Microscopy Flashcards
1
Q
What are the 5 main components of a microscope?
A
- light source
- detector
- stage to hold the sample
- objective lens
- light-conditioning system
2
Q
What 2 parameters must be controlled in live imaging?
A
Carbon dioxide and Temperature
- live samples will want to be in their optimum condition
- small variations can change the plane of the stand, stage and objective - changing the focus
3
Q
Which microscopy technique is related to Stoke’s shift?
A
Fluorescence
- the difference between the wavelength absorbed vs emitted
4
Q
Magnification vs numerical aperture
A
Magnification = the capability of a specific objective to increase the size of the specimen when observed NA = degree of definition an objective can give us
5
Q
What is confocal microscopy?
A
- removes out of focus light by adding a spatial pinhole in the confocal plance
6
Q
Advantages and disadvantages of confocal microscopy
A
- Higher Z-resolution and reduced out-of-focus blur
- Only a thin portion of the sample can be imaged at once
