Midterm

Question 1

have a prokaryotic cell type.

Answer 1

Archaea

Bacteria

Question 2

all that have a eukaryotic cell type

Answer 2

Algae
Fungi
Nematoda
Platyhelminthes
Protozoans

Question 3

all that are animals

Answer 3

Nematoda

Platyhelminthes

Question 4

Which gene is used to molecularly identify Bacteria and Archaea?

Answer 4

16s rRNA

Question 5

Which objective lens should be in position when placing a slide onto the stage or off the stage?

Answer 5

4x

Question 6

Total magnification is calculated by multiplying the objective power in use by

Answer 6

the ocular power (10x)

Question 7

Which part of the microscope can be changed to make a brightfield microscope a darkfield microscope?

Answer 7

condenser

Question 8

Which part of the microscope can be changed to make a brightfield microscope a phase contrast microscope?

Answer 8

diaphragm

Question 9

When converting the field of view from mm to um, the mm measurement is multiplied by which value?

Answer 9

1000

Question 10

Which lenses are attached to a rotatable nosepiece?

Question 11

Which objective lens requires a drop of oil to be used properly?

Question 12

A benefit of this microscope is the ability to clearly view live organisms without stains.

Question 13

This is the capacity of a microscope lens system to accurately distinguish between two separate entities that lie close to each other.

Question 14

Which one of the following phylum is considered an algae?

Question 15

Which of the following is NOT in the group Alveolata?

Question 16

Which one of the following bacterial phyla is Gram positive?

Question 17

Which phylum contains the plant pathogenic fungi known as smuts and rusts?

Question 18

Which phylum is an animal microbe?

Question 19

Which virus classification does not match the description?

Question 20

What is the lowest taxonomic level that 16s rRNA sequence analysis is useful for identification?

Answer 20

genus

Question 21

In DNA extraction, the first step is to

Answer 21

Break the cells open to expose the DNA

Question 22

In DNA extraction, the second step is to

Answer 22

Remove lipids and proteins

Question 23

In DNA extraction, the third step is to

Answer 23

Precipitate the DNA with alcohol

Question 24

Which PCR reagent indicates to the Taq polymerase which part of the DNA should be replicated (amplified)?

Answer 24

Primers

Question 25

Which PCR reagent provides the nucleotide/basepairs that are used by the Taq polymerase to make copies of the DNA target?

Answer 25

dNTPs

Question 26

Which phase of PCR raises the temperature to break the DNA into single strands?

Answer 26

Denaturation Phase

Question 27

Which phase of PCR allows for the primers to form hydrogen bonds with the single stranded DNA

Answer 27

Annealing Phase

Question 28

Which phase of PCR uses the Taq polymerase to add complementary nucleotides to the single strand of DNA

Answer 28

Extension Phase

Question 29

When identfying a bacteria using BLAST, which metric is used?

Answer 29

Percent Identity/Similarity

Question 30

In DNA extraction, the third step is to

Question 31

Which of the following is a method for freezing a cell to expose DNA?

Question 32

Which type of PCR is used to quantify how much RNA is being produced?

Question 33

Which reagent of PCR creates covalent bonds to make the new DNA strand?

Question 34

Which phase of PCR raises the temperature to break the DNA into single strands?

Question 35

Which phase of PCR has an optimal temperature at 95 degres Celsius?

Question 36

Which genetic locus is typically used to identify fungi and other eukaryotes?

Question 37

In Sanger sequencing, which of the following DNA fragments would travel down the capillary system slowest?

Question 38

Which of the following is the most widely used molecular tool in biology?

Question 39

This BLAST result illustrates how well the inputted sequence matches to sequences in the database.

Question 40

Agar, a molecule derived from red algae, is an important ingredient in which type of media?

Answer 40

Semi-Solid

Question 41

This type of media contains one or more agents that inhibit the growth of certain microbes but not another.

Answer 41

Selective

Question 42

These media are designed to grow a broad spectrum of microbes that do not have special growth requirements.

Answer 42

General Purpose

Question 43

This type of media can grow several types of microbes but are designed to bring out visible differences among those microbes.

Answer 43

Differential

Question 44

This media is a general purpose media used for nonfastidious microbes including many bacteria and fungi.

Answer 44

Nutrient Agar or Broth (NA & NB)

Question 45

This selective and differential media is used to isolate enteric bacteria that hydrolyze urea.

Answer 45

Eosin Methylene Blue Agar (EMB)

Question 46

This media is a general purpose media used for bacteria.

Answer 46

Trypticase Soy Agar (TSA)

Question 47

This media is a general purpose media used for fungi.

Answer 47

Potato Dextrose Agar (PDA)

Question 48

This selective and differential media is used to isolate enteric bacteria that ferment lactose

Answer 48

MacConkey's Agar

Question 49

This selective and differential media is used to isolate Staphylococcus.

Answer 49

Mannitol Salt Agar (MSA)

Question 50

These media contain agar for thickening but do not form substrate

Answer 50

Semi Solid

Question 51

These media are designed to grow a spectrum of microbes that do not have special growth requirements

Answer 51

General Purpose

Question 52

This type of media is used to test the effectiveness of antimicrobial drugs, disinfectants and antiseptics

Answer 52

Assay

Question 53

This type of media contain pure chemical nutrients that vary little from one source to another and have a molecular content with an exact formula

Answer 53

Synthetic Media

Question 54

When agar media is placed in a Petri dish, it is called a

Answer 54

Pour

Question 55

With Gram Staining, Gram positive bacteria will stain as which color

Question 56

The selective and differential media is used to isolate Staphylococcus

Answer 56

Mannitol Salt Agar (MSA)

Question 57

Which of the following is commonly used to culture algae?

Answer 57

Salt Agar

Question 58

Which piece of equipment is used to sterilize media typically