Midterm 1C

Question 1

The notable dude for first cell culture + what he found out

Answer 1

Ross Harrison
cultures need: anchorage + specific nutrients + sterility (due to slow growth)

Question 1

serum - How is it obtained

Answer 1

blood sample –> allow clotting action by fibrinogen –> pellet cells/clot –> filter off cells

Question 2

why animal cells to excrete mammalian proteins?

Answer 2

cheaper than raw synthesis or using bacteria
excreted proteins have correct folding + mods to allow proper/desired protein functionality with little/no further manipulation by researcher requried

Question 3

define iPSC - How to get?

Answer 3

induced pluripotent stem cells
adult stem cells induced to pluripotency using genetic reprogramming, TFs and hormone conditioning

Question 4

define cellular agriculture

Answer 4

industrial production and harvesting of cellular products (eg proteins)

Question 5

types of cell agri

Answer 5

cellular - plant/animal cell agri –> usually to harvest tissue culture
acellular - bacterial/yeast agri –> usually to harvest proteins/excretions

Question 6

cell agri requirements

Answer 6

cells
scaffolding support
media (w/antibiotics)
bioreactor

Question 7

steps for cultivated meat by cell agri

Answer 7

biopsy to get explant
isolate muscle stem cells
stem cell proliferation
myofiber differentiation
harvest myofibers
processing to final meat product (shaping + adding other compounds for flavour)