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bot 340 midterm > Midterm l 1+2 > Flashcards

Midterm l 1+2 Flashcards

1
Q

6 plant life processes

A

primary producers; growth > motility to obtain resources; structurally reinforced; transport systems; mechanisms to avoid dessication; plants develop from embryos

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2
Q

what is the meristem

A

localized regions of ongoing cell division. enable growth

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3
Q

difference between primary and secondary plasmodesmata?

A 
primary = ER membrane maintained thru CW as cell splits
secondary = metabolism digests CW to get ER thru to other cell.
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4
Q

3 major tissue systems

A

dermal; ground; vascular

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5
Q

tissue type within dermal tissue system?

A

epidermis

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6
Q

tissue type within ground tissue system

A

cortex, pith, mesophyll

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7
Q

tissue type within vascular tissue system

A

xylem + phloem

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8
Q

typical cell types in epidermis?

A

epidermal; guard, gland; root haris; trichomes

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9
Q

typical cell types in cortex/pith/ mesophyll

A

parencyma, collenchyma, sclerenchyma

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10
Q

typical cell types in xylem?

A

vessel elements, tracheids. parenchyma, fibers

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11
Q

typical cell types in phloem

A

sieve tube elements, companion cells, parenchyma, fibers + sclereids

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12
Q

function of epidermal cell?

A

protection

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13
Q

function of guard cell?

A

air +water exchange

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14
Q

function of gland cell

A

air +water exchange

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15
Q

function of root hairs/trichomes

A

uptake, protection

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16
Q

function of parenchyma

A

matrix, metabolism, storage, secretion, photosynthesis, transport

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17
Q

function of collenchyma

A

support of growing tissue

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18
Q

function of sclerenchyma

A

support of non-growing tissue

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19
Q

function of vessel elements +tracheids

A

water conduction

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20
Q

function of parenchyma

A

matrix, metabolism, storage, secretion, photosynthesis, transport

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21
Q

function of fibers

A

support

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22
Q

function of sieve tube elements

A

carbohydrate transport

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23
Q

function of companion cells

A

work in tandem with sieve tube elements

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24
Q

function of fibers, sclereids

A

support

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25
Q

function of waterprood outer cuticel?

A

physical barrier for penetration of viruses/predators

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26
Q

what are stomatal apertures controlled by?

A

guard cells

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27
Q

pattern of pavement cells? what tissue system do they belong to?

A

jigsaw puzzle - tight pack.

dermal tissue

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28
Q

what is foudn on lower epidermis

A

guard cell, stomata, pavement cells + cuticle.

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29
Q

functino of guard cells?

A

regulate gas exchange by changing their shape, depending on whether they are turgid or not

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30
Q

guard cells are symplastically isolated - why?

A

because if they were connected to plasmodesmata + symplast they would have very easy access to water + water would be very easily lost

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31
Q

function of trichomes?

A

protect against pests + water loss. synthesize metabolites + may deposit molecules

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32
Q

gland cells function?

A

secrete products onto leaf

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33
Q

root hairs functin? what tissue system?

A

major site of absorption of water + nutrients

–> dermal tissue

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34
Q

parenchyma cells - make up ?

A

make up bulk of plant. metabolic workhorse.

capacity to continue cell division + differentiate into other ground + vascular tissues

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35
Q

collenchyma primary cell walls

A

thickened. involved in reinforcement - important in herbacious not woody plants. can continue to elongate thru maturity.

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36
Q

sclerenchyma 2-ary cw

A

hardened with lignin. dead @ maturity. derive from parenchyma + develop in response to enviro stress

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37
Q

fibers - function + tension?

A

support structure in ground tissue + vascular tissue. high tensile strength

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38
Q

why are xylem vessel elements important + better than tracheids?

A

can bypass xylem vessel in case of rupture or embolism.

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39
Q

phloem conduction

A 
sieve cell (gymnosperm) sieve tube element ( angiosperm)
--> living at maturity therefore needs metabolic activity.

photosynthetic compounds to roots + flowers/seeds.

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40
Q

sieve elements in angiosperms

- organelles? metabolic activity?

A

lost organelles, dont have mechanisms to maintain metabolic activity, connected by plasmodesmata btw companion and sieve tube

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41
Q

function of companion cell, parenchyma cell + connection via plasmodesmata and sieve tubes?

A

help load/unload photosynthates. metabolically dependent on companion cells (angio)

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42
Q

organization of vascular tissue?

A

phloem outside, xylem inside.

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43
Q

function of vascular cambium in stem?

A

allows for secondary growth

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44
Q

stem organization - eudicot vs monocot

A 
eudicot = arranged in bundles, vascular cambium separates phloem from xylem.
monocot = vascular bundles scattered throughout
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45
Q

root vascular tissue organization?

A

single central vascular cylinder. cross of cylem, 4 pockets of primary phloem. vascular cmabium separates, parenchyma pericycle surrounds - may differentiate to 2-ary root development.

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46
Q

vascular organization of leaf

A

vascular bundles in spongy mesophyll - xylem is upper, phloem lower. protected by parenchyma.

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47
Q

9 functions of cell wall?

A

mechanical strength; adhere cells together; control cell shape + expansion; that resists collapse; sensory proteins; diffusion resistance; armour; cuticle

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48
Q

how does cw provide mechanical strength?

A

pectin = tensile strength, still needs flexibility tho. also ensures xylem doesnt collapse - facilitates water transport.

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49
Q

how does cw control cell shape + expansion

A

dictates plant development + morphogenesis = determines growth + polarity.
limits max volume of cell + allows high cell turgor pressure to develop.

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50
Q

sensory proteins in cw?

