MiDie Flashcards
Blood Culture Growth
thick pellicle forms on the surface of medium with hemolysis
Bacillus species
Saprophytic fungi (Candida caligans)
Blood Culture Growth
Less gas but with foul odor
Bacteroides species (B. fragilis)
Blood Culture Growth
Marked hemolysis with unpleasant odor and gas under pressure
Clostridium species (C.pefringens)
Blood Culture Growth
Turbid with marked hemolysis of blood
Beta hemolytic — Streptococci species (S. pyogenes, S.agalactiae)
E. faecalis
Blood Culture Growth
Less distinct turbidity with greenish tint
Alpha hemolytic
S.pneumoniae
N.meningitidis
Blood Culture Growth
No change in the medium only by sub-culturing on chocolate
Haemophilus species (H.influenzae)
–smallest coccobacilli
Blood Culture Growth
Uniformly turbid medium with gas bubbles
Gram negative rods
Blood Culture Growth
Large jelly-like coagulum throughout broth
S.aureus
Blood Culture Growth
Cotton ball colonies on top of sedimented red cell with clear upper layer of broth
Non hemolytic
Streptococci species (S.bovis)
Duration of Culture of Bacteremia & Septicemia
5-10 days
Culture medium for Bacteremia & Septicemia
Aerobic or anaerobic
Culture medium of Brucellosis in blood culture
Brucella Broth or Wisconsin Medium
Duration of culture for Brucellosis in blood
3 to 4 weeks
Culture medium for Leptospirosis in Blood culture
Fletchers medium
EMJH- Ellinghansen McCullough Johnson Harris
Leptospirosis duration of culture
5 to 6 weeks
Neutralizers for sulfonamides antibiotic
Para amino benzoic acid/PABA
Neutralizers for tetracycline antibiotic
MAGNESIUM SULFATE
Ratio of blood to culture medium
1:10
Characteristic of blood anticoagulant
Prevent phagocytosis
Prevent complement activation
Neutralizes some antibiotics
Panton-Valentein Factor
(for destroying WBC)
Leukocidin
Scalded-Skin Syndrome or Ritters disease toxin
Exfoliatin
responsible for food poisoning toxin
Enterotoxin A,B,D
females in use of tampon cause toxin
TSST-1
Spreading Factor
Hyaluronidase
Lipase
DNAse
Protease
Responsible for destroying CLOT
Fibronolysin or Staphylokinase
Disease associated in E.faecalis and E.faecium
I. Endocarditis
II. UTI
III. Pneumonia
IV. Meningitis
2 only (UTI)
Disease associated with S. pneumoniae
I. Endocarditis
II. UTI
III. Pneumonia
IV. Meningitis
3 and 4 only
Disease associated with S.pyogenes
I. Endocarditis
II. UTI
III. Pneumonia
IV. Meningitis
NOTA
Disease associated with S.bovis
I. Endocarditis
II. UTI
III. Pneumonia
IV. Meningitis
1 and 2 only
S. agalactiae
I. Endocarditis
II. Puerperal fever
III. Neonatal Sepsis
IV. Meningitis
V. UTI
2,3,4
Staphylococcus
I. Pinpoint
II. Beta hemolytic
III. Catalase negative
IV. Bacitracin resistant
2 and 4 only
Micrococcus
I. Pinhead
II. Alpha hemolytic
III. Non glucose fermenter
IV. Bacitracin susceptible
3 and 4