Microscopy and Cellular Diversity

Question 1

Light Microscope

Answer 1

Uses visible light that is magnified and passed through glass lenses to view the specimen

Question 2

Electron Microscope

Answer 2

Uses an electron beam passed through magnetic lenses to project onto a film or computer for viewing

Question 3

compound microscope

Answer 3

A type of LIGHT MICROSCOPE that is used to observe SMALL objects

Question 4

Ocular Lens

Answer 4

The lens you look through (10 - 15 x magnification)

Question 5

low power objective lens

Answer 5

10 x magnification

Question 6

medium power objective lens

Answer 6

40 x magnification

Question 7

high power objective lens

Answer 7

100 x magnification

Question 8

resolution

Answer 8

ability to resolve detail + distinguish objects that are close together

Question 9

Contrast

Answer 9

the differential absorption of light by diff parts of the object being viewed

Question 10

Stereomicroscope (Dissecting Scope)

Answer 10

Used for observing larger specimen. Has a single set of adjustable OCULAR lenses that gives a 3D view of specimen. Used the light reflected off the specimen.

Question 11

Millimeter (mm)

Answer 11

.001 m (10^-3 m)

Question 12

Micrometer (um)

Answer 12

.000001 m (10^-6 m)

Question 13

Coarse Focus

Answer 13

Large dial on side of microscope used to focus in on specimen (only to be used with low power objective lens)

Question 14

Fine Focus

Answer 14

Small knob used to make small adjustments to focus on given specimen

Question 15

Removing nosepiece

Answer 15

Holds objective lens, used to rotate them

Question 16

Slide Clip

Answer 16

Used to hold slide in place

Question 17

Stage

Answer 17

Where the specimen lays on

Question 18

Light Source

Answer 18

Located near the base of the microscope, used to send light onto the specimen

Question 19

Diaphragm

Answer 19

Located directly underneath the stage, varies intensity of light projected on stage

Question 20

scanning lens

Answer 20

one of the objective lens (4 x magnification)

Question 21

working distance

Answer 21

Distance between slide and bottom of lens

Question 22

Total magnification

Answer 22

Objective lens magnification x ocular lens magnification

Question 23

Diameter of field using scanning lens x total magnification using the scanning lens

Answer 23

Diameter of field using low power lens x total magnification using low power lens