Microscopy and optics Flashcards
Oscillations of light are _______ to the direction of energy transfer.,
Perpendicular
Draw a Light wave with all components
Lecture 1 slide 4
Define a ‘Wave’
The propagation of energy from one place to another
Each particle in a wave preforms a _______ motion
Circular
Define blue and red shift.
In the same way as the doppler effect if an object is moving towards you it will have the wave compressed(in the eyes of an observer) causing the frequency to increase and lowering the wavelength causing blue shift.
Red shift is the inverse
Define the ‘Photoelectric Effect’
When a light wave with significant energy strikes a surface causing electrons to be ejected from that material.
Describe ‘Two photon excitation’ and explain the benefits of it
2 photons within the IR range whose total energy has to be sufficient to excite the fluorescent molecule are used to trigger fluorescence however they have to meet within 1 atto second.
Define ‘specular’ media
Metal mirrors give a single reflection and are called specular
Define ‘defuse media
Cells and tissue are diffuse media
Look at reflected illumination diagram
Good
Look at transmitted illumination diagram
ok
Look at simplified imaging light path
done
What is ‘Khöler illumination’
Koehler Illumination is a process that provides optimum contrast and resolution by focusing and centring the light path and spreading it evenly over the field of view.
Describe the use of a condenser aperture.
Controls how much light gets to the same by blocking high angle light at the position where light is focused to a spot.
Describe an “Anti-reflection coating’ (ARC)
ARC reflect light so that incident and reflect waves are out of phase causing destructive interference.
