midterm 2 Flashcards
Condenser
focuses light that passes through stage
Field of View
thickness of element in focus
Depth of field
distance between closest and farthest objects that are in focus
Inter pupillary distance
distance between eyepieces
Letter e under micro….
upside down and flipped (180 degrees)
HPD = (LPD x LPM) / HPM
HP - high
LP low
D - diameter of FOV
M = magnification
The LARGER the magnification the….
SMALLER the field of view
inverse relationship
Resolution
smallest distance between two points that can be seen as two separate entities
d = 0.61 wvlength / N.A
d = resolution N.A = n x sin(a) numerical aperture
Osmosis
high to low concentration with SELECTIVELY PERMEABLE membrane
Solvent
dissolving agent
Solute
dissolved substance
Tonicity
non penetrating solutes !! relative concentration of solutions inside and outside cell, determines direction of water flow
Hypertonic
solution with HIGHER SOLUTE concentration
Hypotonic
LOWER SOLUTE concentration
What was inside beaker ?
water and iodine solutions
What was in dialysis bag ?
glucose and starch
Benedicts test
tests for glucose
Iodine test
test for starch
In the exp the solution was…
HYPOtonic
What could pass through the membrane ?
glucose, iodine, water
Starch could not pass through bc the membrane is…
non penetrating
Blue color…
NO glucose
Orange/ red brown….
glucose present
What can you infer ab the size of glucose and iodine particles?
theyre smaller
The potatoes got lighter in what solution….
FRUCTOSE, water being pulled out
in osmosis flow goes from….
HYPO to HYPER
In the 0.6 solution it was…
HYPERTONIC, potato lost weight
How do we know glucose left bag ?
positive benedicts test on beaker solution
Independent variable for enzyme lab….
pH and temperature
Dependent in enzyme lab….
time for starch to disappear
Product of enzyme lab….
maltose and other sugars
When wells turned amber….
high enzyme activity
Control variable of enzyme lab….
room temp, pH 7
ratio for mitosis (chromatids per chromosome)
G1 - 1 S -2 G2 - 2 pro - 2 meta - 2 ana - 1 telo - 1
Mitotic Index
P + M + A + T / total # of cells
Amylase breaks down…
starch
Photosynthesis
carbon dioxide and water combine using sunlight…. produce glucose and oxygen