Microscopy and Cellular Diversity Flashcards
Light Microscope
Uses visible light that is magnified and passed through glass lenses to view the specimen
Electron Microscope
Uses an electron beam passed through magnetic lenses to project onto a film or computer for viewing
compound microscope
A type of LIGHT MICROSCOPE that is used to observe SMALL objects
Ocular Lens
The lens you look through (10 - 15 x magnification)
low power objective lens
10 x magnification
medium power objective lens
40 x magnification
high power objective lens
100 x magnification
resolution
ability to resolve detail + distinguish objects that are close together
Contrast
the differential absorption of light by diff parts of the object being viewed
Stereomicroscope (Dissecting Scope)
Used for observing larger specimen. Has a single set of adjustable OCULAR lenses that gives a 3D view of specimen. Used the light reflected off the specimen.
Millimeter (mm)
.001 m (10^-3 m)
Micrometer (um)
.000001 m (10^-6 m)
Coarse Focus
Large dial on side of microscope used to focus in on specimen (only to be used with low power objective lens)
Fine Focus
Small knob used to make small adjustments to focus on given specimen
Removing nosepiece
Holds objective lens, used to rotate them
Slide Clip
Used to hold slide in place
Stage
Where the specimen lays on
Light Source
Located near the base of the microscope, used to send light onto the specimen
Diaphragm
Located directly underneath the stage, varies intensity of light projected on stage
scanning lens
one of the objective lens (4 x magnification)
working distance
Distance between slide and bottom of lens
Total magnification
Objective lens magnification x ocular lens magnification
Diameter of field using scanning lens x total magnification using the scanning lens
Diameter of field using low power lens x total magnification using low power lens