A

protrude into cell wall space, enable perception of internal + external cues

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51
Q

diffusion resistance as cw function?

A

governs movement thru apoplast - regulates water

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52
Q

armour function os cw?

A

structural + chemical barrier

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53
Q

cuticle function of cell wall?

A

hydrophobic barrier - barrier to water loss, pests, pathogens.

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54
Q

varied architecture in CW - parenchyma vs epidermal cells?

asymmetrical?

A

p = thin, epi = thick. thick i xylem, phloem fibers, sclerenchyma too.
may be asymmetrical thickened like in guard cells or have structural features like pits.

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55
Q

primary cell wall classification

A

young, growing cells. outside = middle lamella = thin pectin-rich layer that cements cells together.
thin + architecturally simple

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56
Q

secondary cell wall classification

A

non-growing mature cells. formed between PM and 1-ary cw after cell enlargement.
- cellulose+lignin. less water, maybe waterproof. very strong. may be highly specialized.

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57
Q

composition of 1-ary cell wall

A

cellulose mf embedded in polysaccharide matrix

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58
Q

where is there highler density of pectin?

A

middle lamella - adhesion btw cell walls

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59
Q

name cw polysaccharides

A

cellulose mf, hemicellulose, pectin matrix, callose, non-enzymatic protein, lignin (mostly 2-ary)

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60
Q

what is callose?

A

forms agregates in phloem to repair or seal hole.

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61
Q

cellulose in 1-ary cw

A

network, crosslinked with matrix polysacch.

mf = ribbones. insoluble in water, high tensile strength + resistant to enzymatic degradation

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62
Q

cellulose chain made of?

A

b (1,4)-d-glucan chain

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63
Q

what is cellulose repeating unit?

A

cellobiose, a b-(1-4)-linked -d- glucose disaccharide

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64
Q

cellulose similar to starch how? different how?

A

glucose polymer. bonds are different = diff properties. every 2nd glucose moleculee flipped to make blink= linear molecule

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65
Q

microfibrils composed how?

A

overlapping b(1-4)-d-glucan chains. arranged in parallel. h-bonds btw neighbouring chains. = v strong - prevent degradation.

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66
Q

biosynthesis of cellulose – in membrane

A

hexameric (6 subunit( rosette - complex contains cellulose synthase - uses MT of skeletal system to deposit cellulose on the outside. cellulose deposited in mf form - not alone bc alone is not rigid.

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67
Q

mutation in rosette - higher temp sensitive or

A

more heat = mutated rosettes. blow up like balloon rather than proper structure. affect cellulose synthase + affect ability of cell expansion

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68
Q

discuss CESA - cellulose synthase

A

membrane- spanning.
cytosolic catalytic domain transfers glucose from donor to glucan chain.
CESA is multigene. many diff forms of the complex.

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69
Q

microfibril orientation influences growth directionality of cells

A

cellulose mf perpendicular to axis of cell expansion in growing tissue. - facilitates directional growth = high tensile strength of mf, but can pull mf apart and deposit cw in between = growth.

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70
Q

how is cellulose mf deposition regulated?

A

cellulose synthase commplex moves across membrane + leaves trail of cellolose parallel to Cw.
== anisotopic cell expansion

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71
Q

difference btw isotropic and anisotropic cell expansion

A

iso = randomly oriented mf = expand in all directions = circle
aniso - transverse mf. = expand at right angles to mf orientation.

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72
Q

alignment hypothesis of cellulose deposition

A

assoc with underlying cortical MT. MT guide CSC as they synthesize cellulose

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73
Q

what would occur if cell synthase didnt have MT?

A

lose ability to track + place cellulose. lose directionality/pattern of deposition

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74
Q

2 components of matrix polysaccharides

A

hemicellulose + pectin

- nonlinear, branched polysacch

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75
Q

matrix polysacch synthesized whered?

A

golgi + secreted into apoplast via vesicles

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76
Q

structure of hemicellulose?

A

long, linear polusacch with short side branch. ex: xyloglucan. b(1-4)-d-glucose chain w xylose branches

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77
Q

consequence of hemicellulose branches?

A

can’t do tight packing.

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78
Q

what kind of plants have high density of xyloglucans?

A

dicots = angiosperms.

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79
Q

two models of hemiceullulose binding to cellulose

A

tethered network model

+ biomechanical junction

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80
Q

what is the tethered network model?

A

hemicellulose binding to cellulose == early model suggests xyloglucans coated cellulose = cross bridge = tether mf together.

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81
Q

what did tethered network model miss?

A
  1. that mf not coated with xyloglucans. 2. mutatants that don’t synthesize xyloglucans show normal growth pattern.
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82
Q

what is the biomechanical junction model?

A

hemicellulose bind to cellulose == better model suggest there are regions of close association btw mfs. hemicellulose mediates this region = double sided sticky tape

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83
Q

what are pectins?

A

highly branched, heterogeneous polysacch. = galatouronic acid backbone.

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84
Q

what are pectins important for?

A

cell adhesion.

hold 40% of the mass in cell wall, and 60% of the water in the cell wall.

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85
Q

where are pectins found?

A

middle lamella - cell adhesion.

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86
Q

how does pectin gel form?

A

charged - so highly hydrophilic. creates gel = no flow to water, enables flex but prevents flow.

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87
Q

what does the amount of pectin determine?

A

the amount of flow/porosity of the gel

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88
Q

function of pectin?

A

prevent aggregation + prevent collapse of 3d structure

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89
Q

pectin - branches, bonding & effect on gel

A

less branching = more ordered gel = smaller pore size

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90
Q

how Ca2+ effects pectin gel formation

A

Ca2+ creates ionic bridges = increase methylation.

