Microscopy

Question 1

What is Kohler illumination?

Answer 1

Kohler illumination is used in light microscopy to evenly illuminate a specimen.

Question 2

How do you use Kohler illumination?

Answer 2

The condenser knob is used to focus the light on the specimen and use the field
diaphragm to control the illuminated area.

Question 3

When do you use Kohler illumination?

Answer 3

It is used when taking precise measurements or taking pictures of a specimen.

Question 4

What does parfocal mean?

Answer 4

Lens that stay in focus when you change the magnification. Allows you to shift
between magnification without the need to refocus.

Question 5

What are dissection microscopes used for?

Answer 5

Dissecting microscopes are used for larger, 3D specimens (insects, plants. etc.).

Question 6

What kind of magnification and light sources do dissection microscopes have?

Answer 6

Possesses low magnification, usually between 5x-50x magnification. Also
possesses two light sources (one at the top is reflective and one at the bottom is
transmitted.

Question 7

What are compound microscopes used for

Answer 7

Compound microscopes are used to magnify small, microscopic specimens on a
flat plane (cells, bacteria, etc.).

Question 8

What magnification do compound microscopes have?

Answer 8

Provides high magnification, from 40x-1000x.

Question 9

6 basic rules when handling microscopes

Answer 9

Only adjust coarse focus at the lowest magnification
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Carry with two hands, one at the base and one at the arm.
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Use proper cleaning tissue when cleaning lenses.
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Don’t drag microscopes across a surface. Lift to move.
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Secure slides using the clips to ensure stabilization