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BACTERIOLOGY LAB > MIDTERMS: L2 - STAPHYLOCOCCI > Flashcards

MIDTERMS: L2 - STAPHYLOCOCCI Flashcards

1
Q

T OR F

  • Collect at least 60 mL from 3 sites with 10 mL per bottle and 20 mL per site
  • 10 mL for aerobic and another 10 mL for anaerobic
A

TRUE

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2
Q

T OR F

Delivery time is at maximum, 2 hours

A

TRUE

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3
Q

Best sample for urine is ______

A

Best sample for urine is midstream clean catch early morning urine

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4
Q

MOTF
SAU
1. Catalase (+), Coagulase (+)
2. Bacitracin susceptible

A

1 true

2 false, bacitracin resistant

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5
Q

Test used to differentiate staphylococcus from micrococcus

A 
Modified oxidase
(SAU = negative - )
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6
Q

Test used to differentiate staph from strep

A

Catalase test

SAU - positive

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7
Q

T or F
Staphylococcus epidermidis
- Susceptible to NOVOBIOCIN

A

True

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8
Q

Test to differentiate SAU from Cons

A

Coagulase

SAU - coagulase +

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9
Q

Resistant to 5 ug NOVOBIOCIN

A

Staphylococcus saprophyticus

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10
Q

T OR F

6mm zone size - means resistant or no zone of inhibition

A

TRUE

- True for staphylococcus saprophyticus

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11
Q

CNA

- Disrupts cell membrane of gram (-)

A

Colistin

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12
Q

CNA

- Blocks DNA replication and membrane of gram negative

A

Nalidixic

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13
Q

What makes MSA selective and differential?

A

Selective - presence of high salt concentration (7.5%)

Differential - presence of mannitol and the indicator phenol red

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14
Q

MSA

  1. SAU color
  2. S. epidermidis and S. saprophyticus color
A

SAU - pink (acid change)

S epi and S sap - yellow (no color change)

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15
Q

T or F

Micrococcus luteus and Enterococcus can also produce yellow colonies in MSA

A

True

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16
Q

To Differentiate S. saprophyticus from S. aureus

A

Coagulase test

17
Q

To Differentiate Staphylococcus and Micrococcus

A

Modified oxidase

18
Q

To differentiate Staphylococcus from Enterococcus

A

Catalase test

19
Q

Chromogenic agar

  1. Staphylococcus aureus color
  2. Staphylococcus saprophyticus
  3. Staphylococcus epidermidis
A
  1. SAU - pink
  2. Staph - turquoise
  3. Epidermidis - partially or completely inhibited
20
Q

Pigment responsible for golden yellow color of SAU

A

Staphyloxanthin

21
Q

Positive result and indication:

  1. Catalase test
  2. Coagulase test
  3. Dnase test
  4. Novobiocin susceptibility test
  5. Germ tube test
A
  1. Effervescence or bubbling - positive for staph
  2. Coagulase test - clot and gel (slide and tube) - detect bound and free
    - positive for sau
  3. DNASE test
    No indicator - clear
    Methyl Green - colorless at ph 7.5
    Toluidine Blue O - Bright pink color
  4. Novobiocin - saphro is resistant (zone < or = 16mm) no zone or less than or equal to 16mm
  5. Germ tube - presence of germ tube indicates candida organism

BACTERIOLOGY LAB (4 decks)

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