-> methylation of carboxyl groups on pectin = prevent bridges = lless rigid.

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91
Q

what is callose?

A

b(1,3)-glucan. not linear = can aggregate + plug holes. sticky properties.

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92
Q

where is callose synthesized?

A

PM, deposited btw PM and CW.

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93
Q

what is callose for?

A

pollen development, induced by wounding, deposited at Plasmodesmata to regulate cell-cell movement

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94
Q

callose function in plasmodesmal aperture

A

deposited in CW collar = control open/closure of PD. easily broken down/replaced

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95
Q

proteins in primary cw. functino?

A

2-10% non-enzymatic proteins. function? maybe cell-cell adhesion, cell differentiation

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96
Q

where are 2-ary cw deposited? functin?

A

around mature cells, between pm and 1-ary cw. add strength, compression resistance to stems.
- each layer has different macrofibril orientations

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97
Q

what are macrofibrils?

A

2-ary cw tightly packed into macrofibrils. coated w hemicellulose = less branched more rigid. bound by lignin = waterproof

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98
Q

1-ary vs 2-ary cw

A

1 : large variation, lots of pectin, highly hydrated, can be modified with cell growth

2: higher cellulose, hemicellulose has less branching, lignin = waterproof + mechanical support

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99
Q

lignin function

A

structurally rigid; transport (strength for xylem); hydrophobic walls (casparian strip - regulate water flow into pericycle); defense

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100
Q

what is zone of dehiscence? relates to seed dispersal?

A

on zone surface, lignin assoc with surface of valves. when dehydrated on top, lignin on bottom stays rigid= open valve = seeds spring out + disperse.

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101
Q

what are the building blocks of lignin + what are building blocks derived from?

A

monolignols - derived from phenylalanine

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102
Q

how is lignin synthesized?

A

monolignol secreted, random polymerization of monolignols by oxidative coupling. lignin displaces water

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103
Q

function of water in plants

A

participate in biochemical rxn, affect ion absorption, turgor, movement, role in phenotypic expression ( root morphology, leaf morphology, stomata

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104
Q

most limiting factor in agriculture?

A

water.

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105
Q

CO2 absorptio effect on water?

A

loss of water. transpiration = stomata open bc need CO2 at same time, water diffuses out.

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106
Q

what is chemical potential?

A

measure of the free energy per mole of a substance.more concentrated = more free energy.

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107
Q

diff values of mew (u) - chemical potential?

A 
(-) = spontaneous.
(+) = requires energy input.
0 = at equilibrium
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108
Q

chemical potential of water measures?

A

free energy per mole of water within system. . proportional to concentration of water.

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109
Q

if difference in water potential is (-)?

A

water will flow from higher chemical potential to lower chemical potential

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110
Q

what affects water movement?

A

physical + chemical properties; mode of water transprot - diffusion/mass flow

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111
Q

properties of water due to polarity

A

excellent solvent (reduce electrostatic interaction); liquid at “biological” temps; water has high specific heat + thermal conductivity (buffer temp fluctuation); high latent heat of vaporization (energy required to shift from liquid to gas phase); highly cohesive ( attraction btw water molecules. surface-tension = energy required to increase air around water molecule; highly adhesive to surfaces); high tensile strength (supports large (-) hydrostatic pressue; incompressible; allow internal hydrostatic pressure in plants); low viscosity; max density at 4C

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112
Q

water movement by bulk flow

A

move when external force is applied; long distance travel; independent of concentrations; sensitive to change in radius of transport conduit

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113
Q

water movement by diffusion

A

directional movement down free energy gradietn. high -> low.

diffusion affected by any factor that influences chemical potential.

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114
Q

ficks law

A 
js = d + change in c / change in x
-> js is flux density (rate of diffusion)
d = diffusion coefficient
c = concentration
x = distance
(-) = diffusion from high -> low
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115
Q

what does diffusion coefficient mean?

A

describes how easily substance (s) moves through a medium.

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116
Q

what 3 factors effect diffusion coefficient?

A

substance characteristics (larger molecule = slower); medium (thru air faster than water); temperature (diffuse faster at higher temp)

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117
Q

rate of diffusion inversely proportional to?

A

distance over which movement occurs. only important over sort distances.

118
Q

define osmosis

A

movement of water across a selectively-permeable membrane. other solutes restricted.

119
Q

factors + contributors to osmosis?

A

diffusion, bulk flow + pressure (counteracts movement of water)

120
Q

how is water potential related to chemical potential?

A

chemical potential of water = specific, can predict outcome of processes

121
Q

how do plants control water movement?

A

indirectly. move ions/solutes to alter the free energy of water.

122
Q

2 major components of water potential

A

osmotic potential + pressure potential.

123
Q

what is water potential of pure water?

factors that effect water do what to its value?

A

0 ; make more negative, lower water potential

124
Q

first law of thermodynamics?

A

conservation of energy - energy cannot be created or destroyed.

125
Q

second law of thermodynamics?

A

total entropy always increases, no outside input = spontaneous = increase entropy

126
Q

how does entropy relate to thermal energy?

A

amount of isothermically unavailable energy in system = TS ( T is absolute temp; S - entropy.
related to energy of molecular motion. when temp is 0, entropy is 0. more free = more chaotic - greater entropy. hotter temp - increase entropy

127
Q

what doe gibbs free energy measure?

A

max energy available for conversion to work.

128
Q

change in free energy - when (-); when (+)

A
  • : spontaneous

+: endergonic. requires energy input

129
Q

value of change in free energy depends on 2 factors

A

standard free energy change for reaction; concentration of reactants + products

130
Q

at equilibrium no work can be accomplished, why?

