Microscopy P1

Question 1

What materials do you need to investigate microscopy?

Answer 1

For the basic method
• a microscope
• prepared slides of plant and animal cells.

Question 2

What is the equation of magnification?

Answer 2

image size / real size I / A M

Question 3

What were the two types of microscopes?

Answer 3

• Light (used in schools and investigations)

- Electrons (Higher resolution and more details)

Question 4

Explain how to prepare a slide for microscopy

Answer 4

• Using tweezers
• Put specimen stained with iodine (makes coloured and easier to see)
• Mix water drop with iodine
• Cover slip

Question 5

What components make up a light microscope?

Answer 5

• eyepiece
• coarse adjustment knob
• fine adjustment knob
• light
• stage
• high/ low power objective lenses

Question 6

How can you use a light microscope?

Answer 6

• Use first the lowest powered objective lense
• use the coarse adjustment knob to move just below objective lens
• Look through eyepiece and turn until in focus
• Adjust focus with fine knob until clear image is visible
• Until a greater magnification and higher powered objective lens

Question 7

How should you Draw your Observations?

Answer 7

• Use pencil
• Use at least half the space provided
• Do not colour or shade
• Make sure subcellular structures are proportional
• Use a title and magnification
• Label all important features with straight lines