microscopy Flashcards
Provides morphology and arrangement
Simple stain
Provides gram reaction, morphology and arrangement
Differential stain
Simple staining method
Malachite green
safranin
methylene blue
Prepare smear from clinical specimens
Direct smear
Prepare smear from culture
Indirect smear
Comma shaped bacilli is called
Vibrio
What is not usually encountered and where gram stain is not commonly done
Spiral
Micrococcus tetragena
Gram positive cocci in tetrads
Example of gram positive cocci in pairs (bean-shaped)
Streptococcus pneumoniae
Example of gram negative cocci in pairs (commonly found intracellulary inside PMN)
Neisseria spp.
- Neisseria meningitidis
- Neisseria gonorrhoeae
Sarcina lutea
Gram positive cocci in sarcinae (cubical packet)
Streptococcus
Gram positive cocci in chains
Staphyloccocus
Gram positive cocci in clusters
Example of gram positive bacilli in chains
Bacillus
Non-fermenters such as Pseudomonas
Gram negative bacilli in pairs
Enterics
Gram negative bacilli in pairs, singles
Example of gram positive bacilli that are large and arranged singly or individually with spores
- Clostridium tetani, Clostridium botulinum
- Clostridium perfringens
Box-car appearance bacilli
Clostridium perfringens
Whats the smallest pathogenic bacilli in micrometre
Haemophilus ducreyi
(02.x05 um)
Haemophilus ducreyi
Gram negative coccobacilli straight and rod shaped
Bacteroides fragilis
Gram negative bacilli with rounded ends
Gram positive irregular bacilli “club shaped” arranged in Chinese characters x, or y and in palisade, snapping arrangement
Corynebacterium
Gram positive branching bacilli with bifurcated or spatulated ends
Bifidobacterium
Gram positive bacilli which is slender, branching and filamentous; partially acid fast
Nocardia
Gram positive filamentous bacilli with a V-shaped pattern
Erysipelothrix rhusiopathiae
Streptobacillus moniliformis also known as
Necklace bacillus
Example of gram negative bacilli arranged in long looped filamentous forms
Streptobacillus moniliformis
Gram negative fusiform bacilli
Fusobacterium nucleatum
Gram negative comma shaped bacilli in singles
Vibrio
Gram negative seagull wing shaped or s-shaped bacilli
Campylobacter
Gram negative curved bacilli with two or more curves
Spirillum
Acid fast bacilli
- slender, long, arranged in serpentine cord like pattern
Mycobacterium tuberculosis
Acid fast bacilli
- slender and in palisades / globi packets / cigar bundle; arranged singly
Mycobacterium leprae
Gram negative spiral shaped bacteria
- stained with silver, fluorescent or tissue stains such as giemsa
Spirochets
Leptospira interrogans
Treponema pallidum
Borrelia
Gram positive (not bacteria)
Yeast cells and pseudohypha
Square shaped bacteria
Haloarcula spp.
Star shaped
Stella spp.
In DSSM, ______ should be read before reporting the sputum smear as negative for AFB
300 OIF
Spiral shaped structures that can be seen in asthmatics
Curschmann’s spirals