A

free energy at minimum and entropy at maximum.

131
Q

further the mass-action ratio is displaced form equilibrium (Keq), what happens to reactan?

A

greater free energy for given change in reactant to product.
further a rxn from equilibrium, greater capactiry to do work

132
Q

as reactant reaches equlibrium what happens to deltaG

A

deltaG is negative and free energy is available to do work.

133
Q

when [products]/[reactants] is greater than less than Keq

A

greater than = positive; less than = negative = spontaneous

134
Q

many factors that affect water potential

A

solute potential; pressure potential; temperature, concentration, gravity, water adsorption to solid surface

135
Q

solutes in water affect water potential (+/-)

A

mole fraction of water is reduced when solutes are added. (-)

136
Q

what is pressure potential due to?

A

hydrostatic pressure on free energy of water. measured relative to ambient pressure for pure water in an open state, which is set to zero.

137
Q

pressure potential + effect on water potential

A

+ = raise Y; ex: water influx - positive pressure on cw - turgor.
- = lower Y; ex: xylem conduits when water column under tension

138
Q

what is matric potential?

A

from adsorption of water to solid surfaces of cell, soil etc.

  • > greatest effect on dry states.
  • > matric potential = negligible
139
Q

temperature potential

A

driving force in diffusion. but effect is quite small overall

140
Q

concentration potential

A

contributor to chemical potential gradients in most systems.

changes in water concentration are negligible. concentration potential is negigible in plants

141
Q

gravitational potential

A

causes water to move downward - depends on height of water + density of water + acceleration due to gravity.
-> negligible at cell level

142
Q

why is measuring water potential useful?

A

measured experimentally; relevant to soil-plant-atmosphere system; gradients in water potential describe passive flow of water; measure physiological status (water stress - lose turgor - decrease water potential)

143
Q

vacuole + water potential

A

hold dissolved solutes + 50-80% of cellular water = major contributor to water potential.

144
Q

plants gain water = ? pressure

A

turgor. equal + opposite wall pressure/water pressure

145
Q

cell loses water called?

A

flaccid

146
Q

hypotonic solution vs hypertonic

A

hypo = cell gain water bc water potential in cell more negative than outside.

hyper = cell lose water = plasmolysis bc cell more + than solution

147
Q

vacuole + water potentiall

A

greatest contribution to water potential bc occupies majority of cell volume.
-> isolated from cytosol by tonoplast

148
Q

why is movement of water through biological membranes greater than dissuion thru lipid bilayer?

A

water can diffuse thru phospholipid molecules, but ALSO transferred across membrane through Aquaporins.

149
Q

aquaporins - water, energy, direction. gates? influence on conductivity

A

water-selective (prefers water but moves small neutral particles thru); no energy inputs. provide a channel for diffusion. bidirectional - based on water potential.
gated= can be selectively opened/closed (due to pH, cation concentration, protein phosphorylation); role in hydraulic conductance - measure of membrane permeability to water.

150
Q

where are aquaporins found?

A

PM and tonoplast. water movement in out of membranes.

151
Q

how does water move from soil to top of tree?

A

diffusion would not occur because rate is inverse to distance. greater distance = decreased rate of diffusion.

152
Q

what is a “plant’s conundrum”

A

absorption of atmospheric CO2 thru stomata exposes plant to water loss while stomata are open.

153
Q

describe water movement thru plants. rlies on ?

A

relies on continuous connection of water from soil -> roots -> stem -> leaves. high tensile strength + cohesiveess of water contribute.

154
Q

how water is transpired?

A

water vapour concentration = plant —> atmosphere.

155
Q

how soil particles act with water?

A

soil particle size determines characteristics of soil ie retention of water. large pores= more water fills = field capacity.

156
Q

dry soil vs clay re: water

A

dry soil will drain water quicker, whereas clay will hold more water. dry - more suceptible to dehydration, clay takes a lot longer to let go of water

157
Q

how surface tension works in soil?

A

surface tension is increased around curved surfaces. as more water exaporates, the tension between the soil particles and water increases and water potential decreases.

158
Q

water potential in soil, how does water travel to roots?

A

water travels through bulk flow from soil, to root-soil.
gravitational potential plays an important role. solute potential is negligible because of dilute solutions. pressure potentials are usually negative; with greater surface tension as soil dries = decreases pressure potential

159
Q

flow of water + soil-water content. what is soil-water content?

A

water moves from greater soil water content to lower soil water content.
greater = larger water-filled spaces, less surface tension, greater (less neg) water pressure potential.

-> plants tak eup water, deplete water from soil, keeps water pressure potential of soil near roots more negative = induces bulk flow

160
Q

2 things that have greatest effect on water flow thru soil

A
  1. pressure potential.

2. hydraulic conductivity - larger interstitial space = higher hydraulic conductivity

161
Q

what is permanent wilting percentage? how does this correspond to soil-water potential?

A
  • plant cannot maintain turgor pressure even if water loss is minimized.
  • -> due to water potential of soil. if lower hydraulic conductivity = more resistance, water cant get thru soil to roots. = decrease water potential of soil. but need greater water potential of soil compared to water potential of root cells to have flow of water into roots.
162
Q

4 functions of roots

A
  1. anchoring plant; 2. store carbs + molecules; 3. synthesis of hormones + metabolites; 4. uptake + transport of water + minerals.
163
Q

plant root exploration facilitates ?

where does water uptake mainly occur?

A

water uptake.

mainly occur at growing tips of roots, where root hairs are. non-growing regions are lignified to prevent water +nutrients to flow there. focus on “feeding” growing area. also helps establish water potential in plant

164
Q

how does lignification of older roots help to establish water potential?

