MiDie Flashcards
Blood Culture Growth
thick pellicle forms on the surface of medium with hemolysis
Bacillus species
Saprophytic fungi (Candida caligans)
Blood Culture Growth
Less gas but with foul odor
Bacteroides species (B. fragilis)
Blood Culture Growth
Marked hemolysis with unpleasant odor and gas under pressure
Clostridium species (C.pefringens)
Blood Culture Growth
Turbid with marked hemolysis of blood
Beta hemolytic — Streptococci species (S. pyogenes, S.agalactiae)
E. faecalis
Blood Culture Growth
Less distinct turbidity with greenish tint
Alpha hemolytic
S.pneumoniae
N.meningitidis
Blood Culture Growth
No change in the medium only by sub-culturing on chocolate
Haemophilus species (H.influenzae)
–smallest coccobacilli
Blood Culture Growth
Uniformly turbid medium with gas bubbles
Gram negative rods
Blood Culture Growth
Large jelly-like coagulum throughout broth
S.aureus
Blood Culture Growth
Cotton ball colonies on top of sedimented red cell with clear upper layer of broth
Non hemolytic
Streptococci species (S.bovis)
Duration of Culture of Bacteremia & Septicemia
5-10 days
Culture medium for Bacteremia & Septicemia
Aerobic or anaerobic
Culture medium of Brucellosis in blood culture
Brucella Broth or Wisconsin Medium
Duration of culture for Brucellosis in blood
3 to 4 weeks
Culture medium for Leptospirosis in Blood culture
Fletchers medium
EMJH- Ellinghansen McCullough Johnson Harris
Leptospirosis duration of culture
5 to 6 weeks
Neutralizers for sulfonamides antibiotic
Para amino benzoic acid/PABA
Neutralizers for tetracycline antibiotic
MAGNESIUM SULFATE
Ratio of blood to culture medium
1:10
Characteristic of blood anticoagulant
Prevent phagocytosis
Prevent complement activation
Neutralizes some antibiotics
Panton-Valentein Factor
(for destroying WBC)
Leukocidin
Scalded-Skin Syndrome or Ritters disease toxin
Exfoliatin
responsible for food poisoning toxin
Enterotoxin A,B,D
females in use of tampon cause toxin
TSST-1
Spreading Factor
Hyaluronidase
Lipase
DNAse
Protease
Responsible for destroying CLOT
Fibronolysin or Staphylokinase
Disease associated in E.faecalis and E.faecium
I. Endocarditis
II. UTI
III. Pneumonia
IV. Meningitis
2 only (UTI)
Disease associated with S. pneumoniae
I. Endocarditis
II. UTI
III. Pneumonia
IV. Meningitis
3 and 4 only
Disease associated with S.pyogenes
I. Endocarditis
II. UTI
III. Pneumonia
IV. Meningitis
NOTA
Disease associated with S.bovis
I. Endocarditis
II. UTI
III. Pneumonia
IV. Meningitis
1 and 2 only
S. agalactiae
I. Endocarditis
II. Puerperal fever
III. Neonatal Sepsis
IV. Meningitis
V. UTI
2,3,4
Staphylococcus
I. Pinpoint
II. Beta hemolytic
III. Catalase negative
IV. Bacitracin resistant
2 and 4 only
Micrococcus
I. Pinhead
II. Alpha hemolytic
III. Non glucose fermenter
IV. Bacitracin susceptible
3 and 4
Hippurate test
I. Group A
II. purple color
III. turbid
IV. Group B
2 and 4 only
CAMP test
I. Group A
II. bowtie appearance
III. arrowhead hemolysis
IV. Group B
2,3,4 only
Bacitracin test
I. Group A
II. bowtie appearance
III. arrowhead hemolysis
IV. Group B
1 only
PYR hydrolysis test
I. Group A and D
II. Group A only
III. Group B and D
IV. Group E
1 only
Bile esculin test
I. Group A
II. Group D
III. blackening of medium
IV. Entero and Non entero
V. turbidity
2,3,4 only
Serological test
I. Group A
II. Group D
III. Streptex
IV. agglutination
V. turbidity
1,3,4
Salt tolerance test
I. 6.5% NaCl
II. Group D
III. Non-enterococci
IV. Enterococci
V. turbidity
1,2,4,5
Diagnostic test for scarlet fever caused by S.pyogenes
Skin test
✔️Dicks test
✔️Schultz-Charlton Test
Used to test for previous infection of scarlet fever
Dicks Test
Referred as Taxo P
Optochin disc test
Referred as Taxo A
Bacitracin disc test
organisms that resembles Streptococci in terms of MORPHOLOGY
Leuconostoc
Abiotrophia
Granulicatella
used in bioprocessing and biopreservation of foods such as cheese, meats, and vegetables
PEDIOCOCCUS
Nutritionally Variant Streptococci (NVS)
Abiotrophia spp.
