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CLINICAL BACTE > microscopy > Flashcards

microscopy Flashcards

1
Q

Provides morphology and arrangement

A

Simple stain

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2
Q

Provides gram reaction, morphology and arrangement

A

Differential stain

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3
Q

Simple staining method

A

Malachite green
safranin
methylene blue

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4
Q

Prepare smear from clinical specimens

A

Direct smear

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5
Q

Prepare smear from culture

A

Indirect smear

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6
Q

Comma shaped bacilli is called

A

Vibrio

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7
Q

What is not usually encountered and where gram stain is not commonly done

A

Spiral

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8
Q

Micrococcus tetragena

A

Gram positive cocci in tetrads

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9
Q

Example of gram positive cocci in pairs (bean-shaped)

A

Streptococcus pneumoniae

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10
Q

Example of gram negative cocci in pairs (commonly found intracellulary inside PMN)

A

Neisseria spp.
- Neisseria meningitidis
- Neisseria gonorrhoeae

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11
Q

Sarcina lutea

A

Gram positive cocci in sarcinae (cubical packet)

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12
Q

Streptococcus

A

Gram positive cocci in chains

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13
Q

Staphyloccocus

A

Gram positive cocci in clusters

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14
Q

Example of gram positive bacilli in chains

A

Bacillus

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15
Q

Non-fermenters such as Pseudomonas

A

Gram negative bacilli in pairs

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16
Q

Enterics

A

Gram negative bacilli in pairs, singles

17
Q

Example of gram positive bacilli that are large and arranged singly or individually with spores

A
  • Clostridium tetani, Clostridium botulinum
  • Clostridium perfringens
18
Q

Box-car appearance bacilli

A

Clostridium perfringens

19
Q

Whats the smallest pathogenic bacilli in micrometre

A

Haemophilus ducreyi

(02.x05 um)

20
Q

Haemophilus ducreyi

A

Gram negative coccobacilli straight and rod shaped

21
Q

Bacteroides fragilis

A

Gram negative bacilli with rounded ends

22
Q

Gram positive irregular bacilli “club shaped” arranged in Chinese characters x, or y and in palisade, snapping arrangement

A

Corynebacterium

23
Q

Gram positive branching bacilli with bifurcated or spatulated ends

A

Bifidobacterium

24
Q

Gram positive bacilli which is slender, branching and filamentous; partially acid fast

A

Nocardia

25
Q

Gram positive filamentous bacilli with a V-shaped pattern

A

Erysipelothrix rhusiopathiae

26
Q

Streptobacillus moniliformis also known as

A

Necklace bacillus

27
Q

Example of gram negative bacilli arranged in long looped filamentous forms

A

Streptobacillus moniliformis

28
Q

Gram negative fusiform bacilli

A

Fusobacterium nucleatum

29
Q

Gram negative comma shaped bacilli in singles

A

Vibrio

30
Q

Gram negative seagull wing shaped or s-shaped bacilli

A

Campylobacter

31
Q

Gram negative curved bacilli with two or more curves

A

Spirillum

32
Q

Acid fast bacilli
- slender, long, arranged in serpentine cord like pattern

A

Mycobacterium tuberculosis

33
Q

Acid fast bacilli
- slender and in palisades / globi packets / cigar bundle; arranged singly

A

Mycobacterium leprae

34
Q

Gram negative spiral shaped bacteria

  • stained with silver, fluorescent or tissue stains such as giemsa
A

Spirochets

Leptospira interrogans
Treponema pallidum
Borrelia

35
Q

Gram positive (not bacteria)

A

Yeast cells and pseudohypha

36
Q

Square shaped bacteria

A

Haloarcula spp.

37
Q

Star shaped

A

Stella spp.

38
Q

In DSSM, ______ should be read before reporting the sputum smear as negative for AFB

A

300 OIF

39
Q

Spiral shaped structures that can be seen in asthmatics

A

Curschmann’s spirals

CLINICAL BACTE (2 decks)

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