A

lignified = water-resistant. low water concentration higher up in root = lower water potential, pulls water up, as well xylem takes water away from older roots bc of pressure potential drives water uptake in young roots.

165
Q

how do root hairs contribute to water uptake?

A

increase explored volume of soil + can penetrate small pores (access areas big roots couldnt)

166
Q

3 ways of radial movement of water

A

apoplast: cell wall space, continuous with root’s outside enviro.
symplast: cytoplasmic connections via plasmodesmata
transmembrane: cell:cell, aquaporins/difusion thru membrane

167
Q

what is Casparian strip? what’s its function?

A

casparian strip = lignified + suberized cell wall at epidermis. function: forces water to pass thru symplasm before entering xylem. requires aquaporins for water to flow from apoplast to symplast, thru casparian strip to vascular bundle.

168
Q

xylem loading

A

concentration of ions in xylem ; get in thru selective transport through endodermis = greater solute concentration = lower solute potential inside xylem = lower water potential in xylem drives water in.

169
Q

root pressure = positive pressure.

A

water flow into xylem. increase water in pushes water up.

170
Q

what is guttation?

A

xylem sap/water exudes from plant when positive pressure pushes water up xylem due to high water potential of soil and low rates of transpiration. - water being pushed up bc high positive pressure &xylem solute loading. no transpiration, water not lost so xylem extrudes thru pores of plant.

171
Q

what evidence shows that positive root pressure is not the main mechanism of xylem transport?

A

root pressure is rarely more than 0.1 MPa (raises only short distance) & transpiring plants show xylem is under negative pressure (tension)

172
Q

evidence that shows atmospheric pressure/suction is not the mechanism for xylem transport?

A

under standard conditions, water could be raised 10 m, however, not standard conductions; water colum not subject to atmospheric pressure because they are in equilibrium

173
Q

why capillarity is not the mechanism for xylem transport?

A

water under curved surface develops negative pressure potential however, radius of xylem wouldn’t contribute much to pulling water up

174
Q

what is cohesion-tension theory?

A

water evaporation from cell walls of mesophyll cells is driving force. water evaporates = tension (closer to surface of cells), cohesive network of water pulled by this tension

175
Q

fourcell types in tracheary elements

A

tracheids, vessel elements (angiosperms); fibers (sclerenchyma, elongated + lignified - dead at maturity); parenchyma (storage, living)

176
Q

how tracheids + vessel elements contirbute to moving water

A

lower resistance to moving water. open tubes.

-> extensive 2-ary cell wall = compression strength = negative pressure = decreases pressure potential

177
Q

function of lignin?

A

strength + increase hydrophobicity = less interaction btw cw and water = greater flow

178
Q

tracheids pits - how do they work?

A

plasmodesmata connection between adjacent tracheids. thin filters.

179
Q

embolism - how?

A

when pressure potential more negative than vapour pressure = water vapour bubbles + expands. low pressure potential and high water vapour pressure - water turns to water vapour instantly = bubble. embolism

180
Q

vessel element - found where? assembled how?

A

in angiosperm (absent in most gymnosperm), shorter + wider. assembled end-to-end. separated by perforation plates (open hole or drain - low friction, high flow)

181
Q

radius of conduit

A

-> slight increase in diameter = great increase in flow.

but, increased risk of cavitation (xylem break) - vessel elements in contact = easily spread embolism

182
Q

resistance to water movement thru xylem; pressure required for xylem resistance. gravity pressure?

A

resistance thru xylem lowered bc vessel elements + tracheids. need 0.01MPa/m to overcome xylem resistance. but, water potential due to gravity is 1 MPa/100m ==> need 2Mpa to move water up

183
Q

how transpiration is the driving force for water up the xylemm

A

water on surfaces of mesophyll cells; water vapour in intercell space higher than in air outside = diffuses + exits leaf. water liquid + vapour in equilibrium. as vapour diffuses out, liquid on surface of cell vapourizes. as it vapourizes, surface tension of water induces negative pressure&pulls water up xylem.

184
Q

how is water loss reduced?

A

presence of waes + cuticl.

185
Q

how much of water lost thru transpiration? how to regulate?

A

95%. regulate by open/closing stomata.

186
Q

driving force for transpiration?

A

difference in [water vapour] inside + outside the leaf.
saturation water vapour content increases with leaf temp. = warmer air holds more water, pull water vapour from leaf even when v humid.

187
Q

water vapour diffusion proportional to?

A

difference in [water vapour] inside vs outside. inversely proportional to length of diffusion path.

188
Q

diffusion coefficient (Ds) influenced by?

A
  • leaf stomatal resistance = stomatal pore diameter can be regulated
  • boundary layer resistance = trapped air close to surface, increase distance of water movement thru stomata to decrease water loss.
189
Q

what is the boundary layer resistance? what affects its thickness?

A

boundary layer resistance = trapped air close to surface, increase distance of water movement thru stomata to decrease water loss.

  • > leaf shape (leaf curls in - increase boundary layer bc air trapped inside roll), size, stomatal architecture (pore, or sunken stomata -trapped layer of air = increase path)(stomata cluster - increase overlap = increase boundary layer, presence of leaf hairs (increase boundary layer bc trap air) + wind speed.
  • > wind reduces boudnary layer = more water loss
190
Q

stomata regulation + transpiration

A
  • cant modify water vapour gradient dirctly. regulate stomatal aperture to regulate resistance.
191
Q

what is stomatal aperture determined by?

A

changes in turgor pressure of guard cells.

