Microscopy and Cells Flashcards

1
Q

Why does histology have value in diagnosis?

A

It has great value because it is definitive. It means we can be certain what something is and therefore about what is the best treatment.

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2
Q

Define Biopsy.

A

The removal of a small piece of tissue from the body for examination under a microscope.

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3
Q

What are the 6 types of Biopsy?

A

Smear - Cervix/ Buccal Cavity
Curettage (spoon with sharp edge) - Endometrium of Uterus
Needle - large bored and comes out with tissue inside (organs)
Direct Incision - skin/mouth/larynx
Endoscopic - pincers remove tissue (lung/intestine/bladder)
Trans-vascular - pincers through blood vessel (heart/liver)

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4
Q

Define a tissue.

A

A collection of cells which are specialised to perform a particular function.

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5
Q

What is a microtome?

A

It is an instrument used to cut tissue sections so that they can be examined under the microscope.

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6
Q

What is the most common stain and what does it stain?

A

Haemotoxylin and Eosin.

H stains acidic components (such as the nucleus) purple and E stains basic components (cytoplasmic proteins) pink.

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7
Q

Periodic Acid Schiff stains mucus and mucus secreting glands a prominent colour. What is this?

A

Magenta

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8
Q

Why do biopsies need to be fixed?

A

A fresh biopsy is very wet and enzymes are present which will break down the structures. Glutaraldhyde/ Formaldehyde can be used to link macromolecules and so this preserves cellular structure and also kills any bacteria present.

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9
Q

Outline the basics of tissue processing.

A

Tissues need to be embedded in wax to be sectioned.
To get to this point they undergo
Dehydration - cleaning with ethanol - xylene/ toluene (wax adhesive)
Then: the reverse happens so it can be stained because most stains are water soluble.
Then: process happens again so that section does not degrade.

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10
Q

Why can tissue processing lead to shrinkage artefacts?

A

When tissue undergoes processing it goes through a number of chemical process and this can cause different parts of the sample to behave in different ways which can lead to changes of size - shrinkage artefacts

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11
Q

What is phase contrast microscopy?

A

This uses the interference effects when two waves combine to produce clearer images. This can be used to detail living cells.

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12
Q

What is dark field microscopy?

A

This is where an image is created from light scattering patterns.

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13
Q

How does fluorescent microscopy work?

A

It uses 3 stains which are fluorescent and can be used to highlight different parts of the cell depending on which antibodies they are coupled to.

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14
Q

What is the benefit of confocal microscopy?

A

It eliminates out of focus flare. It scans specimens with a laser beam and is able to produce 2D images which can then be built up into a 3D image. This process can be used on living specimens.

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15
Q

What is the Limit of Resolution?

A

Minimum distance at which two objects can be distinguished.

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16
Q

Why is the cell membrane impermeable to water soluble molecules?

A

The cell membrane is a phospholipid bilayer. The middle region is where the hydrophobic tails of the molecules are and so these will not allow water soluble molecules to pass through.

17
Q

What is the glycocalyx?

A

The glycocalyx is the name for the sugars on the outside of the cell membrane. These give specificity to protein action.

18
Q

What is synthesised in the smooth endoplasmic recticulum?

A

Lipids

19
Q

Where does protein synthesis occur in the cell?

A

It occurs in the RER where there are ribosomes to facilitate translation.

20
Q

What are the names of the three Cisternae in the Golgi network?

A

Cis, medial, trans

21
Q

What is the function of the Golgi complex?

A

To modify, sort, concentrate and package proteins/lipids synthesised in the ER.

22
Q

What are the three destinations for molecules passing through the Golgi?

A

Signal mediated diversion to lysosomes
Constitutive secretion into Intracellular space.
Signal mediated diversion to secretory vesicles for regulated secretion.

23
Q

What do lysosomes contain?

A

Lysosomes contain hydrolytic enzymes and they fuse with material that requires digestion. The glycocalyx of the plasma membrane prevents them breaking this down.

24
Q

Where are peroxisomes found?

A

Peroxisomes are found in the liver and kidneys and are responsible for detoxifying reactions by oxidation.

25
Q

In what type of cells will abundant mitochondria be found?

A

Cells which require large amounts of energy as this is where ATP is synthesised.

26
Q

What are micro filaments in the cell cytoskeleton?

A

They are cortical actin filaments of 5-9nm in diameter which lie under the plasma membrane and travel up micro villi and support their shape.

27
Q

What forms a tough mesh in the cytoplasm and provides structure and support?

A

Intermediate filaments.

28
Q

Where are micro tubules found?

A

These are long hollow tubular cylinders which are found in structures which move such a nerve fibres, mitotic spindle, cilia and flagella.

29
Q

What arrangement is seen in micro tubules in flagella/cilia?

A

9+2 arrangement.
The outer arms are able to make and break contact and this allows the cilia to beat. There are nine in a ring round the outside and two in the middle.