Microscopy

Question 2

What is the first step in preparing a microscope slide?

Answer 2

Use a dropping pipette to put a drop of water on the microscope slide

This step is essential for providing a medium for the specimen.

Question 3

What tissue should be peeled off for the microscope slide preparation?

Answer 3

A thin layer of epidermal tissue

Epidermal tissue is commonly used for observing plant cells.

Question 4

What tool is used to place the thin layer of tissue on the slide?

Answer 4

Forceps

Forceps allow for careful handling of delicate tissues.

Question 5

How many drops of iodine solution should be added to the tissue?

Answer 5

2 drops of iodine solution

Iodine is used as a stain to enhance visibility of the cells.

Question 6

What should be done after placing the iodine solution on the tissue?

Answer 6

Carefully lower the coverslip

Lowering the coverslip helps avoid air bubbles.

Question 7

What should be used to soak any excess liquid after preparing the slide?

Answer 7

Paper

This helps in maintaining a clean and clear slide for observation.

Question 8

Where should the prepared slide be placed for observation?

Answer 8

On the stage

The stage is the platform where slides are positioned for viewing.

Question 9

Which objective lens should be used first?

Answer 9

The lowest power objective lens

Starting with the lowest power allows for a broader view of the specimen.

Question 10

What knob is used to move the objective lens initially?

Answer 10

Coarse adjustment knob

This knob allows for quick focusing by moving the stage significantly.

Question 11

What knob is used to bring the cells into fine focus?

Answer 11

Fine adjustment knob

The fine adjustment knob provides precise focusing for clear viewing.

Question 12

What is the final step in the microscope slide preparation process?

Answer 12

Make a clear, labelled drawing of some cells

Documenting observations is crucial for analysis and study.