-> open = turgor pressure increase

192
Q

two main types of guard cells

A

dumb bell = grasses. pore is slit btw bulbous guard cells, flanked by subsidiary cells.

  • kidney: pore forms in center. may/may not have subsidiary cells
193
Q

dumb-bell guard cells = cw, cytosol?

A

cw is thicker in centre, like q-tips. cytosol restricted to bulbous ends.

194
Q

kidney-shape guard cell

A

thicker inner + outer lateral cw. thin CW adjacent to epidermal cells == thicker on inner surface of pore

195
Q

what determines cell shape changes during fluctuating cell turgor?

A

cellulose microfibril alignment. - expansion perpendicular to orientation of cellulose.

dumbbell: increase water in cytosol (q-tip ends) - long inner parts separate + slit widens

kidney= inner pore has thicker cellulose. as cell swells, thicker layer inside cant move, rather bends + opens pore.

196
Q

stomata open when?

A

in response to light, or high [co2], leaf water status, stress signals.
light dominant = restricts water loss at night bc no CO2 fixing bc no light = no water lost.

197
Q

2 main stimulations of stomatal opening

A

photosynthesis in guard cell chloroplast & blue light

198
Q

how does blue light cause stomatal opening?

A

establishes PM H+ pump = inward elecrochemical gradient, K+ enters down gradient, cl- and malate follow due to charge. = solute loading in guard cell = water follows = increase turgor = open stomata.

199
Q

what is hydropassive closure of stomata?

A

rapid water loss from guard cell = loss of trugor

200
Q

what is hydroactive closure?

A

water stress stimulates synthesis of ABA => influx Ca2+, inhibits H+ pump & K+ influx. stimulate ion effluc = watter follows out to decrease turgor + close stomatal conductance

201
Q

how does xylem not collapse even under extreme negative pressure

A

sufficiently lignified to prevent collapse

202
Q

how water in xylem maintained in metastable state?

A

cohesion + adhesion properties of water = decrease water vapour; xylem structure minimizing nucleation sites (air bubbles)

203
Q

define embolism

-> limited how?

A

gas-filled obstruction of the conduit that breaks the water column.
pits act to limit movement of bubbles, easier to move bubble thru vesssel element perforated plates. root endodermis limits bubble entry

204
Q

how do plants minimize xylem cavitation? conifer

A

conifer pit membranes = central torus (plug) + flexible pit membrane (margo AKA strings)
when pressure differntials occur, torus lodges into side with lowest pressure = valve to prevent embolism

205
Q

minimie xylem cavitation - angiosperm

A

lack tori, smaller pores in pit membrane. block movement of water. gas bubbles cant get thru readily - decrease cavitation

206
Q

how adaptation helps w cavitation

A

adapted to dry enviro = less suceptible to cavitation. => minimize spread of embolism (outgrowths of xylem parenchyma seal xylem vessels) repair embolism

207
Q

measure susceptibiility of cavitation

A

centrifuge attachment - induce large (-) pressure to simulate moist stress. cavitation occurs after xylem conduits blocked = measure hydraulic conductance of stem. more (-) pressure potential = more likely to develop embolism

208
Q

how to restore water flow after cavitation

A
  1. those w 2-ary growth => generate new tracheary conduits ( replace, spring = new xylem growth)
  2. refill cavitated conduits: gass dissolve into liquid if pressure is sufficient (+ pressure potential) night = decrease difference in pressure potential. root pressure continues to load xylem = + pressure.

-> refill can also occur if xylem remains under tension (unsure how).
freezing + dehydration cause embolism, water thaws = positive hydrostatic pressure = dissolve embolism

209
Q

most abundant chemical elements in plants?

A

O,C,H.

210
Q

what is mineral nutrition?

A

study of how plants obtain + use mineral nutrients

211
Q

3 main mineral nutrients, acquired how?

A

N, P, K : acquired as inorganic ions from soil

212
Q

why is mineral nutrition important?

A

availability limits plant growth + primary productivity.

213
Q

features of essential mineral nutrients?

A

absence = cannot complete normal life cycle

element is part of essential molecule or must be involved in biological process.

214
Q

macronutrients vs micronutrients.

A

macro: relatively high concen. [ >10 mmol/kg)
micro: low concen. [<10 mmol/kg]

215
Q

examples of macronutrients

A

N , K, P, Ca, Mg, S, Si

216
Q

micronutrients

A

Cl, Fe, Mn, Na, Cu

217
Q

how to determine mineral nutrient essentiality?

A

grow plants in solution = containing inorganic salts; simulate soil nutrient enviro.

218
Q

what is deficiency zone?

A

range of [mineral nutrient] below critical, to where plant growth is just observed. no longer limiting.

219
Q

what is adequate zone?

A

range of [mineral nutrients] beyond which addition of nutrient doesnt increase growth/yield.

220
Q

what is critical concentration?

A

minimum concentration of mineral nutrient correlated with max growth/yield.

221
Q

what is toxic zone?

A

range of [ mineral nutrient] in excess of adequate zone = growth/yield declines. toxic

222
Q

deficiency in nutrient display 2 visual symptoms

A

chlorosis: loss of chlorophyll pigment.
necrosis: death of cell + tissue

223
Q

describe phosphate deficiency

A

purple margins on young leaves

224
Q

describe potassium deficiency

A

firing on margins of older leaves.

225
Q

describe nitrogen deficiency

A

yellowing in center of older leaves

226
Q

why is there a problem with visual symptoms?

A

may be multiple deficiencies; deficiency in 1 nutrient, toxicity in another; disease imitates mineral deficiency

227
Q

mineral deficiency depend on 2 things

A
  1. nutrient function. 2. mobility of nutrient.

phloem-mobile: affect older.
phloem- immobile affect yougner.

