the urinary secretion
Serotonin
Increases the concentration of glucose
Caffeine
Releases catecholamines from adrenal medulla and brain tissue
Caffeine
Posture: prevent hemodilution or hemoconcentration
Seated/supine
Posture: causes extravascular water to transfer to the vascular system
Standing to supine
Posture: causes constriction of blood vessels and reduction of plasma volume
Supine to sitting
Posture: causes shifting of water and electrolytes into tissue causing hemoconcentration
Sitting to supine
How many minute/s is the recommended application of tourniquet
1 min.
Prolonged tourniquet results to
Hemoconcentration
Anaerobiosis
Increase in plasma NEFA
Tobacco smoking (nicotine)
Increase GGT concentration
Increase plasma conc. of urate and trigylceride
Alcohol ingestion
GGT
Gamma glutamyltransferase
Affects adrenal hormone secretion
Stress
This drugs can elevate liver function enzymes
Hepatotoxic drugs
Can decrease plasma medium
Diuretics
Has larger conc of oxygen than CO2
Arterial blood
Larger conc of CO2
Venous blood
Pumped by the heart to lungs
Venous blood
Pumped by the heart to the body cells
Arterial blood
1st crucial step in sample collection
Proper patient identification
Human blood contains approx. ,how many quarts/liters of whole blood
5 quarts
4.73 L
Whole blood(males)
5-6 liters
Whole blood (females)
4-5 liters
Process by which blood is obtained from a patient’s artery
Arterial puncture
Oxygenated blood with bright red color
Arterial blood
Sites for artery
Radial Brachial Femoral Scalp Umbilical
Hardest to control during bleeding period and usually requires special attention
Arterial bleeding
A process by which blood is obtained from a patient’s vein
Venipuncture
Deoxygenated blood with dark red color
Venous blood
Liquid portion of an unclotted blood or anticoagulated blood
Plasma
Plasma contains what
Fibrinogen
Liquid portion of clotted blood
Serum
Most commonly used method in venipuncture
ETS
Evacuated tube system
3 basic methods in venipuncture
Ets
Needle and syringe
Winged infusion set
Most commonly used veins for venipuncture are located in the
Antecubital fossa
1st site
2nd site
3rd site
(Phlebotomy sites)
In order:
Median cubital vein
Cephalic vein
Basilic vein
Best site for venipuncture
Median cubital
Largest and best anchored vein
Median cubital
Reduce the risk of specimen
Order of draw
Order of draw
B lood culture (yellow) C oagulation (light blue) N on additive (red) H eparin (green) E DTA (lavander) S odium fluoride (gray)
EDTA
Ethylene diamine tetraacetic acid
Apply the tourniquet at least how many inches above the site
3-4 inches
Patients with allergies in alcohol
Chlorhexidrine gluconate
Ethanol testing
Benzalkonium chloride
Standard needle length
1 inch or 1.5 inch
Butterfly needle
1/2-3/4 inches
Complications of venipuncture
Immediate local complication
Late local complication
Late general complication
Anti coagulants
K2 edta
Potassium oxalate
Clot activators
Thrombin
Silica
Glass particle
Anti glycolitics
Sodium fluoride
Lithium iodoacetate
Gel separator
Thixotropic gel
Blood is collected from a skin puncture made with a lancet or similar device
Capillary specimen collection
3 components of capillary blood in skin puncture
Venous
Arterial
Tissue fluid
Recommended sites for skin puncture
1st choice: middle finger
2nd choice: ring finger
Preferred site for newborn
Lateral plantar heel surface
3rd and 4th fingers (site)
Palmar surfaces of the fingers
Least preferred site
Earlobe
Order of draw: microcollection tubes
Edta
Other tubes with additives
Non additives
21 and 22 gauge
Blood collection
23 gauge
Used for children
25 gauge
For butterfly needle
16 to 19 gauge
Therapeutic phlebotomy
Preferred site due to vascularity
Earlobe
Most commonly used site in arterilized capillary blood
Lateral plantar heel surface
Best site for blood gas analysis
Indwelling umbilical artery
Centrifugation requirement
1000-3000 relative centrifugal force
Effects of hemolysis
Affects bilirubin levels
Inhibits lipase enzyme
Interferes with the color reactions
Increased level of analytes
Can cause hemolysis and aerosol infection
Rimming the tube
Can cause lysis and slight elevation in LD and K
Excessive centrifugation
May cause incomplete barrier formation in gel tubes
Insufficient centrifugation
Excessive centrifugation
Greater than 3000 RCF
Insufficient centrifugation
Less than 1000
Specimens that require chilling
Ammonia Blood gases Catecholamines Gastrin Lactic acid
Photosensitive analytes
Bilirubin Beta carotene Folate Porphyrins Vitamin A B6
Other biological specimens for testing
CST Synovial fluid Peritoneal fluid Pleural fluid Pericardial fluid
This top can also be used for lactate and alcohol determinations
Gray top
Can cause increase in potassium
Respinning gel tubes
For microcollection tubes, these are the preferred anticoagulants
Lithium
Ammonium salts of heparin
Blue inversions:
3-4 times
Tubes with clot activator inversions:
5 times
Heparin/ lavander inversions
8 times
Non additive inversions
No inversions
System of ensuring accuracy & precision in the lab by including quality control reagents in every series of measurements
Quality control
A quality control system is designed to:
Sensitivity Specificity Accuracy Precision Reliability
Ability of an analytical method to maintain accuracy & precision over an extended period of time
Reliability
Nearness or closeness of the assayed value to the true or target value
Accuracy
Ability of the analytical method to measure only the analyte of interest
Specificity
Ability of an analytical method to give repeated results on the same sample that agree with one another
Precision
Ability of an analytical method to measure the samllest concentration of the analyte of interest
Sensitivity
Ability of the test to detect the proportion of individuals without the disease who test negatively for the disease
Diagnostic specificity
Kinds of quality control
Internal qc
External qc
Combines with calcium to form an insoluble salt
Oxalate
Combines with calcium in a process called chelation
Edta
Forming weakly dissociated calcium components
Fluoride
Combines with calcium in a non-ionized form
Citrate
Acts as anti thrombin and anti thromboplastin
Heparin
Concentration of citrate
3.2-3.8 g/dl
Conc of heparin
0.2 mg/ml of blood
What are the sample rejection
Lipemic sample
Hemolyzed sample
Icteric sample
Conc of fluoride
10 mg/ml
Conc of edta
1-2 mg/ml