Midterm Flashcards
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Which of the following things should you do upon entering the Biology lab room? Select all that apply.
-Put all of your belongings except lab-specific documents in the cubbies at the front of the room.
-Spit out your gum
What should you do to clean your lab bench once you’re finished a lab in which microbes were used?
Wipe down your lab bench with ‘Neutral Q’ antiseptic spray and paper towels
If microbes are used in the lab, what should you use to clean the lab keyboards / mice?
Clorox/Lysol wet-wipes
You let you instructor know that you accidentally broke a glass test tube filled with live E.coli bacteria. Now what should you do?
Spray the shards of glass with a lot of Neutral Q, let it set for 10 minutes to kill all the bacteria, and then with gloved hands, carefully transfer the broken glass and any contaminated paper towels to biohazard bags
You splash methylene blue dye in your face and eyes. What should you do?
Use an eye wash sprayer to wash out your eyes
You are in the middle of a lab when one of your eyes or contact lenses begins bothering you. What should you do?
Wash your hands, then go to the restroom to address the problem
You have long hair. How should you wear your hair in the lab?
Tied back securely such that your hair will not dangle into your work space – this is particularly important when working with open flames, chemicals, or biohazards
Which shoes are appropriate for lab? Select all that apply.
sneakers
Where can you find information about how to dispose properly of lab materials? Select all that apply.
-Your written lab handout/instructions
-Your lab instructor
-A lab manager, if they are available
What is the very last thing you should do as you leave lab?
Wash your hands
What is the correct way to write the scientific name of humans?
Homo sapiens
Put the following taxonomic groupings in order from most inclusive to most exclusive (general to specific).
- Domain
- Kingdom
- Phylum
- Class
- Order
- Family
- Genus
- Species
What is a mnemonic device to keep taxonmic groupings together.
Dear King Philip Came Over For Good Soup
The term that refers to many different species found within a community
biodiversity
Prokaryotic means what?
A general term that describes single-celled organisms.
Eukaryotic means what?
A general term that describes organisms with cells that contain a nucleus.
Fungi means what?
A kingdom entirely made of eukaryotic and mostly multicellular organisms.
Protista means what?
A kingdom entirely of eukaryotic and unicellular organisms.
Metric System Mnemonic Device:
King Henry Died by Drinking Chocolate Milk
What is a meter/
Base unit for measuring length.
What is a liter?
Base unit for measuring volume.
What is a gram?
Base unit for measuring weight.
What is centi-?
Prefix indicating 10^-2 or 0.01 of the base unit.
What is milli-?
Prefix indicating 10^-3 or 0.001 of base unit.
What is micro-?
Prefix indicating 10^-6 or 0.000001 of base unit.
What is kilo?
Prefix indicating 10^3 or 1000 of base unit.
What is nano?
Prefix indicating 10^-9 of base unit.
A measure of molecular motion?
Temp
1/1000 of a meter
millimeter
Curvature of water in a container due to surface tension.
Meniscus.
An apparatus used to measure length.
Ruler.
Technique used to measure small volumes of liquid.
Micropipetting.
When using a spectrophotometer to measure concentration of a solution, which of the following absorbance readings would indicate the highest concentration?
The higher the absorbance rating the higher concentration.
You conduct a Biuret test and your results are purple in color. Which organic compound does the sample contain?
Protein
RNA is an example of what type of organic compound?
Nucleic Acid
An enzyme is usually an example of what type of organic compound?
Protein
The _______ test is used to test for polysaccharides (starches).
Iodine
The building blocks of proteins are
Amino acids
The atom always associated with organic compounds is ________.
Carbon
An example of a fat that would be a liquid at room temperature is __________. You can select more than one answer.
Unsaturated fat
Olive Oil
Each of the following molecules is a subset of Carbohydrates, select the exception. (monosaccharide, polysaccharide, sugar, enzyme)
enzyme
The building blocks of nucleic acids are
nucleotides
You analyze a meat sample that is 87% lean. What percentage would you expect to be fat? (just write the numeral - no % symbol, no decimal).
13
Why did you clean off your work area with Lysol before AND after you plated the bacteria? Select all correct answers.
-To prevent contamination of the bacterial plates
-To prevent the lab benches from being contaminated with the bacteria
An antimicrobial is
- a substance that kills bacteria.
- a substance that inhibits bacterial growth on surfaces.
- a product that stops bacterial infections
In your initial microbe experiment, the independent variable is _________ and the dependent variable is __________.
antiseptic product type / zone of inhibition
The area around the soaked paper discs in which no bacteria grow is called a
zone of inhibition
In the antimicrobial experiment, why did we incubate the bacterial plates at 37C specifically?
37C is the ideal temperature for optimum bacterial growth
Nutient agar provides the bacteria with… (select all correct)
-a surface to grow on
-food to eat
Select all the characteristics that pertain to E. coli.
- gram-negative bacteria
- rod shaped
-some strains are benign, but other strains can cause disease
When you placed your plates in the incubator, why did you put them in upside down?
To prevent condensation from dripping onto the bacteria culture.
If you are observing a specimen using the High Power objective lens, what is the total magnification of the specimen.
400x
Each of the following is a true of a Dissecting Light Microscope, select the exception.
Allows for light to pass through the specimen.
Below are the three basic shapes of prokaryotic cells?
-Spirillus
-Coccus
-Bacillus
Three of the four organelles listed below are found in plant and animal cells. Select the organelle that is only found in plant cells.
Chloroplast
When returning your microscope to the cabinet, which of the following choices should be completed.
-Lower the stage as far as it can go.
- Replace the dust cover over the microscope.
- Place in the cabinet with number facing OUT.