228
Q

mobile nutrients?

A

N, P, Mg, K, Cl, Na, Z

229
Q

immobile nutrients

A

Ca, S, Fe, Cu

230
Q

forms of N available?

requirement? role? deficiency symptom

A

ammonium, nitrate. require 1000 mmol/kg. most required. role = synthesis of macromolecules. (-): inhibit plant growth, chlorosis of old leaves, accumulation of anthocyanin

231
Q

form of P available? requirement? role? deficiency symptom?

A

PO4(3-); require: 30mmol/kg. role: sugar-phosphates in metabolism, ATP. deficiency: dwarf, stunted dark green leaves, anthocyanin, malformation of leave. older first (bc mobile)

232
Q

K form available? requirement? role? deficiency symptom?

A

K+; 250 mmol/kg. primary osmolyte, cofactor in enzymes.

chlorosis + mottling in old leaves. marginal leaf necrosis. older leaves first bc mobilized

233
Q

why i P limiting at such a low requirement?

A

30 mmol/kg. closely assoc w soil. hard to acquire into plant therefore limiting

234
Q

soil features:

A

heterogeneous, vertically stratified, biologically diverse.

235
Q

when are nutrients available to plant in soil?

A

when in soil solution = solution phase.

236
Q

what happens when ion assimilated by plant?

A

creates nutrient depletion zone around root.

237
Q

what are soil colloids?

A

clay particles in soil solution, main reservoir for soil nutrients.

238
Q

role of colloidal fraction depends upon?

A

specific surface area (capacity to hold ions); density of fixed negative charges (more neg charge = more cation attraction + hold)

*dont bind anions. cation binding is exchangable

239
Q

Iyotropic series = ability of colloidal surfaces to bind soil cations follows this trend

A

al > H > Ca > Ca >K = NH4 > Na.

240
Q

factors influencing mineral nutrient delivery to roots

A
  1. soil cation exchange capacity (exchangeable + bind to -. dynamic equilibrium + buffering capacity.
  2. soil pH (decomp of organic matter; ammonium, rain):
  3. water flow
  4. root architecture
  5. root-microbe interactions
241
Q

what influence pH and cation exchange?

A

rhizosphere: immediate microenvironment surrounding the root.

242
Q

how water flow influences mineral nutrient dlivery to roots

A

bulk flow important when soil moisture high + [nutrient] high. (pull soluble to roots by bulk flow)

rate of nutrient uptake exceeds rate of delivery via bulk flow = nutrition depletion zone.

mycorrhizal increase nutrient depletion soil.

243
Q

two components of increased soil pH that relate to mineral nutrient delivery to roots

A

a. regulate solubility: phosphate precipitates by Fe + Al/pH
b. regulating cation exchange, soil acid release bound cations, lost thru leaching. + binds higher binding ability = less avail but more necessary.

244
Q

how does root architecture affect nutrient delivery to roots?

  • > what does root development depend on?
  • -> 3 areas of root development
  • –> influence on root proliferation
A

fibrous ( many branches) vs. taproot (one long branch)
-> responsive to environmental factors, differ in surface area covered. nutrient uptake greatest at root apical zone = most demand.

  • -> depend on root apical meristem. meristematic zone: cells generate root cap + upper tissue.
    • cells in elongation zone - vertical, phloem, xylem cortex produced.
  • – root hairs formed by epidermal cells, in maturation zone first

—> nutrient concentration determines degree of proliferation. denser roots where more nutrients are.

245
Q

how do root-microbes influence mineral delivery to root?

A

rhizosphere modified to enhance nutrient acquisition.

exude organic compounds into rhizosphere = growth of microorganisms, give + get (fixed nitrogen, solubilized nutrients)

246
Q

what is mucigel?

A

mix of root exudates, living + dead microorganisms + soil colloids.

247
Q

what is function of mucigel?

A

protect root cap, lubricate root cap, symbiotic enviro for fungi + N-fixing bacteria (symbiotic); diffusion bridge btw roots + soil, more efficient.

248
Q

what does mycorrhizal symbiosis facilitate?

A

water + nutrient uptake by roots.
exhcnage nutrients for carbs provided by plant.
increase effective root surface area. increase nutrient depletion zone.

249
Q

hyphae of ectomycorrhizal do what?

A

surround root cells, obtain nitrogen from decomposition of soil organic matter. close connection to plant CW = get’s nutrients there.

250
Q

= endomycorrhizae = arbuscular mycorrhizae

A

highly branched, large surface area. vesicles for storage, fast-growing + short-lived. form invaginations in cell membrane.

251
Q

PM function in ion transport?

A

metabolic control of ion uptake. selective; requires energy to move ion thru the root. = xylem loading.
-> larger molecule = hard to get across. same w charged. higher Ds = easier solubility

252
Q

what is fick’s law:

A

rate molecules diffuse btw regions is function of the concentration difference.

253
Q

ion charge interacitons - why is ion permeability hgiher in biological membranes than lipid bilayers.

A

ion charge + attract water = larger water-bound complex. insoluble thru membrane.

higher transport than diffusion = transporter facilitating.

254
Q

two important characteristics of solute transport across membrane

A

accumulation ( concen.) and selectivtiy ( certain ion thru certail channel)

255
Q

role of vacuole in electrochemical gradients

A

accumulates K+ when external conc. are low.

Na+ and Ca2+ pumped into apoplast + vacuole; same w H+. anions taken up too

256
Q

2 components of membrane potentials

A
  1. diffusion potential ( potential due to diffusion)

2. electrogenic transport established by H+-ATPase. (1-ary active transport; generate gradient of H+ movement across PM)

257
Q

effect of PM H+-ATPase

A

change in pH: accumulate H+ in vacuole and apoplast.

