Midterm Flashcards
Using the coarse focusing knob, which way must it be turned for the stage to move away from the objective?
Clockwise
When you place the “roe” slide on the microscope, what happens when you look through the lenses?
It flips upside down and horizontally
What happens to the magnified image when you move the slide to the left or right?
It moves the opposite way
As magnification increases, does field of view increase or decrease?
Decrease
On the crossed fibers slide, what thread was on top?
Blue
Microbiology is the study of…
Microbes
The “medium” is
the nutrient rich material in which you culture the microorganisms
In the bacterial growth and inhibition lab, what was the medium?
Agar
What is inoculation?
Adding microbes to the medium in which they will grow
What is the aseptic technique?
avoiding contaminating your culture, yourself, and your surroundings
What were the steps in the bacterial inhibition lab?
1) divide petri dish
2) use a swab to obtain a sample from a surface and swab onto one half of petri dish
3) wipe down surface with alcohol
4) swab surface again and rub on other half of petri dish
Hypothesis of Bacterial Growth experiment
If we swab the same area with alcohol, there will be significantly less bacteria growth
4 variables that could affect the outcome of the bacterial growth experiment
Human error
Strength of the alcohol
if someone swabbed before us
amount of alcohol used to clean
What does a control plate control for?
To show what the sample would look like without contamination
How many colonies were on the petri dish after incubation? (on each side) What kind of colonies were they?
28; none; bacterial