Granulicatella spp.
Bile solubility test:
Plated method positive result
disappearance of colonies
Bile solubility test:
Tube method positive result
Turbid
Inulin fermentation positive result
Yellow for S.pneumoniae
Academic/Bergy’s: 10°C and 37°C
Lactic (S. lactic)
Academic/Bergy’s: 37°C
Pyogenic
Viridans
Entero
Lactic
Academic/Bergy’s: 37°C and 45°C
Viridance
Hemolysin that is oxygen stable
Streptolysin S
Academic/Bergy’s: 10°C and 37°C and 45°C
Enterococcus faecalis
Gram negative cocci considered as normal flora of the body
M.catarrhalis
V.parvula
Gram negative cocci that is obligate anaerobe
Veillonella (V.parvula)
Neisseriaceae sp. that is catalase NEGATIVE
N.weaver
Chromogenic neisseriaceae
N. flavescens & N. sicca
Waterhouse-Friderichsen syndrome
Meningococcemia/Spotted Fever
Disseminated Gonorrhoeae
I. Opthalmia neonatorum
II. Gonococcal Arthritis
III. Septicemia
IV. Localized Gonorrhoeae
V. Fitz Hugh-Curtis Syndrome
2, 3, & 5
Veillonella parvula
I. Periodontitis
II. Gonococcal Arthritis
III. Dental Caries
IV. Purulent arthritis
1 & 3
Moraxella catarrhalis
I. Otitis media
II. Broncho-pulmonary infection
III. Bacterial tracheitis
IV. Endocarditis
V. Meningitis
AOTA
N. meningitidis
I. Periodontitis
II. Gonococcal Arthritis
III. Endophthalmitis
IV. Purulent arthritis
3 & 4
commonly used antibiotics for gonorrhea
Penicillin
Ceftriaxone
Spectinomycin
Principal virulence factor of N. meningitidis
Endotoxin (Lipooligosaccharide)
Principal virulence factor of N. gonorrhoeae
Pili
Incubation period of N. gonorrhoeae
2 to 7 days
Human oral and gastrointestinal microbiota
Bacteremia
Endocarditis
Otitis media
Satellitism phenomenon
-Thiol compounds
-cysteine
-vitamin B
-Sulfhydryl compounds or pyridoxal hydrochloride
VF
Mediate acquisition of iron for bacterial metabolism
Transferrin
Lactoferrin
Hemoglobin
protein I
Porin protein- promotes
intracellular survival by preventing phagolysosome fusion in neutrophils
protein II
Opacity protein- mediates firm attachment to eukaryotic cells
Protein III
Reduction-modifiable protein – protects other surface antigens (Por protein, LOS) from bactericidal antibodies
Hydrolyzes beta lactam ring in penicillin
Beta lactamase
Least Virulent serological type of N.meningitidis that causes pneumonia
A. Serotype A
B. Serotype B
C. Serotype C
D. Serotype E
NOTA — Serotype Y
Most Virulent serological type of N.meningitidis that causes invasive disease Meningococcemia
A. Serotype A
B. Serotype B
C. Serotype C
D. Serotype E
NOTA- Serotype W135
serological type of N.meningitidis that causes meningitis and septicemia
A. Serotype A
B. Serotype B
C. Serotype C
D. Serotype E
Serotype C
Incubation period of Spotter fever syndrome
1 to 10 days
vaccine available for the prevention of meningitis
Menactra vaccine
Menactra vaccine contains
Polysaccharide
Antigen A, C, Y and W-135