  • > electrogenic: net change movement.
  • -> establish proton motive force.
258
Q

speed of transporters:

A

channels: 10^8 ions/sec.
carriers: 100-1000 molecules/sec

259
Q

tissues often have complex kinetics for ion transport - indicates what?

A

indicates presence of more than one type of transport mechanism. = high (low KM - quickly saturable) + low-affinity ( high KM, linear portion) transporters.

260
Q

time-dependent effect on ion transport?

A

saturable = diffusion into cell wall space.

biphasic: cation uptake due to accumulation in apoplasm (rapid, passive) & symplasm (linear, metabolism-dependent)

261
Q

2 regulatory functions of PM H+-ATPase

A

regulates cell turgor of guard cells; regulates cell expansion (auxin needs to be acidified => loosend cw)

262
Q

define energy

A

capacity to cause change

263
Q

define work (mechanical)

A

diplacement against an opposing force

264
Q

define kinetic energy

A

associated with motion and includes thermal energy associated with random motions of atoms or molecules

265
Q

define heat

A

thermal energy in transfer from one object to another

266
Q

define potential energy

A

stored energy that can be converted to other forms of energy to do work

267
Q

work (biological)

A

displacement against forces that living things encounter or generate
- often involves changes in chemical potential energy

268
Q

what is first law of thermodynamics

A

conservation of energy
- in all chemical and physical changes, energy cannot be created or destroyed, only transferred or transformed from one form to another

269
Q

what is second law of thermodynamics

A

total entropy always increases in a closed system.

- spontaneous = no outside input of energy.

270
Q

what is entropy?

A

amount of isothermically unavailable energy in a system. therefore, amount of energy in a system not available to do work.
- related to molecular motion + temperature.
at absolute zero, molecular motion ceases. more molecules are free to more when greater temp - more chaotic.

271
Q

what is gibbs free energy?

A

measure of the maximum energy available for conversion to work.
enthalpy - total heat energy, including work.

272
Q

different signs of change in free energy (+/-)

A

negative: spontaneous. exergonic

positive : non-spontaneous, required energy for reaction to occur. endergonic

273
Q

value of gibbs free energy depends on what? (2)

A

standard free energychange of reaction

concentration of reactants + products

274
Q

what happens to free energy and entropy when at equilibrium?

A

free energy at minimum

entropy at maximum

275
Q

what is chemical potential?

A

partial molal Gibbs free energy

- free energy per mole of substance within a system

276
Q

what are properies of water

A
  • excellent solvent: reduces electrostatin interaction = solubility of ionin and polar molecules is high
  • liquid at “biologial”temps
  • high specific heat + thermal conductivity
  • high latent heat of vaporization: required to move molecules form liquid to gas phase is high
  • highly cohesive: mutual attraction. =surface tension and adhesion
  • high tensile strength. supports large negative hydrostatic pressure, incompressible.
  • low viscosity
  • max density at 4C
277
Q

function of ATP in cellular work

A

couples exergonic reactions to endergonic reactions.
- high stored energy. PO4 repel each other by nature. when unbind lots of energy released
-stores potential energy.
drives endergonic reaction by transfer of phosphate group to reactant - phosphorylated intermediate that is more reactive.

278
Q

effect of phosphorylation of H+-ATPase

+ dephosphorylation?

A

changes proteins conformation, release of bound H+

-protein returns to original, low energy state. binds H+.

279
Q

describe channels

A

charge-lined, water-filled.
passive but specific (pore size +charge)
rapid movement
-often gated in response to external signs.

280
Q

describe carriers

A

no transport pore.
bind solute to be transporter - induces conformational change to shift solute across membrane.
highly selective, slow compared to channel

281
Q

primary active transport?

A

carrier proteins directly use energy source to mediate transport. against electrchemical gradient + unidirectional

282
Q

electrogenic vs electroneutral?

A

electrogenic: net movement of charge across the membrane

electroneutral no net movement of charge

283
Q

secondary active transport?

A

against gradient
coupled to movement oof another solute down its electrochemical gradient.
-may be driven by electrogenic H+ATPase = proton motive force

284
Q

define symport + antiport

A

symport: H+ and solute move in same direction
antiport: H+ and solute move in opposite directions

285
Q

kinetics of solute transport - Vmax and Km

A

Vmax = maximum rate of transport. carriers full, channel at max
Km - 1/2Vmax - binding properties of binding-site (affinity)

286
Q

why do tissues show complex kinetics for ion transport?

A

usually indicates more than one type of transport mechanism.
kinetics predict saturation - high affinity transporters do saturate, but low affinity (slow movement) continue to transport after high affinity maxed out.

287
Q

how time affects complex kinetics

A

becomes saturated over time.. not immediate.

288
Q

PM H+-ATPases

REALLY IMPORTANT

A

maintain electrochemical potentials across membranes, regulate cyosolic pH.
- affects cell turgor (guard cells)
and cell expansion (induced by auxin - initiate cell wall loosening)
-> energize cell nutrient transport across barriers separating symplast from apoplast

289
Q

PM H+-ATPase in guard cells

A

blue light stimulates H+-ATPase activity.
PM hyperpolarized, generates driving force for ion (K+) uptake. decrease solute water potential (malate accumulation) of guard cells and increase guard cell turgor causing stomatal opening

290
Q

how PM H+-ATPase functions

A

N-domain of protein binds nucleotide. = phosphorylation causes conformaitonal change.
- twist head, tension opens other side to extrude H+. no counter-ion movement

bot 340 midterm (6 decks)

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