Brainscape's Knowledge GenomeTM

Browse over 1 million classes created by top students, professors, publishers, and experts.


See full index

Bacte Lab > Microscopy > Flashcards

Microscopy Flashcards

1
Q

Microscopic diagnosis for bacteria

A

Bright-field M: +
Fluorescence M: +/-
Phase-contrast M: +
Dark-field M: +/- [spirochetes (borrelia, leptospira, treponema)]
Electron M: -

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

Microscopic Diagnosis for Fungi

A

Bright-field M: +
Fluorescence M: +/-
Phase-contrast M: +
Dark-field M: -
Electron M: +/-

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

Microscopic diagnosis for Parasites

A

Bright-field M: +
Fluorescence M: +/-
Phase-contrast M: +
Dark-field M: -
Electron M: +/-

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

Microscopic diagnosis for viruses

A

Bright-field M: -
Fluorescence M: +/-
Phase-contrast M: -
Dark-field M: -
Electron M: +/-

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

Used for stained and unstained samples

A

BRIGHT FIELD MICROSCOPE

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

Most common methods employed under bright field microscope

A

o Gram Stain
o Acid Fast Stain
o Potassium Hydroxide

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

Designed to provide maximum illumination and resolution when observing images using a microscope

A

Kohler Illumination

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

Scanning power

Objective lens magnification:
Ocular lens magnification:
Total Magnification:

A

Objective lens magnification: 4x
Ocular lens magnification: 10x
Total magnification: 40x

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

Low power objective lens

Objective lens magnification:
Ocular lens magnification:
Total magnification:

A

O bjective lens magnification: 10x
Ocular lens magnification: 10x
Total magnification: 100x

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

High-dry power objective lens

Objective lens magnification:
Ocular lens magnification:
Total magnification:

A

Objective lens magnification: 40x
Ocular lens magnification: 10x
Total magnification: 400x

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

Oil immwersion power objective lens

Objective lens magnification:
Ocular lens magnification:
Total magnification:

A

Objective lens magnification: 100x
Ocular lens magnification: 10x
Total magnification: 1000x

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

Observing microbial pathogens

Tools: eyes
Magnification: 0
Application:

A

Gross examination

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

Observing microbial pathogens

Tools: Magnifying glass
Magnification: 5
Application:

A

Gross examination

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

Observing microbial pathogens

Tools: dissecting microscope
Magnification: 2.5-30
Application:

A

Gross detailed examination & manipulation

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

Observing microbial pathogens

Tools: bright-field microscope
Magnification: 10-2000
Application:

A

Cells stained

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

Observing microbial pathogens

Tools: dark-field microscope
Magnification: 10-400
Application:

A

Cells not readily stained for bright-field microscopy

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
17
Q

Observing microbial pathogens

Tools: phase-contrast microscope
Magnification: 10-400
Application:

A

Living or unstained cells

18
Q

Observing microbial pathogens

Tools: fluorescence microscope
Magnification: 10-400
Application:

A

Preparations using fluorochrome stains, which can directly stain cells or be connected to antibodies that attach to cells

19
Q

Observing microbial pathogens

Tools: transmission electron microscope
Magnification: 150-10Million
Application:

A

Determine ultra structure of cell organelles

20
Q

Observing microbial pathogens

Tools: scanning electron microscope
Magnification: 20-10,000
Application:

A

Determine surface shapes and structures

21
Q

• Motility of Spirochetes
• Also used for fluorescent stains – Substitute
• Higher resolving power than bright field

A

DARK FIELD MICROSCOPE

22
Q

• Inclusion bodies seen on virus and Chlamydia, living cells/natural state
• Micro lymphocytotoxicity test for Human leukocyte antigen (inverted PCM)

A

PHASE-CONTRAST MICROSCOPE

23
Q

• Requires fluorescent stain
• UVL – provided by mercury are lamp
• Substitute: Dark-field microscopy

A

FLUORESCENT MICROSCOPE

24
Q

Stain cell wall of fungi (Chitin)

A

Calcofluor White

25
Q

Stain high affinity with nucleic acid

A

Acridine Orange

26
Q

Truant’s method; High affinity with mycolic acid (Mycobacterium spp.)

A

Auramine Rhodamine

27
Q

For immunofluorescent techniques; 3 bacteria (Bordetella, Chlamydia, Legionella)

A

Fluorescein isothiocyanate (FITC)

28
Q

• Viral morphology
• Resolution: 0.5 nm
• Fixatives: Glutaraldehyde and Osmium tetroxide
• Dehydrating agents: Alcohol
• Stain: Heavy metals (Lead citrate, uranyl acetate)

A

ELECTRON MICROSCOPE

29
Q

• Transmission EM – _________ structure
• Scanning EM – ____________ structure

A

• Transmission EM – Internal structure
• Scanning EM – External structure

30
Q

This is useful to obtain high resolution images and for three-dimensional reconstruction of biological models

A

Confocal Microscopy

31
Q

This measures surface features by moving a sharp probe over the object’s surface.

A

Scanning Probe Microscopy

32
Q

two types of scanning probe microscope

A

Scanning tunneling microscope
Atomic force microscope

33
Q

Light microscope source

A

Visible light

34
Q

Electron microscope source

A

Expensive

35
Q

Light microscope vs electron microcope

Medium of transmission

A

Air - High vacuum

36
Q

Light microscope vs electron microcope

Nature of Lens

A

Glass - Electromagnet

37
Q

Light microscope vs electron microcope

Focusing mechanism

A

Lens position is adjusted mechanically

Current to the magnetic lens

38
Q

Light M. vs. Electron M.

Source of Contrast

A

Differential light absorption

Scattering of electrons

39
Q

Light M. vs. Electron M.

Specimen mount

A

Glass slide

Metal grid

40
Q

Best resolution

Light M.

Electron M.

A

0.2um

0.5nm

41
Q

Highest practical magnification

Light M.

Electron M.

A

1000 – 1500

Over 100,000

42
Q

Affordability

Light M.

Electron M.

A

Cheaper

Expensive

Bacte Lab (2 decks)

Brainscape helps you reach your goals faster, through stronger study habits.
© 2024 Bold Learning Solutions. Terms and Conditions