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Cell Biology Lab > Midterm reviewer > Flashcards

Midterm reviewer Flashcards

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0
Q

Initial absorbance read from the spectrophotometer was 0.803. After 10 minutes, absorbance reading was 0.773.

Find the percentage dye reduced

A

96.26%

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1
Q

What condition in the experimental set-up as described in the result?

Result: Slower rate of Transport or may inhibit totally

A

1:2 ringer:NaCN (IN) – 1:1 ringer:NaCN (out)

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2
Q

Two Factors affecting the separation of pigments from each other in the stationary phase during color development in chromatography

A

Polarity of pigments

Solubility of the pigment in solvent used

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3
Q

Which particular structure in chloroplast does the light reaction take place in photosynthesis?

A

Thylakoid

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4
Q

Which particular structure in chloroplast does the dark reaction take place in photosynthesis?

A

Stroma

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5
Q

Strips of filter papers used in chromatography procedure is in what phase?

A

Stationary

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6
Q

9:1 petroleum ether solution used in chromatography procedure is in what phase?

A

Mobile

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7
Q

Chlorophyll a in Photosystem I absorbs light with a wavelength of 700 nm and is also known as _____.

A

P700

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8
Q

Na + - K+ pump

-passive transport
or -active transport

A

Active transport

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9
Q

_____ states that the absorption of light by a substance is proportional to its concentration in solution.

A

Beer-Lambert Law

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10
Q

Which molecule in the electron transport chain during the Hill reaction in photosynthesis does DCPIP replaces as an artificial electron acceptor in the experiment? _____

A

NADP

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11
Q

Facilitated diffusion
-passive transport
or -active transport

A

Passive

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12
Q

Give the condition in the experimental set-up as described in the result.
Result: Water moves in as Na+ enters the cell

A

Ringer (IN) – Ringer(OUT)

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13
Q

_____ was the substance used in the experiment which inhibited the reaction when chloroplasts are exposed to light.

A

DCMU

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14
Q

Give the condition in the experimental set-up as described in the result.
Result: . No net change on the rate of transport

A

Empty (IN) – Ringer (OUT)

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15
Q

In vivo, which 2 molecules does light energy become converted into during the light reaction phase? _____ and _____

A

NADPH

ATP

16
Q

_____ is the wavelength at which the absorption reading exhibits the highest value.

A

Absorption maximum of a certain substance

17
Q

When the same color in the strips were cut and grouped together, placed in a tube containing acetone, shaken for 5-10 minutes, the product is a partially purified pigment. This procedure is called _____.

A

Elution of pigments

18
Q

Osmosis

-passive transport
or -active transport

A

Passive

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