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Micro Lab Midterm > Midterm > Flashcards

Midterm Flashcards

1
Q

have a prokaryotic cell type.

A

Archaea

Bacteria

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2
Q

all that have a eukaryotic cell type

A 
Algae
Fungi
Nematoda
Platyhelminthes
Protozoans
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3
Q

all that are animals

A

Nematoda

Platyhelminthes

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4
Q

Which gene is used to molecularly identify Bacteria and Archaea?

A

16s rRNA

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5
Q

Which objective lens should be in position when placing a slide onto the stage or off the stage?

A

4x

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6
Q

Total magnification is calculated by multiplying the objective power in use by

A

the ocular power (10x)

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7
Q

Which part of the microscope can be changed to make a brightfield microscope a darkfield microscope?

A

condenser

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8
Q

Which part of the microscope can be changed to make a brightfield microscope a phase contrast microscope?

A

diaphragm

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9
Q

When converting the field of view from mm to um, the mm measurement is multiplied by which value?

A

1000

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10
Q

Which lenses are attached to a rotatable nosepiece?

A
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11
Q

Which objective lens requires a drop of oil to be used properly?

A
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12
Q

A benefit of this microscope is the ability to clearly view live organisms without stains.

A
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13
Q

This is the capacity of a microscope lens system to accurately distinguish between two separate entities that lie close to each other.

A
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14
Q

Which one of the following phylum is considered an algae?

A
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15
Q

Which of the following is NOT in the group Alveolata?

A
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16
Q

Which one of the following bacterial phyla is Gram positive?

A
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17
Q

Which phylum contains the plant pathogenic fungi known as smuts and rusts?

A
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18
Q

Which phylum is an animal microbe?

A
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19
Q

Which virus classification does not match the description?

A
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20
Q

What is the lowest taxonomic level that 16s rRNA sequence analysis is useful for identification?

A

genus

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21
Q

In DNA extraction, the first step is to

A

Break the cells open to expose the DNA

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22
Q

In DNA extraction, the second step is to

A

Remove lipids and proteins

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23
Q

In DNA extraction, the third step is to

A

Precipitate the DNA with alcohol

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24
Q

Which PCR reagent indicates to the Taq polymerase which part of the DNA should be replicated (amplified)?

A

Primers

25
Q

Which PCR reagent provides the nucleotide/basepairs that are used by the Taq polymerase to make copies of the DNA target?

A

dNTPs

26
Q

Which phase of PCR raises the temperature to break the DNA into single strands?

A

Denaturation Phase

27
Q

Which phase of PCR allows for the primers to form hydrogen bonds with the single stranded DNA

A

Annealing Phase

28
Q

Which phase of PCR uses the Taq polymerase to add complementary nucleotides to the single strand of DNA

A

Extension Phase

29
Q

When identfying a bacteria using BLAST, which metric is used?

A

Percent Identity/Similarity

30
Q

In DNA extraction, the third step is to

A
31
Q

Which of the following is a method for freezing a cell to expose DNA?

A
32
Q

Which type of PCR is used to quantify how much RNA is being produced?

A
33
Q

Which reagent of PCR creates covalent bonds to make the new DNA strand?

A
34
Q

Which phase of PCR raises the temperature to break the DNA into single strands?

A
35
Q

Which phase of PCR has an optimal temperature at 95 degres Celsius?

A
36
Q

Which genetic locus is typically used to identify fungi and other eukaryotes?

A
37
Q

In Sanger sequencing, which of the following DNA fragments would travel down the capillary system slowest?

A
38
Q

Which of the following is the most widely used molecular tool in biology?

A
39
Q

This BLAST result illustrates how well the inputted sequence matches to sequences in the database.

A
40
Q

Agar, a molecule derived from red algae, is an important ingredient in which type of media?

A

Semi-Solid

41
Q

This type of media contains one or more agents that inhibit the growth of certain microbes but not another.

A

Selective

42
Q

These media are designed to grow a broad spectrum of microbes that do not have special growth requirements.

A

General Purpose

43
Q

This type of media can grow several types of microbes but are designed to bring out visible differences among those microbes.

A

Differential

44
Q

This media is a general purpose media used for nonfastidious microbes including many bacteria and fungi.

A

Nutrient Agar or Broth (NA & NB)

45
Q

This selective and differential media is used to isolate enteric bacteria that hydrolyze urea.

A

Eosin Methylene Blue Agar (EMB)

46
Q

This media is a general purpose media used for bacteria.

A

Trypticase Soy Agar (TSA)

47
Q

This media is a general purpose media used for fungi.

A

Potato Dextrose Agar (PDA)

48
Q

This selective and differential media is used to isolate enteric bacteria that ferment lactose

A

MacConkey’s Agar

49
Q

This selective and differential media is used to isolate Staphylococcus.

A

Mannitol Salt Agar (MSA)

50
Q

These media contain agar for thickening but do not form substrate

A

Semi Solid

51
Q

These media are designed to grow a spectrum of microbes that do not have special growth requirements

A

General Purpose

52
Q

This type of media is used to test the effectiveness of antimicrobial drugs, disinfectants and antiseptics

A

Assay

53
Q

This type of media contain pure chemical nutrients that vary little from one source to another and have a molecular content with an exact formula

A

Synthetic Media

54
Q

When agar media is placed in a Petri dish, it is called a

A

Pour

55
Q

With Gram Staining, Gram positive bacteria will stain as which color

A
56
Q

The selective and differential media is used to isolate Staphylococcus

A

Mannitol Salt Agar (MSA)

57
Q

Which of the following is commonly used to culture algae?

A

Salt Agar

58
Q

Which piece of equipment is used to sterilize media typically

A

Micro Lab Midterm (1 decks)

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