microscopy

Question 1

what is stokes law?

Answer 1

flourescence emission occurs at a longer wavelength than that of excitation light

Question 2

what is limiting microscopy?

Answer 2

diffraction limit

Question 3

how does super resolution microscopy work?

Answer 3

fluorophores are randomly activated ir switched in/off in consecutives acquisitions ..> collection of thousand ioof camera frames and computational processing ..> detect single molecules

Question 4

what is meant with “pleiomorphy of influenza A virions”?

Answer 4

virions exhibit diverse morphology –> different compositions

Question 5

How can virus entry be tracted? Give virus example

Answer 5

-receptor clustering during entry (influenza)
-tracking viral fusion by dye dequenching due to ph changes (influenza)
-tracking incoming viral genomes (adenovirus)

use of click chemistry and super resolution microscopy

Question 6

what are click ligations?

Answer 6

high yielding reactions that are wide in scope and simple to perform

Question 7

how can viral replication organelles studied?

Answer 7

-FISH
-FRAP
-super resolution microscopy

Question 8

Name viral replication organelles and the viruses

Answer 8

-membrane enclosed replication organelles: SARS.CoV-2
-liquid organelles: Rabies virus
-maintenance of viral genome via tethering to hosz chromatin: papillomavirus

Question 9

what is superinfection exclusion? Name Virus example

Answer 9

A cell infected with a virus becomes resistant to secondary infections with the same (homologous) virus, whereas infection with nonrelated (heterologous) viruses is frequently unaffected –> HCV, vaccinia