Microscopy terms Flashcards
Microscopy
is the technical field of using microscopes to view objects and areas of objects that cannot be seen with the naked eye
Magnification
the ability to view an object as larger - simply a function of making an object appear bigger
Resolution
The amount of detail you can see - ability to tell apart different objects -
Field of View
area viewed when looking into a microscope
Compound light Microscope
- High magnification ranging from 40- 1000x
- Good for viewing thin sections of specimens that are micrometers in length and width: cells, protozoan, some bacteria
- Multiple lenses are able to reveal a great amount of detail in samples
- The light source is underneath the specimen while using a compound microscope
Stereo/Dissecting Microscope
- Very versatile: designed for viewing whole objects (3-dimensional) such as rocks, insects and flowers, but can also be used for viewing prepared slides
- Helpful tool for nature studies
- Lower power than compound microscopes
- Magnification levels range from 10 to 40x
Pocket/Field Microscope
used of field studies - light and portable
SEM Scanning Electron Microscope
- Can magnify over 2,000,000 x
- Electrons bounce off the specimen
- Produces a three dimensional image with great surface detail
TEM Transmission Electron Microscopy
The method of transmission electron microscopy (TEM) is used to examine the characteristics of incredibly small specimens. The method makes use of an accelerated electron beam that travels through a very thin specimen to allow researchers to see characteristics like structure and shape.
Wet Mount
Made for temporary use - made in a lab
prepared slide
made for longtime use - purchased