Microscopy / mitosis Flashcards
Study the images of microscope Identify interphase
one nucleus
What is the cell cycle
- interphase
- prophase
- metaphase
- anaphase
- telophase
- Cytokinesis
Functions of microscope: base
flat surface where light resides
arm
supports microscope
light source
bulb set at base
light control
adjust light
diaphragm lever
adjust amount of light passing through specimen
stage clip
holds slide
coarse adjustment knob
control approximate focus
fine adjustment knob
control sharp focus
ocular lenses
for eyes
nosepiece
rotates objective lens
objective lens
magnifies specimen
what is the magnification that objective lens contains?
- 40x
- 10x
3.40x
4.100x
what is the name of objective lens with 4x?
scanning (smallest lens power)
what is the name of objective lens with 10x?
low power objective
what is the name of objective lens with 40x?
high power objective
what is the name of objective lens with 100x?
oil immersion objective
What is the total magnification (tm) of scanning power?
4X10= 40 tm
What is the total magnification (tm) of low power objective?
100tm
What is the total magnification (tm) of high power objective?
400tm
What is the total magnification (tm) of oil immersion objective?
1000x
what is resolution?
shortest distance of depth that can be focused on specimen
depth of field
The range of depth that can be focused on the specimen
field of view
what you can see looking into the ocular lens
what happens during interphase?
-DNA replication to chromatin
-nuclear envelope is present
what happens during prophase?
- chromatin turns to chromosome
- spindles form
-nuclear envelope is removed
what happens during metaphase?
chromosomes line up
what happens during anaphase?
spindles pull chromosomes to edge
what is cytosol
fluid in the cell