MIDTERMS: L2 - STAPHYLOCOCCI Flashcards
T OR F
- Collect at least 60 mL from 3 sites with 10 mL per bottle and 20 mL per site
- 10 mL for aerobic and another 10 mL for anaerobic
TRUE
T OR F
Delivery time is at maximum, 2 hours
TRUE
Best sample for urine is ______
Best sample for urine is midstream clean catch early morning urine
MOTF
SAU
1. Catalase (+), Coagulase (+)
2. Bacitracin susceptible
1 true
2 false, bacitracin resistant
Test used to differentiate staphylococcus from micrococcus
Modified oxidase (SAU = negative - )
Test used to differentiate staph from strep
Catalase test
SAU - positive
T or F
Staphylococcus epidermidis
- Susceptible to NOVOBIOCIN
True
Test to differentiate SAU from Cons
Coagulase
SAU - coagulase +
Resistant to 5 ug NOVOBIOCIN
Staphylococcus saprophyticus
T OR F
6mm zone size - means resistant or no zone of inhibition
TRUE
- True for staphylococcus saprophyticus
CNA
- Disrupts cell membrane of gram (-)
Colistin
CNA
- Blocks DNA replication and membrane of gram negative
Nalidixic
What makes MSA selective and differential?
Selective - presence of high salt concentration (7.5%)
Differential - presence of mannitol and the indicator phenol red
MSA
- SAU color
- S. epidermidis and S. saprophyticus color
SAU - pink (acid change)
S epi and S sap - yellow (no color change)
T or F
Micrococcus luteus and Enterococcus can also produce yellow colonies in MSA
True